Abstract
Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating to renal failure in the field of research.
| Original language | English |
|---|---|
| Pages (from-to) | 401-403 |
| Number of pages | 3 |
| Journal | Indian Journal of Clinical Biochemistry |
| Volume | 23 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 09-12-2008 |
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All Science Journal Classification (ASJC) codes
- Clinical Biochemistry
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Modified uremic rat model - A tool for uremic studies in rats. / Iype, Merin; Upadhya, Subramanya; Upadhya, Sharmila; Bhat, Gopalakrishna.
In: Indian Journal of Clinical Biochemistry, Vol. 23, No. 4, 09.12.2008, p. 401-403.Research output: Contribution to journal › Article
TY - JOUR
T1 - Modified uremic rat model - A tool for uremic studies in rats
AU - Iype, Merin
AU - Upadhya, Subramanya
AU - Upadhya, Sharmila
AU - Bhat, Gopalakrishna
PY - 2008/12/9
Y1 - 2008/12/9
N2 - Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating to renal failure in the field of research.
AB - Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating to renal failure in the field of research.
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U2 - 10.1007/s12291-008-0088-1
DO - 10.1007/s12291-008-0088-1
M3 - Article
VL - 23
SP - 401
EP - 403
JO - Indian Journal of Clinical Biochemistry
JF - Indian Journal of Clinical Biochemistry
SN - 0970-1915
IS - 4
ER -