Microscopic and spectroscopic characterization of rice and corn starch

Indira Govindaraju, Sparsha Pallen, Suchitta Umashankar, Sib Sankar Mal, Sindhoora Kaniyala Melanthota, Dhani Ram Mahato, Guan Yu Zhuo, Krishna Kishore Mahato, Nirmal Mazumder

Research output: Contribution to journalArticle

Abstract

Starch granules from rice and corn were isolated, and their molecular mechanism on interaction with α-amylase was characterized through biochemical test, microscopic imaging, and spectroscopic measurements. The micro-scale structure of starch granules were observed under an optical microscope and their average size was in the range 1–100 μm. The surface topological structures of starch with micro-holes due to the effect of α- amylase were also visualized under scanning electron microscope. The crystallinity was confirmed by X-ray diffraction patterns as well as second-harmonic generation microscopy. The change in chemical bonds before and after hydrolysis of the starch granules by α- amylase was determined by Fourier transform infrared spectroscopy. Combination of microscopy and spectroscopy techniques relates structural and chemical features that explain starch enzymatic hydrolysis which will provide a valid basis for future studies in food science and insights into the energy transformation dynamics.

Original languageEnglish
JournalMicroscopy Research and Technique
DOIs
Publication statusAccepted/In press - 01-01-2020

Fingerprint

corn
starches
rice
Starch
Zea mays
Amylases
hydrolysis
Microscopy
Microscopic examination
Hydrolysis
microscopy
Food Technology
Enzymatic hydrolysis
Chemical bonds
Fourier Transform Infrared Spectroscopy
Harmonic generation
chemical bonds
optical microscopes
food
X-Ray Diffraction

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

Cite this

Govindaraju, I., Pallen, S., Umashankar, S., Mal, S. S., Kaniyala Melanthota, S., Mahato, D. R., ... Mazumder, N. (Accepted/In press). Microscopic and spectroscopic characterization of rice and corn starch. Microscopy Research and Technique. https://doi.org/10.1002/jemt.23437
Govindaraju, Indira ; Pallen, Sparsha ; Umashankar, Suchitta ; Mal, Sib Sankar ; Kaniyala Melanthota, Sindhoora ; Mahato, Dhani Ram ; Zhuo, Guan Yu ; Mahato, Krishna Kishore ; Mazumder, Nirmal. / Microscopic and spectroscopic characterization of rice and corn starch. In: Microscopy Research and Technique. 2020.
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Microscopic and spectroscopic characterization of rice and corn starch. / Govindaraju, Indira; Pallen, Sparsha; Umashankar, Suchitta; Mal, Sib Sankar; Kaniyala Melanthota, Sindhoora; Mahato, Dhani Ram; Zhuo, Guan Yu; Mahato, Krishna Kishore; Mazumder, Nirmal.

In: Microscopy Research and Technique, 01.01.2020.

Research output: Contribution to journalArticle

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AU - Govindaraju, Indira

AU - Pallen, Sparsha

AU - Umashankar, Suchitta

AU - Mal, Sib Sankar

AU - Kaniyala Melanthota, Sindhoora

AU - Mahato, Dhani Ram

AU - Zhuo, Guan Yu

AU - Mahato, Krishna Kishore

AU - Mazumder, Nirmal

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Govindaraju I, Pallen S, Umashankar S, Mal SS, Kaniyala Melanthota S, Mahato DR et al. Microscopic and spectroscopic characterization of rice and corn starch. Microscopy Research and Technique. 2020 Jan 1. https://doi.org/10.1002/jemt.23437