Ovarian folliculogenesis

Detrimental effect of prenatal exposure to cyclophosphamide: A preliminary study

B. Ray, B. K. Potu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aim: To investigate whether cyclophosphamide interferes with ovarian folliculogenesis. Methods: In this experiment, pregnant rats (n=12) were randomly assigned into two groups, control group (n=6) and cyclophosphamide treatment group (n=6). In the cyclophosphamide treatment group cyclophosphamide was injected intraperitoneally from day 10 of gestation till 20th day, at 2 mg/kg of body weight. The pregnant rats were sacrificed on gestation day 20 and the fetus was collected. The collected fetuses were processed for sectioning and stained with haematoxyline and eosin for microscopic observation of the ovaries. Results: A meshwork-like appearance of mesenchyme with decreased number of somatic cells and absence of the majority of the germ cells in the ovarian follicles were found in treated fetus. Non-availability of primordial germ cells stopped the interaction between primordial germ cells and somatic supporting cells leading to non-proliferation and degeneration of somatic cells and fluid-filled vacant spaces in the meshwork -like arrangement of mesenchymal cells. Conclusion: We conclude that cyclophosphamide exposure prevents folliculogenesis by causing anovulation and results in infertility. The same detrimental effect might be seen in human fertility with environmental pollutants which are also metabolites of the drug.

Original languageEnglish
Pages (from-to)369-372
Number of pages4
JournalBratislava Medical Journal
Volume111
Issue number7
Publication statusPublished - 07-09-2010
Externally publishedYes

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Cyclophosphamide
Germ Cells
Fetus
Anovulation
Pregnancy
Environmental Pollutants
Ovarian Follicle
Mesoderm
Eosine Yellowish-(YS)
Cell Communication
Infertility
Fertility
Ovary
Cell Count
Body Weight
Control Groups
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "Aim: To investigate whether cyclophosphamide interferes with ovarian folliculogenesis. Methods: In this experiment, pregnant rats (n=12) were randomly assigned into two groups, control group (n=6) and cyclophosphamide treatment group (n=6). In the cyclophosphamide treatment group cyclophosphamide was injected intraperitoneally from day 10 of gestation till 20th day, at 2 mg/kg of body weight. The pregnant rats were sacrificed on gestation day 20 and the fetus was collected. The collected fetuses were processed for sectioning and stained with haematoxyline and eosin for microscopic observation of the ovaries. Results: A meshwork-like appearance of mesenchyme with decreased number of somatic cells and absence of the majority of the germ cells in the ovarian follicles were found in treated fetus. Non-availability of primordial germ cells stopped the interaction between primordial germ cells and somatic supporting cells leading to non-proliferation and degeneration of somatic cells and fluid-filled vacant spaces in the meshwork -like arrangement of mesenchymal cells. Conclusion: We conclude that cyclophosphamide exposure prevents folliculogenesis by causing anovulation and results in infertility. The same detrimental effect might be seen in human fertility with environmental pollutants which are also metabolites of the drug.",
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Ovarian folliculogenesis : Detrimental effect of prenatal exposure to cyclophosphamide: A preliminary study. / Ray, B.; Potu, B. K.

In: Bratislava Medical Journal, Vol. 111, No. 7, 07.09.2010, p. 369-372.

Research output: Contribution to journalArticle

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