Micro-raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells

Aseefhali Bankapur, R. Sagar Krishnamurthy, Elsa Zachariah, Chidangil Santhosh, Basavaraj Chougule, Bhavishna Praveen, Manna Valiathan, Deepak Mathur

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 μg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 μg/ml.

Original languageEnglish
Article numbere35075
JournalPLoS One
Volume7
Issue number4
DOIs
Publication statusPublished - 13-04-2012

Fingerprint

nanosilver
Raman spectroscopy
Raman Spectrum Analysis
nanoparticles
Stem cells
Silver
Nanoparticles
stem cells
Stem Cells
Mesenchymal Stromal Cells
oxidative stress
statistical analysis
cells
Oxidative stress
Raman scattering
Statistical methods
Oxidative Stress
Doping (additives)

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Bankapur, Aseefhali ; Krishnamurthy, R. Sagar ; Zachariah, Elsa ; Santhosh, Chidangil ; Chougule, Basavaraj ; Praveen, Bhavishna ; Valiathan, Manna ; Mathur, Deepak. / Micro-raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells. In: PLoS One. 2012 ; Vol. 7, No. 4.
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Micro-raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells. / Bankapur, Aseefhali; Krishnamurthy, R. Sagar; Zachariah, Elsa; Santhosh, Chidangil; Chougule, Basavaraj; Praveen, Bhavishna; Valiathan, Manna; Mathur, Deepak.

In: PLoS One, Vol. 7, No. 4, e35075, 13.04.2012.

Research output: Contribution to journalArticle

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