Micro-raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells

Aseefhali Bankapur, R. Sagar Krishnamurthy, Elsa Zachariah, Chidangil Santhosh, Basavaraj Chougule, Bhavishna Praveen, Manna Valiathan, Deepak Mathur

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We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 μg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 μg/ml.

Original languageEnglish
Article numbere35075
JournalPLoS One
Issue number4
Publication statusPublished - 13-04-2012


All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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