Microbial assessment of dental unit waterlines in an institutional setup in Karnataka, South India

Shobha Rodrigues, Shivani Suvarna, Jyoti Suvarna, Vishwas Saralaya, Sharon Saldanha, Vidya K. Shenoy

Research output: Contribution to journalArticle

Abstract

Background and Aim: Biofilms in dental unit waterlines (DUWLs), suction hoses, and fittings are a potentially significant source of cross-contamination posing significant health risk as these may come into contact with patients during treatment. The purpose of this in vitro study was to identify the spectrum of bacterial flora colonizing the DUWLs and to detect pathogenic microorganisms present in such an environmental niche. Materials and Methods: Thirty DUWL samples were collected from in use dental units selected randomly from various clinical departments. Samples were collected from the following devices; 3-in-1 syringe waterline, section of waterline tubing supplying the 3-in-1 syringe, and the air rotor water. The samples were subjected to bacteriological analysis, and all bacterial isolates were tested for their ability to form biofilms. Results: A descriptive analysis of the results obtained was carried out, and it was observed that 7 out of 30 (23.3%) samples collected from DUWL were supplying water of unsatisfactory quality with species of low-pathogenicity bacteria isolated present in significant numbers; four of ten (40%) water supply lines contained bacterial biofilms; and the species with greatest capability to form biofilms were Enterobacter species (spp.). In addition, the results were also subjected to Chi-square test which revealed no statistical difference between the species and the location of collection of samples. Conclusion: Within the limitations of this study, it is concluded that DUWLs are not totally free of contamination. Microbial biofilms are a significant source of cross-contamination and cross-infection in the dental clinic environment.

Original languageEnglish
Pages (from-to)555-559
Number of pages5
JournalIndian Journal of Dental Research
Volume28
Issue number5
DOIs
Publication statusPublished - 01-09-2017

Fingerprint

Biofilms
India
Tooth
Syringes
Dental Clinics
Enterobacter
Water Supply
Water Quality
Suction
Chi-Square Distribution
Cross Infection
Virulence
Air
Bacteria
Equipment and Supplies
Water
Health
Therapeutics

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

@article{d2d8104bd31f43e8b3612a6fef81092c,
title = "Microbial assessment of dental unit waterlines in an institutional setup in Karnataka, South India",
abstract = "Background and Aim: Biofilms in dental unit waterlines (DUWLs), suction hoses, and fittings are a potentially significant source of cross-contamination posing significant health risk as these may come into contact with patients during treatment. The purpose of this in vitro study was to identify the spectrum of bacterial flora colonizing the DUWLs and to detect pathogenic microorganisms present in such an environmental niche. Materials and Methods: Thirty DUWL samples were collected from in use dental units selected randomly from various clinical departments. Samples were collected from the following devices; 3-in-1 syringe waterline, section of waterline tubing supplying the 3-in-1 syringe, and the air rotor water. The samples were subjected to bacteriological analysis, and all bacterial isolates were tested for their ability to form biofilms. Results: A descriptive analysis of the results obtained was carried out, and it was observed that 7 out of 30 (23.3{\%}) samples collected from DUWL were supplying water of unsatisfactory quality with species of low-pathogenicity bacteria isolated present in significant numbers; four of ten (40{\%}) water supply lines contained bacterial biofilms; and the species with greatest capability to form biofilms were Enterobacter species (spp.). In addition, the results were also subjected to Chi-square test which revealed no statistical difference between the species and the location of collection of samples. Conclusion: Within the limitations of this study, it is concluded that DUWLs are not totally free of contamination. Microbial biofilms are a significant source of cross-contamination and cross-infection in the dental clinic environment.",
author = "Shobha Rodrigues and Shivani Suvarna and Jyoti Suvarna and Vishwas Saralaya and Sharon Saldanha and Shenoy, {Vidya K.}",
year = "2017",
month = "9",
day = "1",
doi = "10.4103/ijdr.IJDR_775_16",
language = "English",
volume = "28",
pages = "555--559",
journal = "Indian Journal of Dental Research",
issn = "0970-9290",
publisher = "Medknow Publications and Media Pvt. Ltd",
number = "5",

}

Microbial assessment of dental unit waterlines in an institutional setup in Karnataka, South India. / Rodrigues, Shobha; Suvarna, Shivani; Suvarna, Jyoti; Saralaya, Vishwas; Saldanha, Sharon; Shenoy, Vidya K.

In: Indian Journal of Dental Research, Vol. 28, No. 5, 01.09.2017, p. 555-559.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Microbial assessment of dental unit waterlines in an institutional setup in Karnataka, South India

AU - Rodrigues, Shobha

AU - Suvarna, Shivani

AU - Suvarna, Jyoti

AU - Saralaya, Vishwas

AU - Saldanha, Sharon

AU - Shenoy, Vidya K.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Background and Aim: Biofilms in dental unit waterlines (DUWLs), suction hoses, and fittings are a potentially significant source of cross-contamination posing significant health risk as these may come into contact with patients during treatment. The purpose of this in vitro study was to identify the spectrum of bacterial flora colonizing the DUWLs and to detect pathogenic microorganisms present in such an environmental niche. Materials and Methods: Thirty DUWL samples were collected from in use dental units selected randomly from various clinical departments. Samples were collected from the following devices; 3-in-1 syringe waterline, section of waterline tubing supplying the 3-in-1 syringe, and the air rotor water. The samples were subjected to bacteriological analysis, and all bacterial isolates were tested for their ability to form biofilms. Results: A descriptive analysis of the results obtained was carried out, and it was observed that 7 out of 30 (23.3%) samples collected from DUWL were supplying water of unsatisfactory quality with species of low-pathogenicity bacteria isolated present in significant numbers; four of ten (40%) water supply lines contained bacterial biofilms; and the species with greatest capability to form biofilms were Enterobacter species (spp.). In addition, the results were also subjected to Chi-square test which revealed no statistical difference between the species and the location of collection of samples. Conclusion: Within the limitations of this study, it is concluded that DUWLs are not totally free of contamination. Microbial biofilms are a significant source of cross-contamination and cross-infection in the dental clinic environment.

AB - Background and Aim: Biofilms in dental unit waterlines (DUWLs), suction hoses, and fittings are a potentially significant source of cross-contamination posing significant health risk as these may come into contact with patients during treatment. The purpose of this in vitro study was to identify the spectrum of bacterial flora colonizing the DUWLs and to detect pathogenic microorganisms present in such an environmental niche. Materials and Methods: Thirty DUWL samples were collected from in use dental units selected randomly from various clinical departments. Samples were collected from the following devices; 3-in-1 syringe waterline, section of waterline tubing supplying the 3-in-1 syringe, and the air rotor water. The samples were subjected to bacteriological analysis, and all bacterial isolates were tested for their ability to form biofilms. Results: A descriptive analysis of the results obtained was carried out, and it was observed that 7 out of 30 (23.3%) samples collected from DUWL were supplying water of unsatisfactory quality with species of low-pathogenicity bacteria isolated present in significant numbers; four of ten (40%) water supply lines contained bacterial biofilms; and the species with greatest capability to form biofilms were Enterobacter species (spp.). In addition, the results were also subjected to Chi-square test which revealed no statistical difference between the species and the location of collection of samples. Conclusion: Within the limitations of this study, it is concluded that DUWLs are not totally free of contamination. Microbial biofilms are a significant source of cross-contamination and cross-infection in the dental clinic environment.

UR - http://www.scopus.com/inward/record.url?scp=85032889252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032889252&partnerID=8YFLogxK

U2 - 10.4103/ijdr.IJDR_775_16

DO - 10.4103/ijdr.IJDR_775_16

M3 - Article

AN - SCOPUS:85032889252

VL - 28

SP - 555

EP - 559

JO - Indian Journal of Dental Research

JF - Indian Journal of Dental Research

SN - 0970-9290

IS - 5

ER -