Molecular characterisation of uropathogenic Escherichia coli isolates at a tertiary care hospital in South India

Arindam Chakraborty, Prabha Adhikari, Shalini Shenoy, Vishwas Saralaya

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Uropathogenic Escherichia coli (UPEC) express a multitude of virulence factors (VFs) to break the inertia of the mucosal barrier of the urinary tract. The aim of the present study was undertaken to characterised the UPEC strains and to correlate carriage of specific virulence markers with different phylogroups and also to correlate these findings with clinical outcome of patients. A total of 156 non-repeated, clinically significant UPEC isolates were studied. Virulent genes were determined by two set of multiplex polymerase chain reaction (PCR). Phylogenetic analysis was performed by triplex PCR methods. Antibiograms and patient's clinical outcomes were collected in a structured pro forma. Of the 156 patients infected by UPEC strains with significant bacterial counts the most common predisposing factors were diabetes (45.5%) followed by carcinoma (7%). On analysis of the VF genes of the isolates, a majority of strains (140; 90%) were possessing the fimH gene followed by iutA (98; 63%), papC (76; 49%), cnf1 (46; 29.5%), hlyA (45; 29%) and neuC (8; 5%), respectively. On phylogenetic analysis, 27 (17%) isolates were belong to phylogroup A, 16 (10%) strains to Group B1, 59 (38%) were from Group B2 and 54 (35%) were from Group D. High prevalence of antibiotic resistance was observed among the isolates. The incidence of papC, cnf1 and hlyA was significantly higher (P < 0.05) among the isolates from relapse patients. Our findings indicate that virulent as well as commensal strains are capable of causing urinary tract infection. Virulence genes as well as patients-related factors are equally responsible for the development of infections and also that virulence genes may help such isolates to persist even with appropriate chemotherapy and be responsible for recurrent infections.

Original languageEnglish
Pages (from-to)305-310
Number of pages6
JournalIndian Journal of Medical Microbiology
Volume35
Issue number2
DOIs
Publication statusPublished - 01-04-2017

Fingerprint

Uropathogenic Escherichia coli
Tertiary Healthcare
Tertiary Care Centers
India
Virulence
Genes
Multiplex Polymerase Chain Reaction
Virulence Factors
Bacterial Load
Microbial Sensitivity Tests
Microbial Drug Resistance
Infection
Urinary Tract
Urinary Tract Infections
Causality
Carcinoma
Recurrence
Drug Therapy
Incidence

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

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abstract = "Uropathogenic Escherichia coli (UPEC) express a multitude of virulence factors (VFs) to break the inertia of the mucosal barrier of the urinary tract. The aim of the present study was undertaken to characterised the UPEC strains and to correlate carriage of specific virulence markers with different phylogroups and also to correlate these findings with clinical outcome of patients. A total of 156 non-repeated, clinically significant UPEC isolates were studied. Virulent genes were determined by two set of multiplex polymerase chain reaction (PCR). Phylogenetic analysis was performed by triplex PCR methods. Antibiograms and patient's clinical outcomes were collected in a structured pro forma. Of the 156 patients infected by UPEC strains with significant bacterial counts the most common predisposing factors were diabetes (45.5{\%}) followed by carcinoma (7{\%}). On analysis of the VF genes of the isolates, a majority of strains (140; 90{\%}) were possessing the fimH gene followed by iutA (98; 63{\%}), papC (76; 49{\%}), cnf1 (46; 29.5{\%}), hlyA (45; 29{\%}) and neuC (8; 5{\%}), respectively. On phylogenetic analysis, 27 (17{\%}) isolates were belong to phylogroup A, 16 (10{\%}) strains to Group B1, 59 (38{\%}) were from Group B2 and 54 (35{\%}) were from Group D. High prevalence of antibiotic resistance was observed among the isolates. The incidence of papC, cnf1 and hlyA was significantly higher (P < 0.05) among the isolates from relapse patients. Our findings indicate that virulent as well as commensal strains are capable of causing urinary tract infection. Virulence genes as well as patients-related factors are equally responsible for the development of infections and also that virulence genes may help such isolates to persist even with appropriate chemotherapy and be responsible for recurrent infections.",
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Molecular characterisation of uropathogenic Escherichia coli isolates at a tertiary care hospital in South India. / Chakraborty, Arindam; Adhikari, Prabha; Shenoy, Shalini; Saralaya, Vishwas.

In: Indian Journal of Medical Microbiology, Vol. 35, No. 2, 01.04.2017, p. 305-310.

Research output: Contribution to journalArticle

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