Abstract

Objectives To determine the oral candidal carriage (OCC), activity of virulent factors and fluconazole susceptibility in subjects with type 2 diabetes mellitus (T2DM) and investigate their association with HbA1c measurements. Materials and methods A cross sectional study was conducted on 100 diabetics and 100 healthy volunteers. The virulence was assessed by measuring the phospholipase activity and proteolysis index. Fluconazole susceptibility was performed using the gradient diffusion method. The OCC, virulence factors and antifungal susceptibility were correlated with patients’ HbA1c measurements. Results The OCC and candidal density carriage was significantly higher in diabetics. Candida albicans (C. albicans) was the most frequently isolated species followed by Candida tropicalis (C. tropicalis). Relatively uncommon species, Candida lusitaniae (C. lusitaniae) and Candida lipolytica (C. lipolytica) were isolated from the diabetics. Prevalence of virulence factor, proteinase, was greater in diabetic group (p < 0.05). Reduced fluconazole susceptibility was noted among the isolates from diabetics; however it was not statistically significant (p = 0.593). Except one, all the susceptible-dose dependent and resistant isolates were Candida no-albicans (C. non-albicans). Conclusion C. albicans remains the predominant pathogen in diabetics, although other species are on the rise. Compared to control group, the isolated species from T2DM group had higher proteinase activity. Resistance to fluconazole was considerably greater among the C. non-albicans isolates from T2DM group. These findings warrant effective treatment modalities to reduce the occurrence of oropharyngeal candidiasis.

Original languageEnglish
Pages (from-to)10-19
Number of pages10
JournalDiabetes Research and Clinical Practice
Volume125
DOIs
Publication statusPublished - 01-03-2017

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Fluconazole
Type 2 Diabetes Mellitus
Virulence
Candida
Virulence Factors
Candida albicans
Peptide Hydrolases
Candida tropicalis
Phospholipases
Candidiasis
Proteolysis
Healthy Volunteers
Cross-Sectional Studies
Control Groups
Therapeutics

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

@article{10a1079db1254debbd25b602f0832358,
title = "Oral candidal speciation, virulence and antifungal susceptibility in type 2 diabetes mellitus",
abstract = "Objectives To determine the oral candidal carriage (OCC), activity of virulent factors and fluconazole susceptibility in subjects with type 2 diabetes mellitus (T2DM) and investigate their association with HbA1c measurements. Materials and methods A cross sectional study was conducted on 100 diabetics and 100 healthy volunteers. The virulence was assessed by measuring the phospholipase activity and proteolysis index. Fluconazole susceptibility was performed using the gradient diffusion method. The OCC, virulence factors and antifungal susceptibility were correlated with patients’ HbA1c measurements. Results The OCC and candidal density carriage was significantly higher in diabetics. Candida albicans (C. albicans) was the most frequently isolated species followed by Candida tropicalis (C. tropicalis). Relatively uncommon species, Candida lusitaniae (C. lusitaniae) and Candida lipolytica (C. lipolytica) were isolated from the diabetics. Prevalence of virulence factor, proteinase, was greater in diabetic group (p < 0.05). Reduced fluconazole susceptibility was noted among the isolates from diabetics; however it was not statistically significant (p = 0.593). Except one, all the susceptible-dose dependent and resistant isolates were Candida no-albicans (C. non-albicans). Conclusion C. albicans remains the predominant pathogen in diabetics, although other species are on the rise. Compared to control group, the isolated species from T2DM group had higher proteinase activity. Resistance to fluconazole was considerably greater among the C. non-albicans isolates from T2DM group. These findings warrant effective treatment modalities to reduce the occurrence of oropharyngeal candidiasis.",
author = "Patel, {Pratik N.} and Parul Sah and Chetana Chandrashekar and Sudha Vidyasagar and {Venkata Rao}, J. and Mradul Tiwari and Raghu Radhakrishnan",
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month = "3",
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doi = "10.1016/j.diabres.2017.01.001",
language = "English",
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Oral candidal speciation, virulence and antifungal susceptibility in type 2 diabetes mellitus. / Patel, Pratik N.; Sah, Parul; Chandrashekar, Chetana; Vidyasagar, Sudha; Venkata Rao, J.; Tiwari, Mradul; Radhakrishnan, Raghu.

In: Diabetes Research and Clinical Practice, Vol. 125, 01.03.2017, p. 10-19.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oral candidal speciation, virulence and antifungal susceptibility in type 2 diabetes mellitus

AU - Patel, Pratik N.

AU - Sah, Parul

AU - Chandrashekar, Chetana

AU - Vidyasagar, Sudha

AU - Venkata Rao, J.

AU - Tiwari, Mradul

AU - Radhakrishnan, Raghu

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Objectives To determine the oral candidal carriage (OCC), activity of virulent factors and fluconazole susceptibility in subjects with type 2 diabetes mellitus (T2DM) and investigate their association with HbA1c measurements. Materials and methods A cross sectional study was conducted on 100 diabetics and 100 healthy volunteers. The virulence was assessed by measuring the phospholipase activity and proteolysis index. Fluconazole susceptibility was performed using the gradient diffusion method. The OCC, virulence factors and antifungal susceptibility were correlated with patients’ HbA1c measurements. Results The OCC and candidal density carriage was significantly higher in diabetics. Candida albicans (C. albicans) was the most frequently isolated species followed by Candida tropicalis (C. tropicalis). Relatively uncommon species, Candida lusitaniae (C. lusitaniae) and Candida lipolytica (C. lipolytica) were isolated from the diabetics. Prevalence of virulence factor, proteinase, was greater in diabetic group (p < 0.05). Reduced fluconazole susceptibility was noted among the isolates from diabetics; however it was not statistically significant (p = 0.593). Except one, all the susceptible-dose dependent and resistant isolates were Candida no-albicans (C. non-albicans). Conclusion C. albicans remains the predominant pathogen in diabetics, although other species are on the rise. Compared to control group, the isolated species from T2DM group had higher proteinase activity. Resistance to fluconazole was considerably greater among the C. non-albicans isolates from T2DM group. These findings warrant effective treatment modalities to reduce the occurrence of oropharyngeal candidiasis.

AB - Objectives To determine the oral candidal carriage (OCC), activity of virulent factors and fluconazole susceptibility in subjects with type 2 diabetes mellitus (T2DM) and investigate their association with HbA1c measurements. Materials and methods A cross sectional study was conducted on 100 diabetics and 100 healthy volunteers. The virulence was assessed by measuring the phospholipase activity and proteolysis index. Fluconazole susceptibility was performed using the gradient diffusion method. The OCC, virulence factors and antifungal susceptibility were correlated with patients’ HbA1c measurements. Results The OCC and candidal density carriage was significantly higher in diabetics. Candida albicans (C. albicans) was the most frequently isolated species followed by Candida tropicalis (C. tropicalis). Relatively uncommon species, Candida lusitaniae (C. lusitaniae) and Candida lipolytica (C. lipolytica) were isolated from the diabetics. Prevalence of virulence factor, proteinase, was greater in diabetic group (p < 0.05). Reduced fluconazole susceptibility was noted among the isolates from diabetics; however it was not statistically significant (p = 0.593). Except one, all the susceptible-dose dependent and resistant isolates were Candida no-albicans (C. non-albicans). Conclusion C. albicans remains the predominant pathogen in diabetics, although other species are on the rise. Compared to control group, the isolated species from T2DM group had higher proteinase activity. Resistance to fluconazole was considerably greater among the C. non-albicans isolates from T2DM group. These findings warrant effective treatment modalities to reduce the occurrence of oropharyngeal candidiasis.

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U2 - 10.1016/j.diabres.2017.01.001

DO - 10.1016/j.diabres.2017.01.001

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JO - Diabetes Research and Clinical Practice

JF - Diabetes Research and Clinical Practice

SN - 0168-8227

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