Abstract

This study was undertaken to investigate the mitochondrial DNA (mtDNA) variation in non-malignant and malignant cervical tissue samples. We have identified 229 and 739 variations non-malignant and malignant tissues respectively distributed over 321 locations in the D-loop (50 in non-malignant and 166 in malignant; 216 variations), coding region (139 in non-malignant and 455 in malignant; 594 variations) tRNA and rRNA genes (39 in non-malignant and 119 in malignant; 158 variations). Besides, 77 novel and 34 various other disease associated variations were identified in non-malignant and malignant samples. A total of 236 tumor specific variations in 201 locations representing 30.1% in D-loop, 59.3% in coding regions and 10.6% in RNA genes were also identified. Our study shows that D loop (in 67 locations) is highly altered followed by ND5 (35 locations) region. Moreover, mtDNA alterations were significantly higher in malignant samples by two tailed Fisher's exact test (P. ≤. 0.05) with decreased mtDNA copy numbers. Bioinformatic analysis of 59 non-synonymous changes predicted several variations as damaging leading to decreased stability of the proteins. Taken together, mtDNA is highly altered in cervical cancer and functional studies are needed to be investigated to understand the consequence of these variations in cervical carcinogenesis and their potential application as biomarkers.

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalMitochondrion
Volume16
DOIs
Publication statusPublished - 2014

Fingerprint

Mitochondrial DNA
Uterine Cervical Neoplasms
Protein Stability
Transfer RNA
Computational Biology
rRNA Genes
Carcinogenesis
Biomarkers
RNA
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Medicine(all)

Cite this

Kabekkodu, Shama Prasada ; Bhat, Samatha ; Mascarenhas, Roshan ; Mallya, Sandeep ; Bhat, Manoj ; Pandey, Deeksha ; Kushtagi, Pralhad ; Thangaraj, Kumarasamy ; Gopinath, P. M. ; Satyamoorthy, Kapaettu. / Mitochondrial DNA variation analysis in cervical cancer. In: Mitochondrion. 2014 ; Vol. 16. pp. 73-82.
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title = "Mitochondrial DNA variation analysis in cervical cancer",
abstract = "This study was undertaken to investigate the mitochondrial DNA (mtDNA) variation in non-malignant and malignant cervical tissue samples. We have identified 229 and 739 variations non-malignant and malignant tissues respectively distributed over 321 locations in the D-loop (50 in non-malignant and 166 in malignant; 216 variations), coding region (139 in non-malignant and 455 in malignant; 594 variations) tRNA and rRNA genes (39 in non-malignant and 119 in malignant; 158 variations). Besides, 77 novel and 34 various other disease associated variations were identified in non-malignant and malignant samples. A total of 236 tumor specific variations in 201 locations representing 30.1{\%} in D-loop, 59.3{\%} in coding regions and 10.6{\%} in RNA genes were also identified. Our study shows that D loop (in 67 locations) is highly altered followed by ND5 (35 locations) region. Moreover, mtDNA alterations were significantly higher in malignant samples by two tailed Fisher's exact test (P. ≤. 0.05) with decreased mtDNA copy numbers. Bioinformatic analysis of 59 non-synonymous changes predicted several variations as damaging leading to decreased stability of the proteins. Taken together, mtDNA is highly altered in cervical cancer and functional studies are needed to be investigated to understand the consequence of these variations in cervical carcinogenesis and their potential application as biomarkers.",
author = "Kabekkodu, {Shama Prasada} and Samatha Bhat and Roshan Mascarenhas and Sandeep Mallya and Manoj Bhat and Deeksha Pandey and Pralhad Kushtagi and Kumarasamy Thangaraj and Gopinath, {P. M.} and Kapaettu Satyamoorthy",
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Mitochondrial DNA variation analysis in cervical cancer. / Kabekkodu, Shama Prasada; Bhat, Samatha; Mascarenhas, Roshan; Mallya, Sandeep; Bhat, Manoj; Pandey, Deeksha; Kushtagi, Pralhad; Thangaraj, Kumarasamy; Gopinath, P. M.; Satyamoorthy, Kapaettu.

In: Mitochondrion, Vol. 16, 2014, p. 73-82.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mitochondrial DNA variation analysis in cervical cancer

AU - Kabekkodu, Shama Prasada

AU - Bhat, Samatha

AU - Mascarenhas, Roshan

AU - Mallya, Sandeep

AU - Bhat, Manoj

AU - Pandey, Deeksha

AU - Kushtagi, Pralhad

AU - Thangaraj, Kumarasamy

AU - Gopinath, P. M.

AU - Satyamoorthy, Kapaettu

PY - 2014

Y1 - 2014

N2 - This study was undertaken to investigate the mitochondrial DNA (mtDNA) variation in non-malignant and malignant cervical tissue samples. We have identified 229 and 739 variations non-malignant and malignant tissues respectively distributed over 321 locations in the D-loop (50 in non-malignant and 166 in malignant; 216 variations), coding region (139 in non-malignant and 455 in malignant; 594 variations) tRNA and rRNA genes (39 in non-malignant and 119 in malignant; 158 variations). Besides, 77 novel and 34 various other disease associated variations were identified in non-malignant and malignant samples. A total of 236 tumor specific variations in 201 locations representing 30.1% in D-loop, 59.3% in coding regions and 10.6% in RNA genes were also identified. Our study shows that D loop (in 67 locations) is highly altered followed by ND5 (35 locations) region. Moreover, mtDNA alterations were significantly higher in malignant samples by two tailed Fisher's exact test (P. ≤. 0.05) with decreased mtDNA copy numbers. Bioinformatic analysis of 59 non-synonymous changes predicted several variations as damaging leading to decreased stability of the proteins. Taken together, mtDNA is highly altered in cervical cancer and functional studies are needed to be investigated to understand the consequence of these variations in cervical carcinogenesis and their potential application as biomarkers.

AB - This study was undertaken to investigate the mitochondrial DNA (mtDNA) variation in non-malignant and malignant cervical tissue samples. We have identified 229 and 739 variations non-malignant and malignant tissues respectively distributed over 321 locations in the D-loop (50 in non-malignant and 166 in malignant; 216 variations), coding region (139 in non-malignant and 455 in malignant; 594 variations) tRNA and rRNA genes (39 in non-malignant and 119 in malignant; 158 variations). Besides, 77 novel and 34 various other disease associated variations were identified in non-malignant and malignant samples. A total of 236 tumor specific variations in 201 locations representing 30.1% in D-loop, 59.3% in coding regions and 10.6% in RNA genes were also identified. Our study shows that D loop (in 67 locations) is highly altered followed by ND5 (35 locations) region. Moreover, mtDNA alterations were significantly higher in malignant samples by two tailed Fisher's exact test (P. ≤. 0.05) with decreased mtDNA copy numbers. Bioinformatic analysis of 59 non-synonymous changes predicted several variations as damaging leading to decreased stability of the proteins. Taken together, mtDNA is highly altered in cervical cancer and functional studies are needed to be investigated to understand the consequence of these variations in cervical carcinogenesis and their potential application as biomarkers.

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