Neuroimmune consequences of teratological insult induced by cyclophosphamide exposure during intrauterine life in mice

Prakash, Gajendra Singh, Sukhmahendra Singh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Present work is aimed to establish central nervous system and immune system changes in newly born murine pups produced after cyclophosphamide exposure during intrauterine life. Cyclophosphamide in single dose of 10 or 20 mg/kg body weight was administered to pregnant mice on day 11 of gestation. Fetuses were dissected out on day 19 and studied for gross malformations. Brains and thymuses were collected for further investigations. Cyclophosphamide treated brains and thymuses were smaller in size and distorted in shape as compared to control brains and thymuses. Protein estimation of brains revealed decline in both quantitative and qualitative aspect. Study of brain cells and thymocytes revealed 1) increase in apoptotic cell count and 2) increase in percent DNA fragmentation. In vitro cell proliferation assay exhibited decrease in both brain cells and thymocytes. Similar pattern of response shown by brain cells and thymocytes conclude interaction between central nervous system and immune system insults produced by cyclophosphamide. Loss of certain neuropeptides has culminated in thymic compromise besides direct actions.

Original languageEnglish
Pages (from-to)6-11
Number of pages6
JournalNeuroanatomy
Volume6
Publication statusPublished - 01-12-2007
Externally publishedYes

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Cyclophosphamide
Brain
Thymocytes
Thymus Gland
Immune System
Central Nervous System
DNA Fragmentation
Neuropeptides
Fetus
Cell Count
Body Weight
Cell Proliferation
Pregnancy
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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Neuroimmune consequences of teratological insult induced by cyclophosphamide exposure during intrauterine life in mice. / Prakash; Singh, Gajendra; Singh, Sukhmahendra.

In: Neuroanatomy, Vol. 6, 01.12.2007, p. 6-11.

Research output: Contribution to journalArticle

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