Multidrug resistant pathogenic bacterial biofilm inhibition by Lactobacillus plantarum exopolysaccharide

Pradeepa, Akshay D. Shetty, Koshi Matthews, Aswathi R. Hegde, B. Akshatha, Alvita Betilla Mathias, Srinivas Mutalik, S. M. Vidya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The purpose of the present study was to evaluate the antibiofilm activity of the exopolysaccharide (EPS) isolated from fish derived Lactobacillus plantarum. The polysaccharide was purified by column chromatography and characterized by using Fourier transform infrared spectroscopy (FTIR), 1H NMR, HPLC, scanning electron microscope (SEM) and zeta potential (ζ). The EPS consist of three major sugars such as galactose, ribose and fructose at 137.5, 441, 159.3 μg/mg respectively. The antibiofilm activity of the EPS was performed against multidrug resistant pathogenic bacteria. Effect of EPS on bacterial cell surface hydrophobicity, cell-cell interaction and cell-surface interaction was estimated to understand the interaction between bacterial membrane and polysaccharide. Further, antibiotic susceptibility of established biofilm in the presence of EPS was investigated using standard antibiotics. Atomic force microscopy was employed to observe the morphology of bacterial biofilms. The results indicated that, the EPS exhibited good antibiofilm activity against biofilm forming pathogenic bacteria. Direct interaction between the EPS and pathogenic cell surface was observed in this study that lead to the reduction of surface hydrophobicity, cell interaction and antibiotic resistance patterns. Hence, the EPS isolated from Lactobacillus plantarum could serve as an antibiofilm agent in medical and pharmaceutical applications.

Original languageEnglish
Pages (from-to)7-14
Number of pages8
JournalBioactive Carbohydrates and Dietary Fibre
Volume8
Issue number1
DOIs
Publication statusPublished - 01-07-2016

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Lactobacillus plantarum
exopolysaccharides
Biofilms
Cell Communication
biofilm
Hydrophobic and Hydrophilic Interactions
Hydrophobicity
Anti-Bacterial Agents
Bacterial Polysaccharides
Bacteria
Polysaccharides
Ribose
Atomic Force Microscopy
Fourier Transform Infrared Spectroscopy
Microbial Drug Resistance
Fructose
Galactose
Column chromatography
Chromatography
Fishes

All Science Journal Classification (ASJC) codes

  • Food Science
  • Biochemistry
  • Organic Chemistry

Cite this

Pradeepa ; Shetty, Akshay D. ; Matthews, Koshi ; Hegde, Aswathi R. ; Akshatha, B. ; Mathias, Alvita Betilla ; Mutalik, Srinivas ; Vidya, S. M. / Multidrug resistant pathogenic bacterial biofilm inhibition by Lactobacillus plantarum exopolysaccharide. In: Bioactive Carbohydrates and Dietary Fibre. 2016 ; Vol. 8, No. 1. pp. 7-14.
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Multidrug resistant pathogenic bacterial biofilm inhibition by Lactobacillus plantarum exopolysaccharide. / Pradeepa; Shetty, Akshay D.; Matthews, Koshi; Hegde, Aswathi R.; Akshatha, B.; Mathias, Alvita Betilla; Mutalik, Srinivas; Vidya, S. M.

In: Bioactive Carbohydrates and Dietary Fibre, Vol. 8, No. 1, 01.07.2016, p. 7-14.

Research output: Contribution to journalArticle

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