TY - JOUR
T1 - Non-typhoidal salmonellosis
T2 - Detection of genes responsible for virulence-A hospital based study from Manipal, India
AU - Bangera, Sohan Rodney
AU - Umakanth, Shashikiran
AU - Chowdhury, Goutam
AU - Saha, Rudra Narayan
AU - Ballal, Mamatha
N1 - Funding Information:
The authors thankfully acknowledge Manipal Academy of Higher Education and Directorate of Minorities, Government of Karnataka, India for providing the research scholarship. Okayama University, Japan for the funding support.The authors are grateful to Dr.Luisa Veira Peixe, Associate Professor, Faculty of Pharmacy, University of Porto, Portugal for providing the standard strains.
Funding Information:
The authors are grateful to Dr.Luisa Veira Peixe, Associate Professor, Faculty of Pharmacy, University of Porto, Portugal for providing the standard strains.
Publisher Copyright:
© Journal of Krishna Institute of Medical Sciences University.
PY - 2022/7/1
Y1 - 2022/7/1
N2 - Background: Non-typhoidal Salmonella (NTS) is one of the four critical worldwide reasons for diarrhoeal infections and causes a noted zoonotic infection termed non-typhoidal salmonellosis. Non-typhoidal salmonellosis generally causes self-limited gastroenteritis, whereas in immunocompromised conditions can result in invasive infections. Aim and Objectives: To detect the likely NTS serovars causing non-typhoidal Salmonellosis and their virulence genes. Material and Methods: This is a prospective cross-sectional research work carried out at the Enteric Diseases Division, Central Research Lab, Kasturba Medical College, Manipal from January 2016-June 2018. Stool samples were collected from patients with diarrhoea admitted to the tertiary care centre in Udupi district, India. Stool samples were processed according to the World Health Organisation laboratory protocol. Non-typhoidal Salmonella isolated were subjected to PCR for the detection of virulence genes. Results: Of the 1599 diarrheal samples processed, 55 NTS were isolated with a prevalence of 3.43 %. invA gene was existent in 83.6% of the isolates, spvC gene in 61.8%, stn in 100% sopB in 96.4% and sefA in 5.45%. Conclusion: The presence of virulence genes among NTS increases the complications of non-typhoidal salmonellosis. Routine antimicrobial susceptibility tests for salmonellae should be carried out and then reported to prevent the associated morbidity and mortality.
AB - Background: Non-typhoidal Salmonella (NTS) is one of the four critical worldwide reasons for diarrhoeal infections and causes a noted zoonotic infection termed non-typhoidal salmonellosis. Non-typhoidal salmonellosis generally causes self-limited gastroenteritis, whereas in immunocompromised conditions can result in invasive infections. Aim and Objectives: To detect the likely NTS serovars causing non-typhoidal Salmonellosis and their virulence genes. Material and Methods: This is a prospective cross-sectional research work carried out at the Enteric Diseases Division, Central Research Lab, Kasturba Medical College, Manipal from January 2016-June 2018. Stool samples were collected from patients with diarrhoea admitted to the tertiary care centre in Udupi district, India. Stool samples were processed according to the World Health Organisation laboratory protocol. Non-typhoidal Salmonella isolated were subjected to PCR for the detection of virulence genes. Results: Of the 1599 diarrheal samples processed, 55 NTS were isolated with a prevalence of 3.43 %. invA gene was existent in 83.6% of the isolates, spvC gene in 61.8%, stn in 100% sopB in 96.4% and sefA in 5.45%. Conclusion: The presence of virulence genes among NTS increases the complications of non-typhoidal salmonellosis. Routine antimicrobial susceptibility tests for salmonellae should be carried out and then reported to prevent the associated morbidity and mortality.
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M3 - Article
AN - SCOPUS:85144079089
VL - 11
SP - 1
EP - 11
JO - Journal of Krishna Institute of Medical Sciences University
JF - Journal of Krishna Institute of Medical Sciences University
SN - 2231-4261
IS - 3
ER -
