Oral antidiabetic activity of Annona squamosa leaf alcohol extract in NIDDM rats

A. Shirwaikar, K. Rajendran, C.D. Kumar

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The leaf alcohol extract of the plant Annona squamosa was investigated for its antidiabetic activity. Type 2 diabetes mellitus was induced with standardised doses of streptozotocin and nicotinamide. Graded doses of the leaf alcohol extract suspended in gum acacia were administered to normal and experimental diabetic rats for 12 days. Fasting plasma glucose levels, serum insulin levels, serum lipid profiles and changes in body weight were evaluated in normal rats while liver glycogen levels and pancreatic TBARS levels were evaluated additionally in diabetic rats. The diabetic groups treated with the leaf alcohol extract were compared with standard glibenclamide. The findings showed the significant antidiabetic potential of the extract in ameliorating the diabetic conditions in diabetic rats. No significant effects were found in the normal rats.
Original languageEnglish
Pages (from-to)30-35
Number of pages6
JournalPharmaceutical Biology
Volume42
Issue number1
DOIs
Publication statusPublished - 2004

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Annona
Hypoglycemic Agents
Type 2 Diabetes Mellitus
Alcohols
Gum Arabic
Liver Glycogen
Body Weight Changes
Niacinamide
Glyburide
Plant Extracts
Streptozocin
Serum
Fasting
Insulin
Lipids
Glucose

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Shirwaikar, A. ; Rajendran, K. ; Kumar, C.D. / Oral antidiabetic activity of Annona squamosa leaf alcohol extract in NIDDM rats. In: Pharmaceutical Biology. 2004 ; Vol. 42, No. 1. pp. 30-35.
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title = "Oral antidiabetic activity of Annona squamosa leaf alcohol extract in NIDDM rats",
abstract = "The leaf alcohol extract of the plant Annona squamosa was investigated for its antidiabetic activity. Type 2 diabetes mellitus was induced with standardised doses of streptozotocin and nicotinamide. Graded doses of the leaf alcohol extract suspended in gum acacia were administered to normal and experimental diabetic rats for 12 days. Fasting plasma glucose levels, serum insulin levels, serum lipid profiles and changes in body weight were evaluated in normal rats while liver glycogen levels and pancreatic TBARS levels were evaluated additionally in diabetic rats. The diabetic groups treated with the leaf alcohol extract were compared with standard glibenclamide. The findings showed the significant antidiabetic potential of the extract in ameliorating the diabetic conditions in diabetic rats. No significant effects were found in the normal rats.",
author = "A. Shirwaikar and K. Rajendran and C.D. Kumar",
note = "Cited By :32 Export Date: 10 November 2017 CODEN: PHBIF Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, College of Pharmaceutical Sciences, Manipal-576 119, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: alcohol, 64-17-5; glibenclamide, 10238-21-8; glucose, 50-99-7, 84778-64-3; glycogen, 9005-79-2; gum arabic, 9000-01-5; insulin, 9004-10-8; lipid, 66455-18-3; nicotinamide, 11032-50-1, 98-92-0 References: Asolkar, L.V., Kakkar, K.K., Chakre, O.J., (1992) Glossary of Indian Medicinal Plants with Active Principles, pp. 72-73. , Publications and Information Directorate, New Delhi; Atique, A., Iqbal, M., Ghouse, A.K.M., Use of Annona squamosa and Piper nigrum against diabetes (1985) Fitoterapia, 56, pp. 190-192; Bailey, C.J., Day, C., Traditional treatments for diabetes (1989) Diabetes Care, 12, pp. 553-564; Baynes, J.W., Role of oxidative stress in development of complications in diabetes (1991) Diabetes, 40, pp. 405-412; Bonner-Weir, S., Morphological evidence of pancreatic polarity of beta cells within islets of langerhans (1988) Diabetes, 37, pp. 616-621; Chase, P.H., Glasgow, A.M., Juvenile diabetes mellitus and serum lipids and lipoprotein levels (1976) Am J Dis Child, 130, pp. 1113-1117; Erah, P.O., Osuide, G.E., Omogbai, E.K.I., Hypoglycemic effect of the extract of Solenostemon monostachys leaves (1996) J West Afr Pharm, 10, pp. 21-27; Ford, R.U., Diabetes mellitus and serum carotenoids findings from the third National and Nutrition Examination Survey (1999) Amer J Epidem, 149, pp. 168-176; Ghosh, M.N., Toxicity studies (1984) Fundamentals of Experimental Pharmacology, pp. 153-158. , Scientific Book Agency, Calcutta; Halliwell, B., Gutteridge, J.M.C., (1985) Free Radicals in Biology and Medicine, pp. 24-86. , Oxford Clarendon Press, London; Harborne, J.B., (1998) Phytochemical Methods, pp. 60-66. , Chapman and Hall, London; Hideaki, K., Kajimoto, Y., Miyagawa, J., Matsuoka, T., Fujitani, Y., Umayahara, Y., Hanafusa, T., Yamasaki, Y., Beneficial effects of antioxidants in diabetes (1999) Diabetes, 48, pp. 2398-2401; Hiroshi, O., Nobuko, O., Kunio, V., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 5, pp. 351-358; Huang, X., Vaag, A., Hanson, M., Weng, J., Goop, L., Impaired insulin stimulated expression of the glycogen synthase gene in skeletal muscle of type 2 diabetic patients is aquired rather than inherited (2000) J Clin Endocrinol Metab, 85, pp. 1584-1590; Baynes, J.W., Thorpe, S.R., Perspectives in diabetes, Role of oxidative stress in diabetic complications. A new perspective on an old paradigm (1999) Diabetes, 48, pp. 1-9; Kokate, C.K., (1994) Practical Pharmacognosy, pp. 107-113. , Vallabh Prakashan, New Delhi; Lamela, M., Cadavid, I., Gato, A., Calleja, J.M., Effect of Lythrum saricaria in normoglycemic rats (1985) J Ethnopharmacol, 41, pp. 83-91; Latner, A., Carbohydrate metabolism, abnormalities of post absorbtive blood sugar level (1958) Clinical Biochemistry, p. 48. , WB. Saunders and Co., Philadelphia; Maxwell, S.R.J., Thomason, H., Sander, D., LeGuen, C., Baxtex, A.M., Thrope, G.H.G., Jones, A.F., Bannet, A.M., Poor glycaemic control is associated with reduced free radical scavenging (antioxidant) activity in non-insulin dependent diabetes mellitus (1997) Annal Clin Biochem, 34, pp. 638-644; Miura, T., Ichiki, H., Hashimoto, I., Iwamoto, N., Kato, M., Kubo, M., Ishihara, E., Tanigawa, K., Antidiabetic activity of a xanthone compound, mangiferin (2001) Phytomedicine, 8, pp. 85-87; Caroll, N.V., Longley, R.W., Roe, J.H., The determination of glycogen in liver and muscle by use of anthrone reagent (1956) J Biol Chem, 220, pp. 583-593; Nikkila, E.A., Kekki, M., Plasma tiglyceride transport kinetics in diabetes mellitus (1973) Metabolism, 8, pp. 1-22; Olmedilla, M.N., Reference values for retinol, tocopherol and main carotenoids in serum of control and insulin dependent diabetic Spanish subjects (1999) Clin Chem, 43, pp. 1066-1071; Palmer, A.M., Thomas, C.R., Gopaul, N., Dhir, S., Anggard, E.E., Poston, L., Tribe, R.M., Dietary antioxidant supplementation reduces lipid peroxidation but impairs vascular function in small mesentric arteries of the streptozotocin diabetic rats (1998) Diabetologia, 41, pp. 148-156; Pari, L., Latha, M., Antidiabetic activity of Cassia auriculata flowers: Effect on lipid peroxidation in streptozotocin diabetes rats (2002) Pharm Biol, 40, pp. 512-517; Pellegrino Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire-Buys, D., Novelli, M., Ribes, G., Development of a new model of type II diabetes in adult rats administered with streptozotocin and nicotinamide (1998) Diabetes, 47, pp. 224-229; Rahman, A.U., Zaman, K., Medicinal plants with hypoglycaemic activity (1989) J Ethnopharmacol, 26, pp. 1-55; Rao, V.S.N., Dasaradhan, P., Krishnaiah, K.S., Antifertility effect of some indigenous plants (1979) Ind J Med Res, 70, pp. 517-520; Swanston-Flatt, S.K., Day, C., Bailey, C.J., Flatt, P.R., Traditional plant treatments for diabetes: Studies in normal and streptozotocin diabetic mice (1990) Diabetologia, 33, pp. 462-464; Topno, K.K., Plants used by tribals of Chotanagpur against diabetes (1997) Botanica, 47, pp. 99-101; Turner, M.A., (1965) Screening Methods in Pharmacology, p. 26. , Academic Press, New York; Whitton, P.D., Hems, D.A., Glycogen synthesis in perfused liver of streptozotocin diabetic rats (1975) Biochem J, 150, pp. 153-159; Ye, F., Shen, Z., Xie, M., Alpha glucosidase inhibition from a Chinese medicinal herb (Romulus mori) in normal and diabetic rats and mice (2002) Phytomedicine, 9, pp. 161-166",
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volume = "42",
pages = "30--35",
journal = "Pharmaceutical Biology",
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}

Oral antidiabetic activity of Annona squamosa leaf alcohol extract in NIDDM rats. / Shirwaikar, A.; Rajendran, K.; Kumar, C.D.

In: Pharmaceutical Biology, Vol. 42, No. 1, 2004, p. 30-35.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oral antidiabetic activity of Annona squamosa leaf alcohol extract in NIDDM rats

AU - Shirwaikar, A.

AU - Rajendran, K.

AU - Kumar, C.D.

N1 - Cited By :32 Export Date: 10 November 2017 CODEN: PHBIF Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, College of Pharmaceutical Sciences, Manipal-576 119, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: alcohol, 64-17-5; glibenclamide, 10238-21-8; glucose, 50-99-7, 84778-64-3; glycogen, 9005-79-2; gum arabic, 9000-01-5; insulin, 9004-10-8; lipid, 66455-18-3; nicotinamide, 11032-50-1, 98-92-0 References: Asolkar, L.V., Kakkar, K.K., Chakre, O.J., (1992) Glossary of Indian Medicinal Plants with Active Principles, pp. 72-73. , Publications and Information Directorate, New Delhi; Atique, A., Iqbal, M., Ghouse, A.K.M., Use of Annona squamosa and Piper nigrum against diabetes (1985) Fitoterapia, 56, pp. 190-192; Bailey, C.J., Day, C., Traditional treatments for diabetes (1989) Diabetes Care, 12, pp. 553-564; Baynes, J.W., Role of oxidative stress in development of complications in diabetes (1991) Diabetes, 40, pp. 405-412; Bonner-Weir, S., Morphological evidence of pancreatic polarity of beta cells within islets of langerhans (1988) Diabetes, 37, pp. 616-621; Chase, P.H., Glasgow, A.M., Juvenile diabetes mellitus and serum lipids and lipoprotein levels (1976) Am J Dis Child, 130, pp. 1113-1117; Erah, P.O., Osuide, G.E., Omogbai, E.K.I., Hypoglycemic effect of the extract of Solenostemon monostachys leaves (1996) J West Afr Pharm, 10, pp. 21-27; Ford, R.U., Diabetes mellitus and serum carotenoids findings from the third National and Nutrition Examination Survey (1999) Amer J Epidem, 149, pp. 168-176; Ghosh, M.N., Toxicity studies (1984) Fundamentals of Experimental Pharmacology, pp. 153-158. , Scientific Book Agency, Calcutta; Halliwell, B., Gutteridge, J.M.C., (1985) Free Radicals in Biology and Medicine, pp. 24-86. , Oxford Clarendon Press, London; Harborne, J.B., (1998) Phytochemical Methods, pp. 60-66. , Chapman and Hall, London; Hideaki, K., Kajimoto, Y., Miyagawa, J., Matsuoka, T., Fujitani, Y., Umayahara, Y., Hanafusa, T., Yamasaki, Y., Beneficial effects of antioxidants in diabetes (1999) Diabetes, 48, pp. 2398-2401; Hiroshi, O., Nobuko, O., Kunio, V., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 5, pp. 351-358; Huang, X., Vaag, A., Hanson, M., Weng, J., Goop, L., Impaired insulin stimulated expression of the glycogen synthase gene in skeletal muscle of type 2 diabetic patients is aquired rather than inherited (2000) J Clin Endocrinol Metab, 85, pp. 1584-1590; Baynes, J.W., Thorpe, S.R., Perspectives in diabetes, Role of oxidative stress in diabetic complications. A new perspective on an old paradigm (1999) Diabetes, 48, pp. 1-9; Kokate, C.K., (1994) Practical Pharmacognosy, pp. 107-113. , Vallabh Prakashan, New Delhi; Lamela, M., Cadavid, I., Gato, A., Calleja, J.M., Effect of Lythrum saricaria in normoglycemic rats (1985) J Ethnopharmacol, 41, pp. 83-91; Latner, A., Carbohydrate metabolism, abnormalities of post absorbtive blood sugar level (1958) Clinical Biochemistry, p. 48. , WB. Saunders and Co., Philadelphia; Maxwell, S.R.J., Thomason, H., Sander, D., LeGuen, C., Baxtex, A.M., Thrope, G.H.G., Jones, A.F., Bannet, A.M., Poor glycaemic control is associated with reduced free radical scavenging (antioxidant) activity in non-insulin dependent diabetes mellitus (1997) Annal Clin Biochem, 34, pp. 638-644; Miura, T., Ichiki, H., Hashimoto, I., Iwamoto, N., Kato, M., Kubo, M., Ishihara, E., Tanigawa, K., Antidiabetic activity of a xanthone compound, mangiferin (2001) Phytomedicine, 8, pp. 85-87; Caroll, N.V., Longley, R.W., Roe, J.H., The determination of glycogen in liver and muscle by use of anthrone reagent (1956) J Biol Chem, 220, pp. 583-593; Nikkila, E.A., Kekki, M., Plasma tiglyceride transport kinetics in diabetes mellitus (1973) Metabolism, 8, pp. 1-22; Olmedilla, M.N., Reference values for retinol, tocopherol and main carotenoids in serum of control and insulin dependent diabetic Spanish subjects (1999) Clin Chem, 43, pp. 1066-1071; Palmer, A.M., Thomas, C.R., Gopaul, N., Dhir, S., Anggard, E.E., Poston, L., Tribe, R.M., Dietary antioxidant supplementation reduces lipid peroxidation but impairs vascular function in small mesentric arteries of the streptozotocin diabetic rats (1998) Diabetologia, 41, pp. 148-156; Pari, L., Latha, M., Antidiabetic activity of Cassia auriculata flowers: Effect on lipid peroxidation in streptozotocin diabetes rats (2002) Pharm Biol, 40, pp. 512-517; Pellegrino Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire-Buys, D., Novelli, M., Ribes, G., Development of a new model of type II diabetes in adult rats administered with streptozotocin and nicotinamide (1998) Diabetes, 47, pp. 224-229; Rahman, A.U., Zaman, K., Medicinal plants with hypoglycaemic activity (1989) J Ethnopharmacol, 26, pp. 1-55; Rao, V.S.N., Dasaradhan, P., Krishnaiah, K.S., Antifertility effect of some indigenous plants (1979) Ind J Med Res, 70, pp. 517-520; Swanston-Flatt, S.K., Day, C., Bailey, C.J., Flatt, P.R., Traditional plant treatments for diabetes: Studies in normal and streptozotocin diabetic mice (1990) Diabetologia, 33, pp. 462-464; Topno, K.K., Plants used by tribals of Chotanagpur against diabetes (1997) Botanica, 47, pp. 99-101; Turner, M.A., (1965) Screening Methods in Pharmacology, p. 26. , Academic Press, New York; Whitton, P.D., Hems, D.A., Glycogen synthesis in perfused liver of streptozotocin diabetic rats (1975) Biochem J, 150, pp. 153-159; Ye, F., Shen, Z., Xie, M., Alpha glucosidase inhibition from a Chinese medicinal herb (Romulus mori) in normal and diabetic rats and mice (2002) Phytomedicine, 9, pp. 161-166

PY - 2004

Y1 - 2004

N2 - The leaf alcohol extract of the plant Annona squamosa was investigated for its antidiabetic activity. Type 2 diabetes mellitus was induced with standardised doses of streptozotocin and nicotinamide. Graded doses of the leaf alcohol extract suspended in gum acacia were administered to normal and experimental diabetic rats for 12 days. Fasting plasma glucose levels, serum insulin levels, serum lipid profiles and changes in body weight were evaluated in normal rats while liver glycogen levels and pancreatic TBARS levels were evaluated additionally in diabetic rats. The diabetic groups treated with the leaf alcohol extract were compared with standard glibenclamide. The findings showed the significant antidiabetic potential of the extract in ameliorating the diabetic conditions in diabetic rats. No significant effects were found in the normal rats.

AB - The leaf alcohol extract of the plant Annona squamosa was investigated for its antidiabetic activity. Type 2 diabetes mellitus was induced with standardised doses of streptozotocin and nicotinamide. Graded doses of the leaf alcohol extract suspended in gum acacia were administered to normal and experimental diabetic rats for 12 days. Fasting plasma glucose levels, serum insulin levels, serum lipid profiles and changes in body weight were evaluated in normal rats while liver glycogen levels and pancreatic TBARS levels were evaluated additionally in diabetic rats. The diabetic groups treated with the leaf alcohol extract were compared with standard glibenclamide. The findings showed the significant antidiabetic potential of the extract in ameliorating the diabetic conditions in diabetic rats. No significant effects were found in the normal rats.

U2 - 10.1080/13880200490505438

DO - 10.1080/13880200490505438

M3 - Article

VL - 42

SP - 30

EP - 35

JO - Pharmaceutical Biology

JF - Pharmaceutical Biology

SN - 1388-0209

IS - 1

ER -