Molecular cloning and characterization of a partial mitogen-activated protein kinase cDNA, CnMAPK1 induced in response to water stress in coconut

Bobby Paul, M. K. Rajesh, S. Naresh Kumar

    Research output: Contribution to journalReview article

    2 Citations (Scopus)

    Abstract

    Plants have developed a fine-tuned complex of stress responses, among which one of the earliest signaling pathways after perception of stimuli is the evolutionary conserved mitogen-activated protein kinase (MAPK) cascade. These cascades are major pathways by which extracellular signals are transduced into internal responses. In this study, oligonucleotide primers were designed based on conserved regions of plant MAPK sequences available in pubic databases. A cDNA clone of 467 bp encoding a MAPK (designated CnMAPK1) was isolated by RT-PCR from leaves of coconut plantlets subjected to water stress using polyethylene glycol (PEG) treatment. CnMAPK1 was significantly homologous to other plant MAPKs viz., MPK7 and MPK5 from Zea mays, SIPK and MAPK6 from Oryza sativa and FLRS from Triticum aestivum (93% identity). Coconut MAPK belongs to the Serine/Threonine Kinases (STKs), Plant TEY MAPK subfamily group A. Molecular modeling of the coconut MAPK was also undertaken.

    Original languageEnglish
    Pages (from-to)145-150
    Number of pages6
    JournalIndian Journal of Horticulture
    Volume69
    Issue number2
    Publication statusPublished - 06-2012

    Fingerprint

    coconuts
    mitogen-activated protein kinase
    molecular cloning
    water stress
    DNA primers
    polyethylene glycol
    threonine
    serine
    plantlets
    stress response
    Oryza sativa
    phosphotransferases (kinases)
    Triticum aestivum
    Zea mays
    reverse transcriptase polymerase chain reaction
    clones
    leaves

    All Science Journal Classification (ASJC) codes

    • Horticulture

    Cite this

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    abstract = "Plants have developed a fine-tuned complex of stress responses, among which one of the earliest signaling pathways after perception of stimuli is the evolutionary conserved mitogen-activated protein kinase (MAPK) cascade. These cascades are major pathways by which extracellular signals are transduced into internal responses. In this study, oligonucleotide primers were designed based on conserved regions of plant MAPK sequences available in pubic databases. A cDNA clone of 467 bp encoding a MAPK (designated CnMAPK1) was isolated by RT-PCR from leaves of coconut plantlets subjected to water stress using polyethylene glycol (PEG) treatment. CnMAPK1 was significantly homologous to other plant MAPKs viz., MPK7 and MPK5 from Zea mays, SIPK and MAPK6 from Oryza sativa and FLRS from Triticum aestivum (93{\%} identity). Coconut MAPK belongs to the Serine/Threonine Kinases (STKs), Plant TEY MAPK subfamily group A. Molecular modeling of the coconut MAPK was also undertaken.",
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    Molecular cloning and characterization of a partial mitogen-activated protein kinase cDNA, CnMAPK1 induced in response to water stress in coconut. / Paul, Bobby; Rajesh, M. K.; Naresh Kumar, S.

    In: Indian Journal of Horticulture, Vol. 69, No. 2, 06.2012, p. 145-150.

    Research output: Contribution to journalReview article

    TY - JOUR

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    AU - Rajesh, M. K.

    AU - Naresh Kumar, S.

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    AB - Plants have developed a fine-tuned complex of stress responses, among which one of the earliest signaling pathways after perception of stimuli is the evolutionary conserved mitogen-activated protein kinase (MAPK) cascade. These cascades are major pathways by which extracellular signals are transduced into internal responses. In this study, oligonucleotide primers were designed based on conserved regions of plant MAPK sequences available in pubic databases. A cDNA clone of 467 bp encoding a MAPK (designated CnMAPK1) was isolated by RT-PCR from leaves of coconut plantlets subjected to water stress using polyethylene glycol (PEG) treatment. CnMAPK1 was significantly homologous to other plant MAPKs viz., MPK7 and MPK5 from Zea mays, SIPK and MAPK6 from Oryza sativa and FLRS from Triticum aestivum (93% identity). Coconut MAPK belongs to the Serine/Threonine Kinases (STKs), Plant TEY MAPK subfamily group A. Molecular modeling of the coconut MAPK was also undertaken.

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