Mueller matrix signature in advanced fluorescence microscopy imaging

Nirmal Mazumder, Jianjun Qiu, Fu Jen Kao, Alberto Diaspro

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have demonstrated the measurement and characterization of the polarization properties of a fluorescence signal using four-channel photon counting based Stokes-Mueller polarization microscopy. Thus, Lu-Chipman decomposition was applied to extract the critical polarization properties such as depolarization, linear retardance and the optical rotation of collagen type I fiber. We observed the spatial distribution of anisotropic and helical molecules of collagen from the reconstructed 2D Mueller images based on the fluorescence signal in a pixel-by-pixel manner.

Original languageEnglish
Article number025301
JournalJournal of Optics (United Kingdom)
Volume19
Issue number2
DOIs
Publication statusPublished - 01-02-2017

Fingerprint

Fluorescence microscopy
signatures
collagens
Polarization
microscopy
Imaging techniques
Collagen
fluorescence
polarization
matrices
Pixels
Fluorescence
pixels
Optical rotation
Depolarization
Collagen Type I
depolarization
Spatial distribution
Microscopic examination
counting

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Mazumder, Nirmal ; Qiu, Jianjun ; Kao, Fu Jen ; Diaspro, Alberto. / Mueller matrix signature in advanced fluorescence microscopy imaging. In: Journal of Optics (United Kingdom). 2017 ; Vol. 19, No. 2.
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Mueller matrix signature in advanced fluorescence microscopy imaging. / Mazumder, Nirmal; Qiu, Jianjun; Kao, Fu Jen; Diaspro, Alberto.

In: Journal of Optics (United Kingdom), Vol. 19, No. 2, 025301, 01.02.2017.

Research output: Contribution to journalArticle

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