Optimization of inulinase production by a newly isolated strain Aspergillus flavus var. flavus by solid state fermentation of Saccharum arundinaceum

Deblina Das, Raja Selvaraj, M. Ramananda Bhat

Research output: Contribution to journalArticle

Abstract

The present study deals with the use of mangrove soil, a new source for the isolation of an inulinase producing strain followed by the enzyme production and optimization using hardy sugarcane, “Saccharum arundinaceum”, a novel substrate. Four fungal isolates were obtained from the primary screening in inulin media. In the secondary screening, only Aspergillus flavus var. flavus strain ATCC 16883, a new strain, showed the zone of hydrolysis surrounding the fungal growth on the inulin medium plate. The action pattern of inulinase was determined by thin layer chromatography which confirmed it to be exoinulinase in nature. Solid state fermentation of A. flavus ATCC 16883 using Saccharum arundinaceum showed a maximum inulinase activity of 3.48U/gds after 96 h. The inulinase nature of the enzyme was confirmed by the resultant I/S ratio of 2.56. Central Composite Design (CCD) was used to optimize the media components and obtain a quadratic model using the five significant nutrients namely, inulin, K2HPO4, (NH4)2SO4, KH2PO4 and NaCl. Using the optimum conditions of (g/gds) inulin- 0.1, K2HPO4- 0.05, (NH4)2SO4- 0.002, KH2PO4- 0.005 and NaCl- 0.02, the inulinase activity was determined as 8.57U/gds which was 2.50-fold higher than the initial unoptimized conditions.

Original languageEnglish
Article number101363
JournalBiocatalysis and Agricultural Biotechnology
Volume22
DOIs
Publication statusPublished - 01-11-2019

Fingerprint

inulinase
Aspergillus flavus
Saccharum
solid state fermentation
Aspergillus
Inulin
Fermentation
inulin
Screening
Enzymes
Thin layer chromatography
mangrove soils
screening
Nutrients
Hydrolysis
Thin Layer Chromatography
enzymes
thin layer chromatography
sugarcane
microbial growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

Cite this

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title = "Optimization of inulinase production by a newly isolated strain Aspergillus flavus var. flavus by solid state fermentation of Saccharum arundinaceum",
abstract = "The present study deals with the use of mangrove soil, a new source for the isolation of an inulinase producing strain followed by the enzyme production and optimization using hardy sugarcane, “Saccharum arundinaceum”, a novel substrate. Four fungal isolates were obtained from the primary screening in inulin media. In the secondary screening, only Aspergillus flavus var. flavus strain ATCC 16883, a new strain, showed the zone of hydrolysis surrounding the fungal growth on the inulin medium plate. The action pattern of inulinase was determined by thin layer chromatography which confirmed it to be exoinulinase in nature. Solid state fermentation of A. flavus ATCC 16883 using Saccharum arundinaceum showed a maximum inulinase activity of 3.48U/gds after 96 h. The inulinase nature of the enzyme was confirmed by the resultant I/S ratio of 2.56. Central Composite Design (CCD) was used to optimize the media components and obtain a quadratic model using the five significant nutrients namely, inulin, K2HPO4, (NH4)2SO4, KH2PO4 and NaCl. Using the optimum conditions of (g/gds) inulin- 0.1, K2HPO4- 0.05, (NH4)2SO4- 0.002, KH2PO4- 0.005 and NaCl- 0.02, the inulinase activity was determined as 8.57U/gds which was 2.50-fold higher than the initial unoptimized conditions.",
author = "Deblina Das and Raja Selvaraj and {Ramananda Bhat}, M.",
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