Neuroimmune consequences of teratological insult induced by cyclophosphamide exposure during intrauterine life in mice

Prakash, Gajendra Singh, Sukhmahendra Singh

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Present work is aimed to establish central nervous system and immune system changes in newly born murine pups produced after cyclophosphamide exposure during intrauterine life. Cyclophosphamide in single dose of 10 or 20 mg/kg body weight was administered to pregnant mice on day 11 of gestation. Fetuses were dissected out on day 19 and studied for gross malformations. Brains and thymuses were collected for further investigations. Cyclophosphamide treated brains and thymuses were smaller in size and distorted in shape as compared to control brains and thymuses. Protein estimation of brains revealed decline in both quantitative and qualitative aspect. Study of brain cells and thymocytes revealed 1) increase in apoptotic cell count and 2) increase in percent DNA fragmentation. In vitro cell proliferation assay exhibited decrease in both brain cells and thymocytes. Similar pattern of response shown by brain cells and thymocytes conclude interaction between central nervous system and immune system insults produced by cyclophosphamide. Loss of certain neuropeptides has culminated in thymic compromise besides direct actions.

Original languageEnglish
Pages (from-to)6-11
Number of pages6
Publication statusPublished - 01-12-2007
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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