Optimization of reducing sugars production from agro-residue coconut leaflets using autoclave-assisted HCl hydrolysis with response surface methodology

Vinayaka Babu Shet, Chetan Varun, Uday Aishwarya, Anusha Madu Palan, Shama Umesh Rao, Louella Concepta Goveas, Selvaraj Raja, Chandrayan Vaman Rao, Ujwal Puttur

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The utilization of coconut leaflets, an agro residue generated from coconut farming, was investigated to develop a process to release reducing sugars using hydrochloric acid (HCl)-assisted, autoclave-based hydrolysis. The variables affecting HCl hydrolysis (duration of autoclaving, concentration of HCl and weight of coconut leaflets) were screened using a one factor at-a-time approach. The duration of autoclaving and concentration of HCl both had significant effects on the hydrolysis process and the levels of these factors were further optimized using a central composite design with response surface methodology. The optimized conditions were 3.17% of HCl and 44.14 min of autoclaving that released the maximum reducing sugars of 29 g/L. A second order model was generated, which was a good fit with a coefficient of determination value of 0.83.

Original languageEnglish
Pages (from-to)280-284
Number of pages5
JournalAgriculture and Natural Resources
Issue number3
Publication statusPublished - 01-06-2018


All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

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