Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology

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Aqueous two-phase system (ATPS) composed of polyethylene glycol 6000 (PEG 6000) and sodium citrate (SC) has been proposed to recover the valuable soluble proteins from tannery wastewater. A sequential optimization strategy which included fractional factorial design (fFD) and central composite design (CCD) was employed to enhance the recovery. From this strategy, a second-order polynomial model was obtained for the protein recovery and it was validated. The optimum recovery was found as 93.46% when pH, NaCl concentration, and temperature were kept at 7.5, 0.1 M, and 33°C, respectively, for a phase system composed of 20% (w/w) PEG 6000-15% (w/w) SC. Thus the proposed ATPS can serve as an alternative to the conventional precipitation method to recover the soluble proteins from tannery wastewater.

Original languageEnglish
Article number217483
JournalJournal of Engineering (United States)
Publication statusPublished - 01-01-2013


All Science Journal Classification (ASJC) codes

  • Civil and Structural Engineering
  • Industrial and Manufacturing Engineering
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Chemical Engineering(all)
  • Hardware and Architecture

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