Microtubule-associated protein 2, a marker of neuronal differentiation, induces mitotic defects, inhibits growth of melanoma cells, and predicts metastatic potential of cutaneous melanoma

Mohammad H. Soltani, Rita Pichardo, Ziqui Song, Namrata Sangha, Fabian Camacho, Kapaettu Satyamoorthy, Omar P. Sangueza, Vijayasaradhi Setaluri

    Research output: Contribution to journalArticle

    79 Citations (Scopus)

    Abstract

    Dynamic instability of microtubules is critical for mitotic spindle assembly and disassembly during cell division, especially in rapidly dividing tumor cells. Microtubule-associated proteins (MAPs) are a family of proteins that influence this property. We showed previously that MAP2, a neuron-specific protein that stabilizes microtubules in the dendrites of postmitotic neurons, is induced in primary cutaneous melanoma but is absent in metastatic melanomas. We proposed that induction of a microtubule-stabilizing protein in primary melanoma could disrupt the dynamic instability of microtubules, inhibit cell division and prevent or delay tumor progression. Here we show, by Kaplan-Meier survival and multivariate Cox regression analysis, that patients diagnosed with MAP2+ primary melanomas have significantly better metastatic disease-free survival than those with MAP2- disease. Investigation of the mechanisms that underlie the effect of MAP2 on melanoma progression showed that MAP2 expression in metastatic melanoma cell lines leads to microtubule stabilization, cell cycle arrest in G2-M phase and growth inhibition. Disruption of microtubule dynamics by MAP2 resulted in multipolar mitotic spindles, defects in cytokinesis and accumulation of cells with large nuclei, similar to those seen in vivo in MAP2+ primary melanomas cells. These data suggest that ectopic activation of a neuronal differentiation gene in melanoma during early tumor progression inhibits cell division and correlates with inhibition or delay of metastasis.

    Original languageEnglish
    Pages (from-to)1841-1850
    Number of pages10
    JournalAmerican Journal of Pathology
    Volume166
    Issue number6
    DOIs
    Publication statusPublished - 2005

    Fingerprint

    Microtubule-Associated Proteins
    Differentiation Antigens
    Melanoma
    Skin
    Growth
    Microtubules
    Cell Division
    Microtubule Proteins
    Spindle Apparatus
    Neurons
    Neoplasms
    Cytokinesis
    G2 Phase
    Dendrites
    Cell Cycle Checkpoints
    Disease-Free Survival
    Regression Analysis
    Neoplasm Metastasis
    Cell Line
    Survival

    All Science Journal Classification (ASJC) codes

    • Pathology and Forensic Medicine

    Cite this

    Soltani, Mohammad H. ; Pichardo, Rita ; Song, Ziqui ; Sangha, Namrata ; Camacho, Fabian ; Satyamoorthy, Kapaettu ; Sangueza, Omar P. ; Setaluri, Vijayasaradhi. / Microtubule-associated protein 2, a marker of neuronal differentiation, induces mitotic defects, inhibits growth of melanoma cells, and predicts metastatic potential of cutaneous melanoma. In: American Journal of Pathology. 2005 ; Vol. 166, No. 6. pp. 1841-1850.
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    Microtubule-associated protein 2, a marker of neuronal differentiation, induces mitotic defects, inhibits growth of melanoma cells, and predicts metastatic potential of cutaneous melanoma. / Soltani, Mohammad H.; Pichardo, Rita; Song, Ziqui; Sangha, Namrata; Camacho, Fabian; Satyamoorthy, Kapaettu; Sangueza, Omar P.; Setaluri, Vijayasaradhi.

    In: American Journal of Pathology, Vol. 166, No. 6, 2005, p. 1841-1850.

    Research output: Contribution to journalArticle

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    AU - Song, Ziqui

    AU - Sangha, Namrata

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    AU - Sangueza, Omar P.

    AU - Setaluri, Vijayasaradhi

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