Optical diagnosis of cervical cancer by fluorescence spectroscopy technique

Siddappa M. Chidananda, Kapaettu Satyamoorthy, Lavanya Rai, Attibele P. Manjunath, Vasudevan B. Kartha

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

In the present work, we examine normal and malignant stage IIIB cervical tissue by laser induced fluorescence, with 2 different objectives. (i) Development of the fluorescence spectroscopy technique as a standard optical method for discrimination of normal and malignant tissue samples and, (ii) Optimization of the technique by the method of matching of a sample spectrum with calibration sets of spectra of pathologically certified samples. Laser-induced fluorescence spectra were measured using samples from 62 subjects at different excitation wavelengths. Principal component analysis (PCA) of spectra and intensity ratios of curve-resolved fluorescence peaks were tested for discrimination. It was found that PCA of total fluorescence at 325 nm excitation gives specificity and sensitivity over 95%. Use of calibration sets of spectra of histo-pathologically certified samples combined with PCA for matching and pass/fail classification of test samples is shown to have high sensitivity/specificity for routine diagnostic purposes as well as for possible staging of the disease. Further, the multi-component origin of the fluorescence spectra is illustrated by curve resolution and fluorescence spectra of separated proteins of tissue homogenates.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalInternational Journal of Cancer
Volume119
Issue number1
DOIs
Publication statusPublished - 01-07-2006

Fingerprint

Fluorescence Spectrometry
Uterine Cervical Neoplasms
Fluorescence
Principal Component Analysis
Calibration
Lasers
Sensitivity and Specificity
Proteins

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

Cite this

Chidananda, Siddappa M. ; Satyamoorthy, Kapaettu ; Rai, Lavanya ; Manjunath, Attibele P. ; Kartha, Vasudevan B. / Optical diagnosis of cervical cancer by fluorescence spectroscopy technique. In: International Journal of Cancer. 2006 ; Vol. 119, No. 1. pp. 139-145.
@article{e65d53e084394164bba4cb6c42c0c0f3,
title = "Optical diagnosis of cervical cancer by fluorescence spectroscopy technique",
abstract = "In the present work, we examine normal and malignant stage IIIB cervical tissue by laser induced fluorescence, with 2 different objectives. (i) Development of the fluorescence spectroscopy technique as a standard optical method for discrimination of normal and malignant tissue samples and, (ii) Optimization of the technique by the method of matching of a sample spectrum with calibration sets of spectra of pathologically certified samples. Laser-induced fluorescence spectra were measured using samples from 62 subjects at different excitation wavelengths. Principal component analysis (PCA) of spectra and intensity ratios of curve-resolved fluorescence peaks were tested for discrimination. It was found that PCA of total fluorescence at 325 nm excitation gives specificity and sensitivity over 95{\%}. Use of calibration sets of spectra of histo-pathologically certified samples combined with PCA for matching and pass/fail classification of test samples is shown to have high sensitivity/specificity for routine diagnostic purposes as well as for possible staging of the disease. Further, the multi-component origin of the fluorescence spectra is illustrated by curve resolution and fluorescence spectra of separated proteins of tissue homogenates.",
author = "Chidananda, {Siddappa M.} and Kapaettu Satyamoorthy and Lavanya Rai and Manjunath, {Attibele P.} and Kartha, {Vasudevan B.}",
year = "2006",
month = "7",
day = "1",
doi = "10.1002/ijc.21825",
language = "English",
volume = "119",
pages = "139--145",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "1",

}

Optical diagnosis of cervical cancer by fluorescence spectroscopy technique. / Chidananda, Siddappa M.; Satyamoorthy, Kapaettu; Rai, Lavanya; Manjunath, Attibele P.; Kartha, Vasudevan B.

In: International Journal of Cancer, Vol. 119, No. 1, 01.07.2006, p. 139-145.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Optical diagnosis of cervical cancer by fluorescence spectroscopy technique

AU - Chidananda, Siddappa M.

AU - Satyamoorthy, Kapaettu

AU - Rai, Lavanya

AU - Manjunath, Attibele P.

AU - Kartha, Vasudevan B.

PY - 2006/7/1

Y1 - 2006/7/1

N2 - In the present work, we examine normal and malignant stage IIIB cervical tissue by laser induced fluorescence, with 2 different objectives. (i) Development of the fluorescence spectroscopy technique as a standard optical method for discrimination of normal and malignant tissue samples and, (ii) Optimization of the technique by the method of matching of a sample spectrum with calibration sets of spectra of pathologically certified samples. Laser-induced fluorescence spectra were measured using samples from 62 subjects at different excitation wavelengths. Principal component analysis (PCA) of spectra and intensity ratios of curve-resolved fluorescence peaks were tested for discrimination. It was found that PCA of total fluorescence at 325 nm excitation gives specificity and sensitivity over 95%. Use of calibration sets of spectra of histo-pathologically certified samples combined with PCA for matching and pass/fail classification of test samples is shown to have high sensitivity/specificity for routine diagnostic purposes as well as for possible staging of the disease. Further, the multi-component origin of the fluorescence spectra is illustrated by curve resolution and fluorescence spectra of separated proteins of tissue homogenates.

AB - In the present work, we examine normal and malignant stage IIIB cervical tissue by laser induced fluorescence, with 2 different objectives. (i) Development of the fluorescence spectroscopy technique as a standard optical method for discrimination of normal and malignant tissue samples and, (ii) Optimization of the technique by the method of matching of a sample spectrum with calibration sets of spectra of pathologically certified samples. Laser-induced fluorescence spectra were measured using samples from 62 subjects at different excitation wavelengths. Principal component analysis (PCA) of spectra and intensity ratios of curve-resolved fluorescence peaks were tested for discrimination. It was found that PCA of total fluorescence at 325 nm excitation gives specificity and sensitivity over 95%. Use of calibration sets of spectra of histo-pathologically certified samples combined with PCA for matching and pass/fail classification of test samples is shown to have high sensitivity/specificity for routine diagnostic purposes as well as for possible staging of the disease. Further, the multi-component origin of the fluorescence spectra is illustrated by curve resolution and fluorescence spectra of separated proteins of tissue homogenates.

UR - http://www.scopus.com/inward/record.url?scp=33646508776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646508776&partnerID=8YFLogxK

U2 - 10.1002/ijc.21825

DO - 10.1002/ijc.21825

M3 - Article

VL - 119

SP - 139

EP - 145

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 1

ER -