Methotrexate-loaded biodegradable nanoparticles: Preparation, characterization and evaluation of its cytotoxic potential against U-343 MGa human neuronal glioblastoma cells

Kranti P. Musmade, Praful B. Deshpande, Prashant B. Musmade, M. Naseer Maliyakkal, A. Ranjith Kumar, M. Sreenivasa Reddy, N. Udupa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Nanoparticles represent one of the attractive alternatives in the effective treatment of cancer chemotherapy. In the present work, formulation and development of a novel methotrexate (MTX)-loaded biodegradable nanoparticles using poly(D,L-lactide-co-glycolide) (PLGA) was carried out. The prepared nanoparticles were evaluated for physicochemical properties such as particle size, zeta potential, release studies, etc and also evaluated for its in vitro cytotoxic potential against U-343 MGa human neuronal glioblastoma cells. Particle size of optimized formulation was < 200 nm. There was a considerable decrease in cell viability and enhancement in cytotoxic activity of MTX-loaded nanoparticles compared to MTX alone when tested against U-343 MGa human neuronal glioblastoma cells.

Original languageEnglish
Pages (from-to)945-951
Number of pages7
JournalBulletin of Materials Science
Volume37
Issue number4
Publication statusPublished - 2014

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Methotrexate
Nanoparticles
Particle size
Chemotherapy
Zeta potential
Cells
polylactic acid-polyglycolic acid copolymer

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials

Cite this

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Methotrexate-loaded biodegradable nanoparticles: Preparation, characterization and evaluation of its cytotoxic potential against U-343 MGa human neuronal glioblastoma cells. / Musmade, Kranti P.; Deshpande, Praful B.; Musmade, Prashant B.; Maliyakkal, M. Naseer; Kumar, A. Ranjith; Reddy, M. Sreenivasa; Udupa, N.

In: Bulletin of Materials Science, Vol. 37, No. 4, 2014, p. 945-951.

Research output: Contribution to journalArticle

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AU - Musmade, Kranti P.

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AU - Maliyakkal, M. Naseer

AU - Kumar, A. Ranjith

AU - Reddy, M. Sreenivasa

AU - Udupa, N.

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