Non-invasive, in vivo fluorescence technique as an objective tool for monitoring wound healing following Low Level Laser Therapy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Collagen represents major protein component of the extracellular matrix (ECM), thus its monitoring could be used as an ideal tool for assessing wound healing following therapy. Presently, there is a great need to develop quick, objective, non-destructive method to monitor collagen synthesis during progression of wound healing. The applicability of the Laser induced fluorescence technique towards wound healing monitoring by measuring collagen levels at different stages of the healing progression is the main idea behind the current work. Six to eight weeks old Swiss albino mice with ccircular wounds of 15 mm diameter were illuminated with single exposure of 2 J/cm2 from He-Ne laser (632.8 nm; 7 mW power; 4.02 mW/cm2 power density) along with un-illuminated and un-wounded controls. Spectroscopic changes were monitored by recording in vivo fluorescence from each animal (under anesthesia) at different post-wounding days (5th, 10th, 30 thday) by exciting granulation tissue/skin with 325 nm He-Cd laser. The autofluorescence from the tissue/skin was recorded from four different sites and four spectra were recorded from each site. A total of 2160 spectra were recorded from 45 animals. The in vivo fluorescence studies have shown significant increase (P<0.001) in collagen synthesis upon treatment with optimum laser dose of 2 J/cm2 immediately after wounding as compared to un-illuminated control group. It can conclude that in vivo fluorescence measurement is effective in monitoring wound healing and hence could be used over ex vivo method as an objective and non-intrusive method to monitor collagen levels.

Original languageEnglish
Title of host publicationPhotonic Therapeutics and Diagnostics IX
Volume8565
DOIs
Publication statusPublished - 2013
EventPhotonic Therapeutics and Diagnostics XI - San Francisco, United States
Duration: 07-02-201508-02-2015

Conference

ConferencePhotonic Therapeutics and Diagnostics XI
CountryUnited States
CitySan Francisco
Period07-02-1508-02-15

Fingerprint

wound healing
collagens
Collagen
Wound Healing
therapy
Fluorescence
Lasers
fluorescence
Monitoring
lasers
progressions
animals
Skin
Animals
Tissue
anesthesia
Granulation
Granulation Tissue
Extracellular Matrix Proteins
healing

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

@inproceedings{526492263301444786ea957485889bc8,
title = "Non-invasive, in vivo fluorescence technique as an objective tool for monitoring wound healing following Low Level Laser Therapy",
abstract = "Collagen represents major protein component of the extracellular matrix (ECM), thus its monitoring could be used as an ideal tool for assessing wound healing following therapy. Presently, there is a great need to develop quick, objective, non-destructive method to monitor collagen synthesis during progression of wound healing. The applicability of the Laser induced fluorescence technique towards wound healing monitoring by measuring collagen levels at different stages of the healing progression is the main idea behind the current work. Six to eight weeks old Swiss albino mice with ccircular wounds of 15 mm diameter were illuminated with single exposure of 2 J/cm2 from He-Ne laser (632.8 nm; 7 mW power; 4.02 mW/cm2 power density) along with un-illuminated and un-wounded controls. Spectroscopic changes were monitored by recording in vivo fluorescence from each animal (under anesthesia) at different post-wounding days (5th, 10th, 30 thday) by exciting granulation tissue/skin with 325 nm He-Cd laser. The autofluorescence from the tissue/skin was recorded from four different sites and four spectra were recorded from each site. A total of 2160 spectra were recorded from 45 animals. The in vivo fluorescence studies have shown significant increase (P<0.001) in collagen synthesis upon treatment with optimum laser dose of 2 J/cm2 immediately after wounding as compared to un-illuminated control group. It can conclude that in vivo fluorescence measurement is effective in monitoring wound healing and hence could be used over ex vivo method as an objective and non-intrusive method to monitor collagen levels.",
author = "Vijendra Prabhu and Fernandes, {Edward Mark} and Rao, {Bola Sadashiva Satish} and Mahato, {Krishna Kishore}",
year = "2013",
doi = "10.1117/12.2005760",
language = "English",
isbn = "9780819493347",
volume = "8565",
booktitle = "Photonic Therapeutics and Diagnostics IX",

}

Prabhu, V, Fernandes, EM, Rao, BSS & Mahato, KK 2013, Non-invasive, in vivo fluorescence technique as an objective tool for monitoring wound healing following Low Level Laser Therapy. in Photonic Therapeutics and Diagnostics IX. vol. 8565, 85650L, Photonic Therapeutics and Diagnostics XI, San Francisco, United States, 07-02-15. https://doi.org/10.1117/12.2005760

Non-invasive, in vivo fluorescence technique as an objective tool for monitoring wound healing following Low Level Laser Therapy. / Prabhu, Vijendra; Fernandes, Edward Mark; Rao, Bola Sadashiva Satish; Mahato, Krishna Kishore.

Photonic Therapeutics and Diagnostics IX. Vol. 8565 2013. 85650L.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Non-invasive, in vivo fluorescence technique as an objective tool for monitoring wound healing following Low Level Laser Therapy

AU - Prabhu, Vijendra

AU - Fernandes, Edward Mark

AU - Rao, Bola Sadashiva Satish

AU - Mahato, Krishna Kishore

PY - 2013

Y1 - 2013

N2 - Collagen represents major protein component of the extracellular matrix (ECM), thus its monitoring could be used as an ideal tool for assessing wound healing following therapy. Presently, there is a great need to develop quick, objective, non-destructive method to monitor collagen synthesis during progression of wound healing. The applicability of the Laser induced fluorescence technique towards wound healing monitoring by measuring collagen levels at different stages of the healing progression is the main idea behind the current work. Six to eight weeks old Swiss albino mice with ccircular wounds of 15 mm diameter were illuminated with single exposure of 2 J/cm2 from He-Ne laser (632.8 nm; 7 mW power; 4.02 mW/cm2 power density) along with un-illuminated and un-wounded controls. Spectroscopic changes were monitored by recording in vivo fluorescence from each animal (under anesthesia) at different post-wounding days (5th, 10th, 30 thday) by exciting granulation tissue/skin with 325 nm He-Cd laser. The autofluorescence from the tissue/skin was recorded from four different sites and four spectra were recorded from each site. A total of 2160 spectra were recorded from 45 animals. The in vivo fluorescence studies have shown significant increase (P<0.001) in collagen synthesis upon treatment with optimum laser dose of 2 J/cm2 immediately after wounding as compared to un-illuminated control group. It can conclude that in vivo fluorescence measurement is effective in monitoring wound healing and hence could be used over ex vivo method as an objective and non-intrusive method to monitor collagen levels.

AB - Collagen represents major protein component of the extracellular matrix (ECM), thus its monitoring could be used as an ideal tool for assessing wound healing following therapy. Presently, there is a great need to develop quick, objective, non-destructive method to monitor collagen synthesis during progression of wound healing. The applicability of the Laser induced fluorescence technique towards wound healing monitoring by measuring collagen levels at different stages of the healing progression is the main idea behind the current work. Six to eight weeks old Swiss albino mice with ccircular wounds of 15 mm diameter were illuminated with single exposure of 2 J/cm2 from He-Ne laser (632.8 nm; 7 mW power; 4.02 mW/cm2 power density) along with un-illuminated and un-wounded controls. Spectroscopic changes were monitored by recording in vivo fluorescence from each animal (under anesthesia) at different post-wounding days (5th, 10th, 30 thday) by exciting granulation tissue/skin with 325 nm He-Cd laser. The autofluorescence from the tissue/skin was recorded from four different sites and four spectra were recorded from each site. A total of 2160 spectra were recorded from 45 animals. The in vivo fluorescence studies have shown significant increase (P<0.001) in collagen synthesis upon treatment with optimum laser dose of 2 J/cm2 immediately after wounding as compared to un-illuminated control group. It can conclude that in vivo fluorescence measurement is effective in monitoring wound healing and hence could be used over ex vivo method as an objective and non-intrusive method to monitor collagen levels.

UR - http://www.scopus.com/inward/record.url?scp=84878177880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878177880&partnerID=8YFLogxK

U2 - 10.1117/12.2005760

DO - 10.1117/12.2005760

M3 - Conference contribution

SN - 9780819493347

VL - 8565

BT - Photonic Therapeutics and Diagnostics IX

ER -