Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity

Sandhya Kumari, Prashantha Naik, B. L. Vishma, Sujith Raj Salian, Raviraj Anand Devkar, Saleemulla Khan, Srinivas Mutalik, Guruprasad Kalthur, Satish Kumar Adiga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A major drawback with cancer chemotherapy is its severe toxic effects on non-target tissues. Assessment of natural products for their protective effect against anticancer drugs-induced toxicity is gaining importance in cancer biology. The present study was aimed at assessing the protective effect of hydroethanolic extract of Indian propolis (HEIP) against mitomycin C (MMC)-induced genotoxicity and cytotoxicity. Swiss albino mice were injected with various doses of HEIP (100, 200, 300, 400, 600 and 800 mg/kg b. wt., i.p) 1 h prior to MMC (8 mg/kg, i.p.) injection. The geno- and cyto-toxicities were evaluated in mice by performing bone marrow micronucleus and TUNEL assays. In vitro antioxidant and lipid peroxidation inhibitory assays were carried out to understand the mechanism of the protective effects. The significant increase in the frequency of micronculeated cells (12.51 ± 0.48), apoptotic cells (23.43 ± 1.86) and reduction in P/N ratio (0.69 ± 0.04) compared with control indicated the potential geno- and cytotoxic effects of MMC in bone marrow. Pretreatment with HEIP resulted in the significant recovery of the toxic effects induced by MMC. HEIP at 400 mg/kg b. wt. was found to be the optimum dose imparting the maximum protective effects. The in vitro antioxidant and lipid peroxidation inhibitory assays suggest that the extract possesses substantial free radical scavenging activities. In conclusion, HEIP possesses substantial geno- and cyto-protective properties against MMC, which could be mediated through efficient free radical scavenging and inhibitory effect on lipid peroxidation.

Original languageEnglish
Pages (from-to)1789-1800
Number of pages12
JournalCytotechnology
Volume68
Issue number5
DOIs
Publication statusPublished - 01-10-2016

Fingerprint

Propolis
Mitomycin
Lipids
Toxicity
Assays
Bone
Bone Marrow
Scavenging
Cytotoxicity
Antioxidants
Free radicals
Lipid Peroxidation
Poisons
Chemotherapy
Free Radicals
Micronucleus Tests
Cells
Tissue
In Situ Nick-End Labeling
Recovery

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kumari, Sandhya ; Naik, Prashantha ; Vishma, B. L. ; Salian, Sujith Raj ; Devkar, Raviraj Anand ; Khan, Saleemulla ; Mutalik, Srinivas ; Kalthur, Guruprasad ; Adiga, Satish Kumar. / Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity. In: Cytotechnology. 2016 ; Vol. 68, No. 5. pp. 1789-1800.
@article{2d77cc3d903c4b45b9c4de3b51910e11,
title = "Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity",
abstract = "A major drawback with cancer chemotherapy is its severe toxic effects on non-target tissues. Assessment of natural products for their protective effect against anticancer drugs-induced toxicity is gaining importance in cancer biology. The present study was aimed at assessing the protective effect of hydroethanolic extract of Indian propolis (HEIP) against mitomycin C (MMC)-induced genotoxicity and cytotoxicity. Swiss albino mice were injected with various doses of HEIP (100, 200, 300, 400, 600 and 800 mg/kg b. wt., i.p) 1 h prior to MMC (8 mg/kg, i.p.) injection. The geno- and cyto-toxicities were evaluated in mice by performing bone marrow micronucleus and TUNEL assays. In vitro antioxidant and lipid peroxidation inhibitory assays were carried out to understand the mechanism of the protective effects. The significant increase in the frequency of micronculeated cells (12.51 ± 0.48), apoptotic cells (23.43 ± 1.86) and reduction in P/N ratio (0.69 ± 0.04) compared with control indicated the potential geno- and cytotoxic effects of MMC in bone marrow. Pretreatment with HEIP resulted in the significant recovery of the toxic effects induced by MMC. HEIP at 400 mg/kg b. wt. was found to be the optimum dose imparting the maximum protective effects. The in vitro antioxidant and lipid peroxidation inhibitory assays suggest that the extract possesses substantial free radical scavenging activities. In conclusion, HEIP possesses substantial geno- and cyto-protective properties against MMC, which could be mediated through efficient free radical scavenging and inhibitory effect on lipid peroxidation.",
author = "Sandhya Kumari and Prashantha Naik and Vishma, {B. L.} and Salian, {Sujith Raj} and Devkar, {Raviraj Anand} and Saleemulla Khan and Srinivas Mutalik and Guruprasad Kalthur and Adiga, {Satish Kumar}",
year = "2016",
month = "10",
day = "1",
doi = "10.1007/s10616-015-9931-4",
language = "English",
volume = "68",
pages = "1789--1800",
journal = "Cytotechnology",
issn = "0920-9069",
publisher = "Springer Netherlands",
number = "5",

}

Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity. / Kumari, Sandhya; Naik, Prashantha; Vishma, B. L.; Salian, Sujith Raj; Devkar, Raviraj Anand; Khan, Saleemulla; Mutalik, Srinivas; Kalthur, Guruprasad; Adiga, Satish Kumar.

In: Cytotechnology, Vol. 68, No. 5, 01.10.2016, p. 1789-1800.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity

AU - Kumari, Sandhya

AU - Naik, Prashantha

AU - Vishma, B. L.

AU - Salian, Sujith Raj

AU - Devkar, Raviraj Anand

AU - Khan, Saleemulla

AU - Mutalik, Srinivas

AU - Kalthur, Guruprasad

AU - Adiga, Satish Kumar

PY - 2016/10/1

Y1 - 2016/10/1

N2 - A major drawback with cancer chemotherapy is its severe toxic effects on non-target tissues. Assessment of natural products for their protective effect against anticancer drugs-induced toxicity is gaining importance in cancer biology. The present study was aimed at assessing the protective effect of hydroethanolic extract of Indian propolis (HEIP) against mitomycin C (MMC)-induced genotoxicity and cytotoxicity. Swiss albino mice were injected with various doses of HEIP (100, 200, 300, 400, 600 and 800 mg/kg b. wt., i.p) 1 h prior to MMC (8 mg/kg, i.p.) injection. The geno- and cyto-toxicities were evaluated in mice by performing bone marrow micronucleus and TUNEL assays. In vitro antioxidant and lipid peroxidation inhibitory assays were carried out to understand the mechanism of the protective effects. The significant increase in the frequency of micronculeated cells (12.51 ± 0.48), apoptotic cells (23.43 ± 1.86) and reduction in P/N ratio (0.69 ± 0.04) compared with control indicated the potential geno- and cytotoxic effects of MMC in bone marrow. Pretreatment with HEIP resulted in the significant recovery of the toxic effects induced by MMC. HEIP at 400 mg/kg b. wt. was found to be the optimum dose imparting the maximum protective effects. The in vitro antioxidant and lipid peroxidation inhibitory assays suggest that the extract possesses substantial free radical scavenging activities. In conclusion, HEIP possesses substantial geno- and cyto-protective properties against MMC, which could be mediated through efficient free radical scavenging and inhibitory effect on lipid peroxidation.

AB - A major drawback with cancer chemotherapy is its severe toxic effects on non-target tissues. Assessment of natural products for their protective effect against anticancer drugs-induced toxicity is gaining importance in cancer biology. The present study was aimed at assessing the protective effect of hydroethanolic extract of Indian propolis (HEIP) against mitomycin C (MMC)-induced genotoxicity and cytotoxicity. Swiss albino mice were injected with various doses of HEIP (100, 200, 300, 400, 600 and 800 mg/kg b. wt., i.p) 1 h prior to MMC (8 mg/kg, i.p.) injection. The geno- and cyto-toxicities were evaluated in mice by performing bone marrow micronucleus and TUNEL assays. In vitro antioxidant and lipid peroxidation inhibitory assays were carried out to understand the mechanism of the protective effects. The significant increase in the frequency of micronculeated cells (12.51 ± 0.48), apoptotic cells (23.43 ± 1.86) and reduction in P/N ratio (0.69 ± 0.04) compared with control indicated the potential geno- and cytotoxic effects of MMC in bone marrow. Pretreatment with HEIP resulted in the significant recovery of the toxic effects induced by MMC. HEIP at 400 mg/kg b. wt. was found to be the optimum dose imparting the maximum protective effects. The in vitro antioxidant and lipid peroxidation inhibitory assays suggest that the extract possesses substantial free radical scavenging activities. In conclusion, HEIP possesses substantial geno- and cyto-protective properties against MMC, which could be mediated through efficient free radical scavenging and inhibitory effect on lipid peroxidation.

UR - http://www.scopus.com/inward/record.url?scp=84947778278&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947778278&partnerID=8YFLogxK

U2 - 10.1007/s10616-015-9931-4

DO - 10.1007/s10616-015-9931-4

M3 - Article

AN - SCOPUS:84947778278

VL - 68

SP - 1789

EP - 1800

JO - Cytotechnology

JF - Cytotechnology

SN - 0920-9069

IS - 5

ER -