Novel pH-sensitive interpenetrated network polyspheres of polyacrylamide-g-locust bean gum and sodium alginate for intestinal targeting of ketoprofen: In vitro and in vivo evaluation

Rashmi Boppana, Sushil Yadaorao Raut, G. Krishna Mohan, Biswanath Sa, Srinivas Mutalik, Kakarla Raghava Reddy, Kusal K. Das, Mallanagouda S. Biradar, Raghavendra V. Kulkarni

Research output: Contribution to journalArticle

Abstract

In this report, novel pH-sensitive interpenetrated network (IPN)polyspheres were developed utilizing polyacrylamide-g-locust bean gum (PAAm-g-LBG)in combination with sodium alginate (SA)to achieve intestinal targeted delivery of ketoprofen. PAAm-g-LBG was synthesized under microwave irradiation wherein ceric ammonium nitrate was used as reaction initiator and then conversion of PAAm-g-LBG as pH-sensitive copolymer was carried out by alkaline hydrolysis. The PAAm-g-LBG copolymer was characterized through 1 H-NMR, FTIR and elemental analysis. The IPN polyspheres exhibited pH-depended swelling or de-swelling with the alteration of surrounding pH. The in-vitro release of drug from IPN polyspheres was found to be higher (≈ 90%)in phosphate buffer of pH 7.4 in comparison with that in pH 1.2 buffer (10.6%). The in-vivo pharmacokinetic, anti-inflammatory screening and stomach histopathology studies performed on Wistar rats revealed pH sensitivity of IPN polyspheres where ketoprofen was successfully targeted to small intestine resulting in reduced side effects of ketoprofen like ulcer formation, erosion of gastric mucosa and hemorrhages.

Original languageEnglish
Pages (from-to)362-370
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume180
DOIs
Publication statusPublished - 01-08-2019

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locusts
Ketoprofen
Sodium alginate
Polyacrylates
sodium
evaluation
Swelling
Buffers
Copolymers
Pharmacokinetics
Microwave irradiation
swelling
copolymers
buffers
Rats
Erosion
Nitrates
Hydrolysis
Screening
Phosphates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Boppana, Rashmi ; Yadaorao Raut, Sushil ; Krishna Mohan, G. ; Sa, Biswanath ; Mutalik, Srinivas ; Reddy, Kakarla Raghava ; Das, Kusal K. ; Biradar, Mallanagouda S. ; Kulkarni, Raghavendra V. / Novel pH-sensitive interpenetrated network polyspheres of polyacrylamide-g-locust bean gum and sodium alginate for intestinal targeting of ketoprofen : In vitro and in vivo evaluation. In: Colloids and Surfaces B: Biointerfaces. 2019 ; Vol. 180. pp. 362-370.
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abstract = "In this report, novel pH-sensitive interpenetrated network (IPN)polyspheres were developed utilizing polyacrylamide-g-locust bean gum (PAAm-g-LBG)in combination with sodium alginate (SA)to achieve intestinal targeted delivery of ketoprofen. PAAm-g-LBG was synthesized under microwave irradiation wherein ceric ammonium nitrate was used as reaction initiator and then conversion of PAAm-g-LBG as pH-sensitive copolymer was carried out by alkaline hydrolysis. The PAAm-g-LBG copolymer was characterized through 1 H-NMR, FTIR and elemental analysis. The IPN polyspheres exhibited pH-depended swelling or de-swelling with the alteration of surrounding pH. The in-vitro release of drug from IPN polyspheres was found to be higher (≈ 90{\%})in phosphate buffer of pH 7.4 in comparison with that in pH 1.2 buffer (10.6{\%}). The in-vivo pharmacokinetic, anti-inflammatory screening and stomach histopathology studies performed on Wistar rats revealed pH sensitivity of IPN polyspheres where ketoprofen was successfully targeted to small intestine resulting in reduced side effects of ketoprofen like ulcer formation, erosion of gastric mucosa and hemorrhages.",
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Novel pH-sensitive interpenetrated network polyspheres of polyacrylamide-g-locust bean gum and sodium alginate for intestinal targeting of ketoprofen : In vitro and in vivo evaluation. / Boppana, Rashmi; Yadaorao Raut, Sushil; Krishna Mohan, G.; Sa, Biswanath; Mutalik, Srinivas; Reddy, Kakarla Raghava; Das, Kusal K.; Biradar, Mallanagouda S.; Kulkarni, Raghavendra V.

In: Colloids and Surfaces B: Biointerfaces, Vol. 180, 01.08.2019, p. 362-370.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Novel pH-sensitive interpenetrated network polyspheres of polyacrylamide-g-locust bean gum and sodium alginate for intestinal targeting of ketoprofen

T2 - In vitro and in vivo evaluation

AU - Boppana, Rashmi

AU - Yadaorao Raut, Sushil

AU - Krishna Mohan, G.

AU - Sa, Biswanath

AU - Mutalik, Srinivas

AU - Reddy, Kakarla Raghava

AU - Das, Kusal K.

AU - Biradar, Mallanagouda S.

AU - Kulkarni, Raghavendra V.

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