TY - JOUR
T1 - Native and non-native host assessment towards metabolic pathway reconstructions of plant natural products
AU - Pujari, Ipsita
AU - Thomas, Abitha
AU - Sankar Babu, Vidhu
N1 - Funding Information:
The authors thank the Science and Engineering Research Board - Extra Mural Research (SERB-EMR) (presently called Core Research Grant [CRG]), Government of India, File No. EMR/2015/001816 for funding the research project. I.P. and A.T. thank Manipal Academy of Higher Education, Manipal, India, for providing the prestigious Dr T. M. A. Pai PhD Scholarship. I.P. acknowledges SERB for granting the Junior Research Fellowship from May 2017 to December 2019.
Funding Information:
The authors thank the Science and Engineering Research Board - Extra Mural Research (SERB-EMR) (presently called Core Research Grant [CRG]), Government of India , File No. EMR/2015/001816 for funding the research project. I.P. and A.T. thank Manipal Academy of Higher Education, Manipal, India , for providing the prestigious Dr T. M. A. Pai PhD Scholarship. I.P. acknowledges SERB for granting the Junior Research Fellowship from May 2017 to December 2019.
Publisher Copyright:
© 2021
PY - 2021/6
Y1 - 2021/6
N2 - Plant-based biopreparations are reasonably priced and are devoid of viral, prion and endotoxin contaminants. However, synthesizing these natural plant products by chemical methods is quite expensive. The structural complexity of plant-derived natural products poses a challenge for chemical synthesis at a commercial scale. Failure of commercial-scale synthesis is the chief reason why metabolic reconstructions in heterologous hosts are inevitable. This review discusses plant metabolite pathway reconstructions experimented in various heterologous hosts, and the inherent challenges involved. Plants as native hosts possess enhanced post-translational modification ability, along with rigorous gene edits, unlike microbes. To achieve a high yield of metabolites in plants, increased cell division rate is one of the requisites. This improved cell division rate will promote cellular homogeneity. Incorporation and maintenance of plant cell synchrony, in turn, can program stable product scale-up.
AB - Plant-based biopreparations are reasonably priced and are devoid of viral, prion and endotoxin contaminants. However, synthesizing these natural plant products by chemical methods is quite expensive. The structural complexity of plant-derived natural products poses a challenge for chemical synthesis at a commercial scale. Failure of commercial-scale synthesis is the chief reason why metabolic reconstructions in heterologous hosts are inevitable. This review discusses plant metabolite pathway reconstructions experimented in various heterologous hosts, and the inherent challenges involved. Plants as native hosts possess enhanced post-translational modification ability, along with rigorous gene edits, unlike microbes. To achieve a high yield of metabolites in plants, increased cell division rate is one of the requisites. This improved cell division rate will promote cellular homogeneity. Incorporation and maintenance of plant cell synchrony, in turn, can program stable product scale-up.
UR - http://www.scopus.com/inward/record.url?scp=85104390364&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85104390364&partnerID=8YFLogxK
U2 - 10.1016/j.btre.2021.e00619
DO - 10.1016/j.btre.2021.e00619
M3 - Review article
AN - SCOPUS:85104390364
SN - 2215-017X
VL - 30
JO - Biotechnology Reports
JF - Biotechnology Reports
M1 - e00619
ER -