Microbial degradation of myo-inositol hexakisphosphate (IP6)

specificity, kinetics, and simulation

Paul Priyodip, Seetharaman Balaji

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Microbial degradation of myo-inositol hexakisphosphate (IP6) is crucial to deal with nutritional problems in monogastric animals as well as to prevent environmental phosphate pollution. The present study deals with the degradation of IP6 by microorganisms such as Sporosarcina spp. pasteurii, globiospora, psychrophila, Streptococcus thermophilus and Saccharomyces boulardii. These microbes were screened for phytase production under laboratory conditions. The specificity of the enzyme was tested for various phosphorylated substrates such as sodium phytate (IP6), sodium hexametaphosphate, phenyl phosphate, α-d-glucose-6 phosphate, inosine 5′ monophosphate and pyridoxal 5′ phosphate. These enzymes were highly specific to IP6. The influence of modulators such as phytochemicals and metal ions on the enzymatic activity was assessed. These modulators in different concentrations had varying effect on microbial phytases. Calcium (in optimal concentration of 0.5 M) played an important role in enzyme activation. The enzymes were then characterized based on their molecular weight 41~43 kDa. The phytase-producing microbes were assessed for IP6 degradation in a simulated intestinal setup. Among the selected microbes, Sporosarcina globiospora hydrolyzed IP6 effectively, as confirmed by colorimetric time-based analysis.

Original languageEnglish
Article number268
Journal3 Biotech
Volume8
Issue number6
DOIs
Publication statusPublished - 01-06-2018

Fingerprint

6-Phytase
Phytic Acid
Sporosarcina
myo-inositol
biodegradation
phytases
phosphate
enzyme
microorganisms
kinetics
degradation
Enzymes
Phosphates
Streptococcus thermophilus
simulation
Inosine Monophosphate
Glucose-6-Phosphate
Environmental Pollution
Pyridoxal Phosphate
Enzyme Activation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Science (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

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abstract = "Microbial degradation of myo-inositol hexakisphosphate (IP6) is crucial to deal with nutritional problems in monogastric animals as well as to prevent environmental phosphate pollution. The present study deals with the degradation of IP6 by microorganisms such as Sporosarcina spp. pasteurii, globiospora, psychrophila, Streptococcus thermophilus and Saccharomyces boulardii. These microbes were screened for phytase production under laboratory conditions. The specificity of the enzyme was tested for various phosphorylated substrates such as sodium phytate (IP6), sodium hexametaphosphate, phenyl phosphate, α-d-glucose-6 phosphate, inosine 5′ monophosphate and pyridoxal 5′ phosphate. These enzymes were highly specific to IP6. The influence of modulators such as phytochemicals and metal ions on the enzymatic activity was assessed. These modulators in different concentrations had varying effect on microbial phytases. Calcium (in optimal concentration of 0.5 M) played an important role in enzyme activation. The enzymes were then characterized based on their molecular weight 41~43 kDa. The phytase-producing microbes were assessed for IP6 degradation in a simulated intestinal setup. Among the selected microbes, Sporosarcina globiospora hydrolyzed IP6 effectively, as confirmed by colorimetric time-based analysis.",
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Microbial degradation of myo-inositol hexakisphosphate (IP6) : specificity, kinetics, and simulation. / Priyodip, Paul; Balaji, Seetharaman.

In: 3 Biotech, Vol. 8, No. 6, 268, 01.06.2018.

Research output: Contribution to journalArticle

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