Optical diagnosis of cervical cancer by fluorescence spectroscopy technique

Siddappa M. Chidananda, Kapaettu Satyamoorthy, Lavanya Rai, Attibele P. Manjunath, Vasudevan B. Kartha

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

In the present work, we examine normal and malignant stage IIIB cervical tissue by laser induced fluorescence, with 2 different objectives. (i) Development of the fluorescence spectroscopy technique as a standard optical method for discrimination of normal and malignant tissue samples and, (ii) Optimization of the technique by the method of matching of a sample spectrum with calibration sets of spectra of pathologically certified samples. Laser-induced fluorescence spectra were measured using samples from 62 subjects at different excitation wavelengths. Principal component analysis (PCA) of spectra and intensity ratios of curve-resolved fluorescence peaks were tested for discrimination. It was found that PCA of total fluorescence at 325 nm excitation gives specificity and sensitivity over 95%. Use of calibration sets of spectra of histo-pathologically certified samples combined with PCA for matching and pass/fail classification of test samples is shown to have high sensitivity/specificity for routine diagnostic purposes as well as for possible staging of the disease. Further, the multi-component origin of the fluorescence spectra is illustrated by curve resolution and fluorescence spectra of separated proteins of tissue homogenates.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalInternational Journal of Cancer
Volume119
Issue number1
DOIs
Publication statusPublished - 01-07-2006

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

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