Nephroprotective effect of aqueous extract of pimpinella anisum in gentamicin induced nephrotoxicity in wistar rats

Nair Aiswarya, Rao R. Rashmi, Shenoy J. Preethi, Vinod Chandran, S. Teerthanath, Pai B. Sunil, K. B. Rakesh, Nair Aiswarya, Rao R. Rashmi, Shenoy J. Preethi, Vinod Chandran, S. Teerthanath, Pai B. Sunil, K. B. Rakesh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Pimpinella anisum known for its various medicinal properties is also a natural antioxidant and a free radical scavenger with no documented evidence as a nephroprotective agent. Objective: To evaluate the nephroprotective activity of aqueous extract of Pimpinella anisum seeds in a rodent model of gentamicin induced nephrotoxicity. Materials and Methods: Wistar albino rats of either sex, weighing 150–200 g was divided into 5 groups; normal saline, gentamicin 80mg/kg, intraperitoneally for 8 days, aqueous extract of Pimpinella anisum seeds at 1, 2, and 4g/kg, per oral for 8 days, the test extract administered 3 days prior and concurrently with gentamicin for 5 days. Blood urea, serum creatinine, uric acid and blood urea nitrogen analyses and microscopic examination of kidney were performed. Results: Gentamicin treatment caused nephrotoxicity as evidenced by marked elevation in serum urea, serum uric acid, serum creatinine and blood urea nitrogen (107.5±16.92mg/dl, 0.8±0.09 mg/dl, 3.05±0.29 mg/dl, 47.8±9.07 mg/dl) respectively when compared to the saline treated groups. Co-administration of Pimpinella anisum extract with gentamicin decreased the rise in these parameters in a dose dependent manner. Histopathological analysis revealed epithelial loss with intense granular degeneration in gentamicin treated rats, whereas aqueous extract of Pimpinella anisum mitigated the severity of gentamicin-induced renal damage. Conclusion: To conclude, our data suggest that aqueous extract of Pimpinella anisum exhibits reno-protective effect in gentamicin induced renal damage and further studies on its mechanism of action are warranted.

Original languageEnglish
Pages (from-to)403-407
Number of pages5
JournalPharmacognosy Journal
Volume10
Issue number3
DOIs
Publication statusPublished - 01-05-2018
Externally publishedYes

Fingerprint

Pimpinella
Gentamicins
Wistar Rats
Blood Urea Nitrogen
Uric Acid
Serum
Kidney
Urea
Creatinine
Seeds
Free Radical Scavengers
Rodentia
Antioxidants

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

Aiswarya, N., Rashmi, R. R., Preethi, S. J., Chandran, V., Teerthanath, S., Sunil, P. B., ... Rakesh, K. B. (2018). Nephroprotective effect of aqueous extract of pimpinella anisum in gentamicin induced nephrotoxicity in wistar rats. Pharmacognosy Journal, 10(3), 403-407. https://doi.org/10.5530/pj.2018.3.66
Aiswarya, Nair ; Rashmi, Rao R. ; Preethi, Shenoy J. ; Chandran, Vinod ; Teerthanath, S. ; Sunil, Pai B. ; Rakesh, K. B. ; Aiswarya, Nair ; Rashmi, Rao R. ; Preethi, Shenoy J. ; Chandran, Vinod ; Teerthanath, S. ; Sunil, Pai B. ; Rakesh, K. B. / Nephroprotective effect of aqueous extract of pimpinella anisum in gentamicin induced nephrotoxicity in wistar rats. In: Pharmacognosy Journal. 2018 ; Vol. 10, No. 3. pp. 403-407.
@article{5067dc21448b48d3b7d5cafe17ea9213,
title = "Nephroprotective effect of aqueous extract of pimpinella anisum in gentamicin induced nephrotoxicity in wistar rats",
abstract = "Background: Pimpinella anisum known for its various medicinal properties is also a natural antioxidant and a free radical scavenger with no documented evidence as a nephroprotective agent. Objective: To evaluate the nephroprotective activity of aqueous extract of Pimpinella anisum seeds in a rodent model of gentamicin induced nephrotoxicity. Materials and Methods: Wistar albino rats of either sex, weighing 150–200 g was divided into 5 groups; normal saline, gentamicin 80mg/kg, intraperitoneally for 8 days, aqueous extract of Pimpinella anisum seeds at 1, 2, and 4g/kg, per oral for 8 days, the test extract administered 3 days prior and concurrently with gentamicin for 5 days. Blood urea, serum creatinine, uric acid and blood urea nitrogen analyses and microscopic examination of kidney were performed. Results: Gentamicin treatment caused nephrotoxicity as evidenced by marked elevation in serum urea, serum uric acid, serum creatinine and blood urea nitrogen (107.5±16.92mg/dl, 0.8±0.09 mg/dl, 3.05±0.29 mg/dl, 47.8±9.07 mg/dl) respectively when compared to the saline treated groups. Co-administration of Pimpinella anisum extract with gentamicin decreased the rise in these parameters in a dose dependent manner. Histopathological analysis revealed epithelial loss with intense granular degeneration in gentamicin treated rats, whereas aqueous extract of Pimpinella anisum mitigated the severity of gentamicin-induced renal damage. Conclusion: To conclude, our data suggest that aqueous extract of Pimpinella anisum exhibits reno-protective effect in gentamicin induced renal damage and further studies on its mechanism of action are warranted.",
author = "Nair Aiswarya and Rashmi, {Rao R.} and Preethi, {Shenoy J.} and Vinod Chandran and S. Teerthanath and Sunil, {Pai B.} and Rakesh, {K. B.} and Nair Aiswarya and Rashmi, {Rao R.} and Preethi, {Shenoy J.} and Vinod Chandran and S. Teerthanath and Sunil, {Pai B.} and Rakesh, {K. B.}",
year = "2018",
month = "5",
day = "1",
doi = "10.5530/pj.2018.3.66",
language = "English",
volume = "10",
pages = "403--407",
journal = "Pharmacognosy Journal",
issn = "0975-3575",
publisher = "Pharmacognosy Network Worldwide",
number = "3",

}

Aiswarya, N, Rashmi, RR, Preethi, SJ, Chandran, V, Teerthanath, S, Sunil, PB, Rakesh, KB, Aiswarya, N, Rashmi, RR, Preethi, SJ, Chandran, V, Teerthanath, S, Sunil, PB & Rakesh, KB 2018, 'Nephroprotective effect of aqueous extract of pimpinella anisum in gentamicin induced nephrotoxicity in wistar rats', Pharmacognosy Journal, vol. 10, no. 3, pp. 403-407. https://doi.org/10.5530/pj.2018.3.66

Nephroprotective effect of aqueous extract of pimpinella anisum in gentamicin induced nephrotoxicity in wistar rats. / Aiswarya, Nair; Rashmi, Rao R.; Preethi, Shenoy J.; Chandran, Vinod; Teerthanath, S.; Sunil, Pai B.; Rakesh, K. B.; Aiswarya, Nair; Rashmi, Rao R.; Preethi, Shenoy J.; Chandran, Vinod; Teerthanath, S.; Sunil, Pai B.; Rakesh, K. B.

In: Pharmacognosy Journal, Vol. 10, No. 3, 01.05.2018, p. 403-407.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nephroprotective effect of aqueous extract of pimpinella anisum in gentamicin induced nephrotoxicity in wistar rats

AU - Aiswarya, Nair

AU - Rashmi, Rao R.

AU - Preethi, Shenoy J.

AU - Chandran, Vinod

AU - Teerthanath, S.

AU - Sunil, Pai B.

AU - Rakesh, K. B.

AU - Aiswarya, Nair

AU - Rashmi, Rao R.

AU - Preethi, Shenoy J.

AU - Chandran, Vinod

AU - Teerthanath, S.

AU - Sunil, Pai B.

AU - Rakesh, K. B.

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Background: Pimpinella anisum known for its various medicinal properties is also a natural antioxidant and a free radical scavenger with no documented evidence as a nephroprotective agent. Objective: To evaluate the nephroprotective activity of aqueous extract of Pimpinella anisum seeds in a rodent model of gentamicin induced nephrotoxicity. Materials and Methods: Wistar albino rats of either sex, weighing 150–200 g was divided into 5 groups; normal saline, gentamicin 80mg/kg, intraperitoneally for 8 days, aqueous extract of Pimpinella anisum seeds at 1, 2, and 4g/kg, per oral for 8 days, the test extract administered 3 days prior and concurrently with gentamicin for 5 days. Blood urea, serum creatinine, uric acid and blood urea nitrogen analyses and microscopic examination of kidney were performed. Results: Gentamicin treatment caused nephrotoxicity as evidenced by marked elevation in serum urea, serum uric acid, serum creatinine and blood urea nitrogen (107.5±16.92mg/dl, 0.8±0.09 mg/dl, 3.05±0.29 mg/dl, 47.8±9.07 mg/dl) respectively when compared to the saline treated groups. Co-administration of Pimpinella anisum extract with gentamicin decreased the rise in these parameters in a dose dependent manner. Histopathological analysis revealed epithelial loss with intense granular degeneration in gentamicin treated rats, whereas aqueous extract of Pimpinella anisum mitigated the severity of gentamicin-induced renal damage. Conclusion: To conclude, our data suggest that aqueous extract of Pimpinella anisum exhibits reno-protective effect in gentamicin induced renal damage and further studies on its mechanism of action are warranted.

AB - Background: Pimpinella anisum known for its various medicinal properties is also a natural antioxidant and a free radical scavenger with no documented evidence as a nephroprotective agent. Objective: To evaluate the nephroprotective activity of aqueous extract of Pimpinella anisum seeds in a rodent model of gentamicin induced nephrotoxicity. Materials and Methods: Wistar albino rats of either sex, weighing 150–200 g was divided into 5 groups; normal saline, gentamicin 80mg/kg, intraperitoneally for 8 days, aqueous extract of Pimpinella anisum seeds at 1, 2, and 4g/kg, per oral for 8 days, the test extract administered 3 days prior and concurrently with gentamicin for 5 days. Blood urea, serum creatinine, uric acid and blood urea nitrogen analyses and microscopic examination of kidney were performed. Results: Gentamicin treatment caused nephrotoxicity as evidenced by marked elevation in serum urea, serum uric acid, serum creatinine and blood urea nitrogen (107.5±16.92mg/dl, 0.8±0.09 mg/dl, 3.05±0.29 mg/dl, 47.8±9.07 mg/dl) respectively when compared to the saline treated groups. Co-administration of Pimpinella anisum extract with gentamicin decreased the rise in these parameters in a dose dependent manner. Histopathological analysis revealed epithelial loss with intense granular degeneration in gentamicin treated rats, whereas aqueous extract of Pimpinella anisum mitigated the severity of gentamicin-induced renal damage. Conclusion: To conclude, our data suggest that aqueous extract of Pimpinella anisum exhibits reno-protective effect in gentamicin induced renal damage and further studies on its mechanism of action are warranted.

UR - http://www.scopus.com/inward/record.url?scp=85052021689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052021689&partnerID=8YFLogxK

U2 - 10.5530/pj.2018.3.66

DO - 10.5530/pj.2018.3.66

M3 - Article

VL - 10

SP - 403

EP - 407

JO - Pharmacognosy Journal

JF - Pharmacognosy Journal

SN - 0975-3575

IS - 3

ER -