Microarray analysis of phosphatase gene expression in human melanoma

L. McArdle, M. M. Rafferty, K. Satyamoorthy, G. M. Maelandsmo, P. A. Dervan, M. Herlyn, D. J. Easty

    Research output: Contribution to journalReview article

    17 Citations (Scopus)

    Abstract

    Background: Tyrosine phosphate is abnormally elevated in malignant melanoma, and this has been interpreted to reflect the activity of oncogenic protein tyrosine kinases. However, elevation may also arise due to decreased protein tyrosine phosphatase (PTP) expression. Objectives: To survey phosphatase gene expression in melanoma cell lines, a benign naevus and normal melanocytes: we searched for downregulation of phosphatase gene expression in malignant cells that may indicate a role as melanoma suppressor genes. Methods: Microarray analysis was used to compare gene expression for 133 phosphatase genes, comprising 39 PTPs, 16 dual-specificity phosphatases (DSPs), 47 serine/threonine phosphatases and 31 acid/alkaline and lipid-based phosphatases. Northern blotting analysis was used to study gene expression in human melanoma biopsies. Results: There was decreased expression of four DSP genes (including PTEN); eight receptor PTP genes were downregulated in melanoma, among which were PTP-KAPPA and PTP-PI (consistent with our previous data). In addition, PTP-RF/LAR was downregulated in 13 of 22 metastatic melanomas. Conclusions: The expression of multiple PTP receptors is decreased in melanoma; this may be a mechanism which stimulates autonomous growth in advanced melanoma.

    Original languageEnglish
    Pages (from-to)925-930
    Number of pages6
    JournalBritish Journal of Dermatology
    Volume152
    Issue number5
    DOIs
    Publication statusPublished - 05-2005

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    Microarray Analysis
    Phosphoric Monoester Hydrolases
    Protein Tyrosine Phosphatases
    Melanoma
    Gene Expression
    Dual-Specificity Phosphatases
    Down-Regulation
    Genes
    Suppressor Genes
    Nevus
    Phosphoprotein Phosphatases
    Melanocytes
    Northern Blotting
    Protein-Tyrosine Kinases
    Tyrosine
    Phosphates
    Lipids
    Biopsy
    Cell Line
    Acids

    All Science Journal Classification (ASJC) codes

    • Dermatology

    Cite this

    McArdle, L., Rafferty, M. M., Satyamoorthy, K., Maelandsmo, G. M., Dervan, P. A., Herlyn, M., & Easty, D. J. (2005). Microarray analysis of phosphatase gene expression in human melanoma. British Journal of Dermatology, 152(5), 925-930. https://doi.org/10.1111/j.1365-2133.2005.06454.x
    McArdle, L. ; Rafferty, M. M. ; Satyamoorthy, K. ; Maelandsmo, G. M. ; Dervan, P. A. ; Herlyn, M. ; Easty, D. J. / Microarray analysis of phosphatase gene expression in human melanoma. In: British Journal of Dermatology. 2005 ; Vol. 152, No. 5. pp. 925-930.
    @article{da2ba299c92843289540631c45055231,
    title = "Microarray analysis of phosphatase gene expression in human melanoma",
    abstract = "Background: Tyrosine phosphate is abnormally elevated in malignant melanoma, and this has been interpreted to reflect the activity of oncogenic protein tyrosine kinases. However, elevation may also arise due to decreased protein tyrosine phosphatase (PTP) expression. Objectives: To survey phosphatase gene expression in melanoma cell lines, a benign naevus and normal melanocytes: we searched for downregulation of phosphatase gene expression in malignant cells that may indicate a role as melanoma suppressor genes. Methods: Microarray analysis was used to compare gene expression for 133 phosphatase genes, comprising 39 PTPs, 16 dual-specificity phosphatases (DSPs), 47 serine/threonine phosphatases and 31 acid/alkaline and lipid-based phosphatases. Northern blotting analysis was used to study gene expression in human melanoma biopsies. Results: There was decreased expression of four DSP genes (including PTEN); eight receptor PTP genes were downregulated in melanoma, among which were PTP-KAPPA and PTP-PI (consistent with our previous data). In addition, PTP-RF/LAR was downregulated in 13 of 22 metastatic melanomas. Conclusions: The expression of multiple PTP receptors is decreased in melanoma; this may be a mechanism which stimulates autonomous growth in advanced melanoma.",
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    McArdle, L, Rafferty, MM, Satyamoorthy, K, Maelandsmo, GM, Dervan, PA, Herlyn, M & Easty, DJ 2005, 'Microarray analysis of phosphatase gene expression in human melanoma', British Journal of Dermatology, vol. 152, no. 5, pp. 925-930. https://doi.org/10.1111/j.1365-2133.2005.06454.x

    Microarray analysis of phosphatase gene expression in human melanoma. / McArdle, L.; Rafferty, M. M.; Satyamoorthy, K.; Maelandsmo, G. M.; Dervan, P. A.; Herlyn, M.; Easty, D. J.

    In: British Journal of Dermatology, Vol. 152, No. 5, 05.2005, p. 925-930.

    Research output: Contribution to journalReview article

    TY - JOUR

    T1 - Microarray analysis of phosphatase gene expression in human melanoma

    AU - McArdle, L.

    AU - Rafferty, M. M.

    AU - Satyamoorthy, K.

    AU - Maelandsmo, G. M.

    AU - Dervan, P. A.

    AU - Herlyn, M.

    AU - Easty, D. J.

    PY - 2005/5

    Y1 - 2005/5

    N2 - Background: Tyrosine phosphate is abnormally elevated in malignant melanoma, and this has been interpreted to reflect the activity of oncogenic protein tyrosine kinases. However, elevation may also arise due to decreased protein tyrosine phosphatase (PTP) expression. Objectives: To survey phosphatase gene expression in melanoma cell lines, a benign naevus and normal melanocytes: we searched for downregulation of phosphatase gene expression in malignant cells that may indicate a role as melanoma suppressor genes. Methods: Microarray analysis was used to compare gene expression for 133 phosphatase genes, comprising 39 PTPs, 16 dual-specificity phosphatases (DSPs), 47 serine/threonine phosphatases and 31 acid/alkaline and lipid-based phosphatases. Northern blotting analysis was used to study gene expression in human melanoma biopsies. Results: There was decreased expression of four DSP genes (including PTEN); eight receptor PTP genes were downregulated in melanoma, among which were PTP-KAPPA and PTP-PI (consistent with our previous data). In addition, PTP-RF/LAR was downregulated in 13 of 22 metastatic melanomas. Conclusions: The expression of multiple PTP receptors is decreased in melanoma; this may be a mechanism which stimulates autonomous growth in advanced melanoma.

    AB - Background: Tyrosine phosphate is abnormally elevated in malignant melanoma, and this has been interpreted to reflect the activity of oncogenic protein tyrosine kinases. However, elevation may also arise due to decreased protein tyrosine phosphatase (PTP) expression. Objectives: To survey phosphatase gene expression in melanoma cell lines, a benign naevus and normal melanocytes: we searched for downregulation of phosphatase gene expression in malignant cells that may indicate a role as melanoma suppressor genes. Methods: Microarray analysis was used to compare gene expression for 133 phosphatase genes, comprising 39 PTPs, 16 dual-specificity phosphatases (DSPs), 47 serine/threonine phosphatases and 31 acid/alkaline and lipid-based phosphatases. Northern blotting analysis was used to study gene expression in human melanoma biopsies. Results: There was decreased expression of four DSP genes (including PTEN); eight receptor PTP genes were downregulated in melanoma, among which were PTP-KAPPA and PTP-PI (consistent with our previous data). In addition, PTP-RF/LAR was downregulated in 13 of 22 metastatic melanomas. Conclusions: The expression of multiple PTP receptors is decreased in melanoma; this may be a mechanism which stimulates autonomous growth in advanced melanoma.

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