Ovarian development in wistar rat treated prenatally with single dose diisobutyl phthalate

B. Ray, A. S. D'Souza, V. Kumar, B. Pugazhandhi, M. R. D'Souza, D. Nayak, R. K. Sushma, P. Shetty, H. Singh, L. Krishna, K. M.R. Bhat, A. C.K. Rao, S. Chakraborti, N. Kumar, A. Saxena

Research output: Contribution to journalArticle

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Abstract

Phthalates are a class of industrial compounds with an array of toxicological properties used in day to day life. Diisobutyl phthalate on (DIBP) is used as an additive to keep the plastics soft or flexible (plasticizer) in nitrocellulose plastic, nail polish, explosives, lacquer manufacturing etc. Although DIBP exposure in humans is generally low, people in adhesive industries and pharmaceutical industries are exposed to higher levels. The aim of this study was to determine the effect of single dose of DIBP on developing ovary of Wistar rat. One hundred and eight adult pregnant Wistar rats were divided into control and experimental groups. Rats in experimental group were given DIBP on day 10, 12 and 14 of gestation at 0.375, 0.75 and 1.25 ml/kg body weight dose intraperitoneally in a single dose. Sections of ovaries collected on day 21 of gestation were stained with hematoxylin and eosin and examined and Masson's trichrome histologically. Sections belonging to the control group showed the presence of oocytes in clusters separated by thin fibrous septa. Degeneration oocytes, empty follicles surrounded by follicular cells without gonocytes in the center were observed in ovarian stroma. Blood vessels in the ovarian stroma were prominent and congested. Around a bunch of follicles total architectural disarray was observed although on special staining fibrosis was not evident. As pregnant women are constantly exposed, effect of DIBP on ovary of a developing fetus would denote the long term consequence in future generations.

Original languageEnglish
Pages (from-to)577-582
Number of pages6
JournalBratislava Medical Journal
Volume113
Issue number10
DOIs
Publication statusPublished - 2012

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Wistar Rats
Ovary
Plastics
Oocytes
Lacquer
Industrial Oils
Plasticizers
Pregnancy
Control Groups
Collodion
Social Responsibility
Drug Industry
Hematoxylin
Eosine Yellowish-(YS)
Nails
Adhesives
Toxicology
Blood Vessels
Pregnant Women
Industry

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Ray, B., D'Souza, A. S., Kumar, V., Pugazhandhi, B., D'Souza, M. R., Nayak, D., ... Saxena, A. (2012). Ovarian development in wistar rat treated prenatally with single dose diisobutyl phthalate. Bratislava Medical Journal, 113(10), 577-582. https://doi.org/10.4149/BLL_2012_129
Ray, B. ; D'Souza, A. S. ; Kumar, V. ; Pugazhandhi, B. ; D'Souza, M. R. ; Nayak, D. ; Sushma, R. K. ; Shetty, P. ; Singh, H. ; Krishna, L. ; Bhat, K. M.R. ; Rao, A. C.K. ; Chakraborti, S. ; Kumar, N. ; Saxena, A. / Ovarian development in wistar rat treated prenatally with single dose diisobutyl phthalate. In: Bratislava Medical Journal. 2012 ; Vol. 113, No. 10. pp. 577-582.
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Ray, B, D'Souza, AS, Kumar, V, Pugazhandhi, B, D'Souza, MR, Nayak, D, Sushma, RK, Shetty, P, Singh, H, Krishna, L, Bhat, KMR, Rao, ACK, Chakraborti, S, Kumar, N & Saxena, A 2012, 'Ovarian development in wistar rat treated prenatally with single dose diisobutyl phthalate', Bratislava Medical Journal, vol. 113, no. 10, pp. 577-582. https://doi.org/10.4149/BLL_2012_129

Ovarian development in wistar rat treated prenatally with single dose diisobutyl phthalate. / Ray, B.; D'Souza, A. S.; Kumar, V.; Pugazhandhi, B.; D'Souza, M. R.; Nayak, D.; Sushma, R. K.; Shetty, P.; Singh, H.; Krishna, L.; Bhat, K. M.R.; Rao, A. C.K.; Chakraborti, S.; Kumar, N.; Saxena, A.

In: Bratislava Medical Journal, Vol. 113, No. 10, 2012, p. 577-582.

Research output: Contribution to journalArticle

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AU - Ray, B.

AU - D'Souza, A. S.

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AU - Pugazhandhi, B.

AU - D'Souza, M. R.

AU - Nayak, D.

AU - Sushma, R. K.

AU - Shetty, P.

AU - Singh, H.

AU - Krishna, L.

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AU - Rao, A. C.K.

AU - Chakraborti, S.

AU - Kumar, N.

AU - Saxena, A.

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