Modified uremic rat model - A tool for uremic studies in rats

Merin Iype, Subramanya Upadhya, Sharmila Upadhya, Gopalakrishna Bhat

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating to renal failure in the field of research.

Original languageEnglish
Pages (from-to)401-403
Number of pages3
JournalIndian Journal of Clinical Biochemistry
Volume23
Issue number4
DOIs
Publication statusPublished - 09-12-2008

Fingerprint

Uremia
Rats
Renal Insufficiency
Urea
Creatinine
Serum
Hypovolemia
Animals
Renal Artery
Chronic Kidney Failure
Ligation
Theoretical Models
Hemorrhage
Blood
Research

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Iype, Merin ; Upadhya, Subramanya ; Upadhya, Sharmila ; Bhat, Gopalakrishna. / Modified uremic rat model - A tool for uremic studies in rats. In: Indian Journal of Clinical Biochemistry. 2008 ; Vol. 23, No. 4. pp. 401-403.
@article{ae98bf9375c84fbbb815d45411937fc5,
title = "Modified uremic rat model - A tool for uremic studies in rats",
abstract = "Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating to renal failure in the field of research.",
author = "Merin Iype and Subramanya Upadhya and Sharmila Upadhya and Gopalakrishna Bhat",
year = "2008",
month = "12",
day = "9",
doi = "10.1007/s12291-008-0088-1",
language = "English",
volume = "23",
pages = "401--403",
journal = "Indian Journal of Clinical Biochemistry",
issn = "0970-1915",
publisher = "Springer India",
number = "4",

}

Modified uremic rat model - A tool for uremic studies in rats. / Iype, Merin; Upadhya, Subramanya; Upadhya, Sharmila; Bhat, Gopalakrishna.

In: Indian Journal of Clinical Biochemistry, Vol. 23, No. 4, 09.12.2008, p. 401-403.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Modified uremic rat model - A tool for uremic studies in rats

AU - Iype, Merin

AU - Upadhya, Subramanya

AU - Upadhya, Sharmila

AU - Bhat, Gopalakrishna

PY - 2008/12/9

Y1 - 2008/12/9

N2 - Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating to renal failure in the field of research.

AB - Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating to renal failure in the field of research.

UR - http://www.scopus.com/inward/record.url?scp=57149116785&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57149116785&partnerID=8YFLogxK

U2 - 10.1007/s12291-008-0088-1

DO - 10.1007/s12291-008-0088-1

M3 - Article

AN - SCOPUS:57149116785

VL - 23

SP - 401

EP - 403

JO - Indian Journal of Clinical Biochemistry

JF - Indian Journal of Clinical Biochemistry

SN - 0970-1915

IS - 4

ER -