Mini-αB-crystallin

A functional element of αB-crystallin with chaperone-like activity

Jaya Bhattacharyya, E. G.Padmanabha Udupa, Jing Wang, K. Krishna Sharma

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

α-Crystallin is a member of the family of small heat-shock proteins (sHSP) and is composed of two subunits, αA-crystallin and αB-crystallin, which exhibit molecular chaperone-like properties. In a previous study, we found that residues 70-88 in αA-crystallin can function like a molecular chaperone by preventing the aggregation and precipitation of denaturing substrate proteins [Sharma, K. K., et al. (2000) J. Biol. Chem. 275, 3767-3771]. In this study, we show that the complementary sequence in αB-crystallin, residues 73-92 (DRFSVNLDVKHFSPEELKVK), is the functional chaperone site of αB-crystallin. Like the mini-αA-crystallin chaperone, the mini-αB-crystallin chaperone interacts with 1,1′-bi(4-anilino)naphthalene-5,5′-disulphonic acid (bis-ANS) and also possesses significant β-sheet and random coil structure. Deletion of four residues (DRFS) from the N-terminus or deletion of C-terminus LKVK residues from the 73-92 peptide abolishes the chaperone-like activity against denaturing alcohol dehydrogenase. However, removal of DRFS or HFSPEELKVK is necessary to completely abolish the antiaggregation property of the peptide in insulin reduction assay. Substitution of Asp at a site corresponding to D80 in αB-crystallin with D-Asp or β-Asp results in a significant loss of chaperone-like activity. Kynurenine modification of His in the peptide abolishes the antiaggregation property of the mini-chaperone. These data suggest that the 73-92 region in αB-crystallin is one of the substrate binding sites during chaperone activity.

Original languageEnglish
Pages (from-to)3069-3076
Number of pages8
JournalBiochemistry
Volume45
Issue number9
DOIs
Publication statusPublished - 07-03-2006

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Crystallins
Molecular Chaperones
Peptides
Small Heat-Shock Proteins
Kynurenine
Alcohol Dehydrogenase
Substrates
Assays
Substitution reactions
Agglomeration
Binding Sites
Insulin

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Bhattacharyya, Jaya ; Udupa, E. G.Padmanabha ; Wang, Jing ; Sharma, K. Krishna. / Mini-αB-crystallin : A functional element of αB-crystallin with chaperone-like activity. In: Biochemistry. 2006 ; Vol. 45, No. 9. pp. 3069-3076.
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Mini-αB-crystallin : A functional element of αB-crystallin with chaperone-like activity. / Bhattacharyya, Jaya; Udupa, E. G.Padmanabha; Wang, Jing; Sharma, K. Krishna.

In: Biochemistry, Vol. 45, No. 9, 07.03.2006, p. 3069-3076.

Research output: Contribution to journalArticle

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