Neural stem cell or human induced pluripotent stem cell–derived gaba-ergic progenitor cell grafting in an animal model of chronic temporal lobe epilepsy

Dinesh Upadhya, Bharathi Hattiangady, Geetha A. Shetty, Gabriele Zanirati, Maheedhar Kodali, Ashok K. Shetty

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Grafting of neural stem cells (NSCs) or GABA-ergic progenitor cells (GPCs) into the hippocampus could offer an alternative therapy to hippocampal resection in patients with drug-resistant chronic epilepsy, which afflicts >30% of temporal lobe epilepsy (TLE) cases. Multipotent, self-renewing NSCs could be expanded from multiple regions of the developing and adult brain, human embryonic stem cells (hESCs), and human induced pluripotent stem cells (hiP-SCs). On the other hand, GPCs could be generated from the medial and lateral ganglionic eminences of the embryonic brain and from hESCs and hiPSCs. To provide comprehensive methodologies involved in testing the efficacy of transplantation of NSCs and GPCs in a rat model of chronic TLE, NSCs derived from the rat medial ganglionic eminence (MGE) and MGE-like GPCs derived from hiPSCs are taken as examples in this unit. The topics comprise description of the required materials, reagents and equipment, methods for obtaining rat MGE-NSCs and hiPSC-derived MGE-like GPCs in culture, generation of chronically epileptic rats, intrahippocampal grafting procedure, post-grafting evaluation of the effects of grafts on spontaneous recurrent seizures and cognitive and mood impairments, analyses of the yield and the fate of graft-derived cells, and the effects of grafts on the host hippocampus.

Original languageEnglish
JournalCurrent Protocols in Stem Cell Biology
Volume2016
DOIs
Publication statusPublished - 01-08-2016

Fingerprint

Neural Stem Cells
Temporal Lobe Epilepsy
Median Eminence
gamma-Aminobutyric Acid
Stem Cells
Animal Models
Induced Pluripotent Stem Cells
Transplants
Hippocampus
Brain
Complementary Therapies
Seizures
Cell Culture Techniques
Transplantation
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

@article{886da8206dd7458e80cb99c3ee37c3d2,
title = "Neural stem cell or human induced pluripotent stem cell–derived gaba-ergic progenitor cell grafting in an animal model of chronic temporal lobe epilepsy",
abstract = "Grafting of neural stem cells (NSCs) or GABA-ergic progenitor cells (GPCs) into the hippocampus could offer an alternative therapy to hippocampal resection in patients with drug-resistant chronic epilepsy, which afflicts >30{\%} of temporal lobe epilepsy (TLE) cases. Multipotent, self-renewing NSCs could be expanded from multiple regions of the developing and adult brain, human embryonic stem cells (hESCs), and human induced pluripotent stem cells (hiP-SCs). On the other hand, GPCs could be generated from the medial and lateral ganglionic eminences of the embryonic brain and from hESCs and hiPSCs. To provide comprehensive methodologies involved in testing the efficacy of transplantation of NSCs and GPCs in a rat model of chronic TLE, NSCs derived from the rat medial ganglionic eminence (MGE) and MGE-like GPCs derived from hiPSCs are taken as examples in this unit. The topics comprise description of the required materials, reagents and equipment, methods for obtaining rat MGE-NSCs and hiPSC-derived MGE-like GPCs in culture, generation of chronically epileptic rats, intrahippocampal grafting procedure, post-grafting evaluation of the effects of grafts on spontaneous recurrent seizures and cognitive and mood impairments, analyses of the yield and the fate of graft-derived cells, and the effects of grafts on the host hippocampus.",
author = "Dinesh Upadhya and Bharathi Hattiangady and Shetty, {Geetha A.} and Gabriele Zanirati and Maheedhar Kodali and Shetty, {Ashok K.}",
year = "2016",
month = "8",
day = "1",
doi = "10.1002/cpsc.9",
language = "English",
volume = "2016",
journal = "Current Protocols in Stem Cell Biology",
issn = "1941-7322",
publisher = "John Wiley and Sons Inc.",

}

Neural stem cell or human induced pluripotent stem cell–derived gaba-ergic progenitor cell grafting in an animal model of chronic temporal lobe epilepsy. / Upadhya, Dinesh; Hattiangady, Bharathi; Shetty, Geetha A.; Zanirati, Gabriele; Kodali, Maheedhar; Shetty, Ashok K.

In: Current Protocols in Stem Cell Biology, Vol. 2016, 01.08.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Neural stem cell or human induced pluripotent stem cell–derived gaba-ergic progenitor cell grafting in an animal model of chronic temporal lobe epilepsy

AU - Upadhya, Dinesh

AU - Hattiangady, Bharathi

AU - Shetty, Geetha A.

AU - Zanirati, Gabriele

AU - Kodali, Maheedhar

AU - Shetty, Ashok K.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Grafting of neural stem cells (NSCs) or GABA-ergic progenitor cells (GPCs) into the hippocampus could offer an alternative therapy to hippocampal resection in patients with drug-resistant chronic epilepsy, which afflicts >30% of temporal lobe epilepsy (TLE) cases. Multipotent, self-renewing NSCs could be expanded from multiple regions of the developing and adult brain, human embryonic stem cells (hESCs), and human induced pluripotent stem cells (hiP-SCs). On the other hand, GPCs could be generated from the medial and lateral ganglionic eminences of the embryonic brain and from hESCs and hiPSCs. To provide comprehensive methodologies involved in testing the efficacy of transplantation of NSCs and GPCs in a rat model of chronic TLE, NSCs derived from the rat medial ganglionic eminence (MGE) and MGE-like GPCs derived from hiPSCs are taken as examples in this unit. The topics comprise description of the required materials, reagents and equipment, methods for obtaining rat MGE-NSCs and hiPSC-derived MGE-like GPCs in culture, generation of chronically epileptic rats, intrahippocampal grafting procedure, post-grafting evaluation of the effects of grafts on spontaneous recurrent seizures and cognitive and mood impairments, analyses of the yield and the fate of graft-derived cells, and the effects of grafts on the host hippocampus.

AB - Grafting of neural stem cells (NSCs) or GABA-ergic progenitor cells (GPCs) into the hippocampus could offer an alternative therapy to hippocampal resection in patients with drug-resistant chronic epilepsy, which afflicts >30% of temporal lobe epilepsy (TLE) cases. Multipotent, self-renewing NSCs could be expanded from multiple regions of the developing and adult brain, human embryonic stem cells (hESCs), and human induced pluripotent stem cells (hiP-SCs). On the other hand, GPCs could be generated from the medial and lateral ganglionic eminences of the embryonic brain and from hESCs and hiPSCs. To provide comprehensive methodologies involved in testing the efficacy of transplantation of NSCs and GPCs in a rat model of chronic TLE, NSCs derived from the rat medial ganglionic eminence (MGE) and MGE-like GPCs derived from hiPSCs are taken as examples in this unit. The topics comprise description of the required materials, reagents and equipment, methods for obtaining rat MGE-NSCs and hiPSC-derived MGE-like GPCs in culture, generation of chronically epileptic rats, intrahippocampal grafting procedure, post-grafting evaluation of the effects of grafts on spontaneous recurrent seizures and cognitive and mood impairments, analyses of the yield and the fate of graft-derived cells, and the effects of grafts on the host hippocampus.

UR - http://www.scopus.com/inward/record.url?scp=85026664606&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026664606&partnerID=8YFLogxK

U2 - 10.1002/cpsc.9

DO - 10.1002/cpsc.9

M3 - Article

VL - 2016

JO - Current Protocols in Stem Cell Biology

JF - Current Protocols in Stem Cell Biology

SN - 1941-7322

ER -