Modulation of gamma ray-induced genotoxic effect by thymol, a monoterpene phenol derivative of cymene

P. R. Archana, B. Nageshwar Rao, B. S. Satish Rao

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The radioprotective effect of thymol (TOH), a monoterpene phenol, on radiation-induced DNA damage was analyzed in vitro. Chinese hamster lung fibroblast cells (V79) were treated with different concentrations of TOH (0-100 μg/mL) for 1 hour before exposure to 3 Gy gamma irradiation, and then cytokinesis-blocked micronucleus and single-cell gel electrophoresis (comet assay) assays were used to evaluate the radiation-induced cytogenetic damage and genotoxic effects. Furthermore, the modulating effect of TOH on radiation-induced cell death was assessed by apoptotic and necrotic cell detection by staining with ethidium bromide/acridine orange using fluorescence microscopy. To understand the mechanism of TOH-imparted cytoprotection, mitochondrial membrane potential (MMP) was detected by flow cytometry after staining the cells with Rhodamine 123. Pretreatment of V79 cells with various concentrations of TOH (0-100 μg/mL) for 1 hour reduced the radiation-induced micronuclei as well as percent tail DNA and mean Olive tail moment with a maximum protective effect observed at TOH (25 μg/mL). Apoptosis by microscopic, MMP measurements indicated that the V79 cells exposed to gamma radiation alone showed a maximal increase in the number of early and late apoptotic and necrotic cell death associated with a significant loss of the MMP. Pretreatment with TOH (25 μg/mL) showed a significant (P <.01) decrease in the level of apoptotic fraction as well as necrotic cells and suppressed the radiation-induced collapse of MMP when compared with the radiation alone group. These results suggest that TOH suppresses radiation-induced genotoxicity, apoptosis, and necrosis primarily by the free radical scavenging and modulation of oxidative stress.

Original languageEnglish
Pages (from-to)374-383
Number of pages10
JournalIntegrative Cancer Therapies
Volume10
Issue number4
DOIs
Publication statusPublished - 12-2011

Fingerprint

Thymol
Monoterpenes
Gamma Rays
Phenol
Radiation
Mitochondrial Membrane Potential
Comet Assay
Tail
Cell Death
Apoptosis
Staining and Labeling
Rhodamine 123
Acridine Orange
Cytokinesis
Cytoprotection
Ethidium
Olea
Cricetulus
Fluorescence Microscopy
Cytogenetics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Complementary and alternative medicine

Cite this

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abstract = "The radioprotective effect of thymol (TOH), a monoterpene phenol, on radiation-induced DNA damage was analyzed in vitro. Chinese hamster lung fibroblast cells (V79) were treated with different concentrations of TOH (0-100 μg/mL) for 1 hour before exposure to 3 Gy gamma irradiation, and then cytokinesis-blocked micronucleus and single-cell gel electrophoresis (comet assay) assays were used to evaluate the radiation-induced cytogenetic damage and genotoxic effects. Furthermore, the modulating effect of TOH on radiation-induced cell death was assessed by apoptotic and necrotic cell detection by staining with ethidium bromide/acridine orange using fluorescence microscopy. To understand the mechanism of TOH-imparted cytoprotection, mitochondrial membrane potential (MMP) was detected by flow cytometry after staining the cells with Rhodamine 123. Pretreatment of V79 cells with various concentrations of TOH (0-100 μg/mL) for 1 hour reduced the radiation-induced micronuclei as well as percent tail DNA and mean Olive tail moment with a maximum protective effect observed at TOH (25 μg/mL). Apoptosis by microscopic, MMP measurements indicated that the V79 cells exposed to gamma radiation alone showed a maximal increase in the number of early and late apoptotic and necrotic cell death associated with a significant loss of the MMP. Pretreatment with TOH (25 μg/mL) showed a significant (P <.01) decrease in the level of apoptotic fraction as well as necrotic cells and suppressed the radiation-induced collapse of MMP when compared with the radiation alone group. These results suggest that TOH suppresses radiation-induced genotoxicity, apoptosis, and necrosis primarily by the free radical scavenging and modulation of oxidative stress.",
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Modulation of gamma ray-induced genotoxic effect by thymol, a monoterpene phenol derivative of cymene. / Archana, P. R.; Nageshwar Rao, B.; Satish Rao, B. S.

In: Integrative Cancer Therapies, Vol. 10, No. 4, 12.2011, p. 374-383.

Research output: Contribution to journalArticle

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