Oocytes recovered after ovarian tissue slow freezing have impaired H2AX phosphorylation and functional competence

Sam Sudhakaran, Shubhashree Uppangala, Sujith Raj Salian, Sachin D. Honguntikar, Ramya Nair, Guruprasad Kalthur, Satish Kumar Adiga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

It has been shown that oocytes isolated from ovarian tissue cryopreservation acquire DNA damage during the process of freeze-thawing. Using a mouse model, here we have investigated the functional competence and phosphorylation of H2AX (γ-H2AX) in germinal vesicle (GV) and parthenogenetically activated oocytes derived from conventional ovarian tissue slow freezing and vitrification techniques. The number of GV-stage oocytes with γ-H2AX foci was not significantly different between the slow-freezing and vitrification groups. Although the in vitro maturation (IVM) potential of GV oocytes in the slow-freezing group showed a significant delay (P<0.0001) in the process of germinal vesicle breakdown, no difference in the maturation rate was observed between the two protocols. Nevertheless, parthenogenetic activation of IVM oocytes using strontium chloride showed a significantly lower activation rate in the slow-freezing group compared with the vitrification (P<0.05) and control (P<0.01) groups. Importantly, H2AX phosphorylation was significantly perturbed in the slow-freezing group in comparison to the control (P<0.05). Therefore, we conclude that impaired sensing of DNA strand breaks and repair processes are associated with the reduced functional competence of the oocytes recovered from the slow-freezing group, which may have a significant impact on the reproductive outcome.

Original languageEnglish
Pages (from-to)1242-1248
Number of pages7
JournalReproduction, Fertility and Development
Volume27
Issue number8
DOIs
Publication statusPublished - 2015

Fingerprint

Mental Competency
Freezing
Oocytes
phosphorylation
freezing
germinal vesicle
oocytes
Phosphorylation
Vitrification
vitrification
In Vitro Oocyte Maturation Techniques
DNA Breaks
strontium
Cryopreservation
thawing
DNA Repair
DNA damage
cryopreservation
DNA Damage
tissues

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Reproductive Medicine
  • Molecular Biology
  • Animal Science and Zoology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

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title = "Oocytes recovered after ovarian tissue slow freezing have impaired H2AX phosphorylation and functional competence",
abstract = "It has been shown that oocytes isolated from ovarian tissue cryopreservation acquire DNA damage during the process of freeze-thawing. Using a mouse model, here we have investigated the functional competence and phosphorylation of H2AX (γ-H2AX) in germinal vesicle (GV) and parthenogenetically activated oocytes derived from conventional ovarian tissue slow freezing and vitrification techniques. The number of GV-stage oocytes with γ-H2AX foci was not significantly different between the slow-freezing and vitrification groups. Although the in vitro maturation (IVM) potential of GV oocytes in the slow-freezing group showed a significant delay (P<0.0001) in the process of germinal vesicle breakdown, no difference in the maturation rate was observed between the two protocols. Nevertheless, parthenogenetic activation of IVM oocytes using strontium chloride showed a significantly lower activation rate in the slow-freezing group compared with the vitrification (P<0.05) and control (P<0.01) groups. Importantly, H2AX phosphorylation was significantly perturbed in the slow-freezing group in comparison to the control (P<0.05). Therefore, we conclude that impaired sensing of DNA strand breaks and repair processes are associated with the reduced functional competence of the oocytes recovered from the slow-freezing group, which may have a significant impact on the reproductive outcome.",
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Oocytes recovered after ovarian tissue slow freezing have impaired H2AX phosphorylation and functional competence. / Sudhakaran, Sam; Uppangala, Shubhashree; Salian, Sujith Raj; Honguntikar, Sachin D.; Nair, Ramya; Kalthur, Guruprasad; Adiga, Satish Kumar.

In: Reproduction, Fertility and Development, Vol. 27, No. 8, 2015, p. 1242-1248.

Research output: Contribution to journalArticle

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AU - Uppangala, Shubhashree

AU - Salian, Sujith Raj

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AU - Nair, Ramya

AU - Kalthur, Guruprasad

AU - Adiga, Satish Kumar

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