Ovarian tissue vitrification is more efficient than slow freezing in protecting oocyte and granulosa cell DNA integrity

Freya Jothsna Mathias, Fiona D'souza, Shubhashree Uppangala, Sujith Raj Salian, Guruprasad Kalthur, Satish Kumar Adiga

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ovarian tissue cryopreservation is the primary treatment modality currently available to women at risk of losing their ovarian function due to cytotoxic therapy. However, the impact of these techniques on the oocyte DNA integrity is not elucidated. Here we have investigated the effect of vitrification and conventional slow freezing of eight week old Swiss albino mouse ovarian tissues on the oocyte and granulosa cell DNA integrity using the comet assay. The intracellular levels of reactive oxygen species in oocytes was measured by 2′,7′-dichlorodihydrofluorescein diacetate fluorescence. The cryopreservation of ovarian tissue by the slow freezing technique resulted in a significantly higher level of DNA fragmentation in oocytes in comparison to vitrification (p<0.05) whereas DNA fragmentation in granulosa cells was significantly higher than the control (p<0.01). Further, reactive oxygen species were significantly elevated in oocytes derived from slow freezing when compared to vitrification (p<0.05). Therefore, we conclude that the ovarian tissue slow freeze-thawing makes the oocyte and granulosa cells more vulnerable to DNA damage whereas vitrification appears to be a safer method than slow freezing for ovarian tissue cryopreservation.

Original languageEnglish
Pages (from-to)317-322
Number of pages6
JournalSystems Biology in Reproductive Medicine
Volume60
Issue number6
DOIs
Publication statusPublished - 01-12-2014

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Vitrification
Granulosa Cells
Freezing
Oocytes
DNA
Cryopreservation
DNA Fragmentation
Reactive Oxygen Species
Comet Assay
DNA Damage
Fluorescence
Therapeutics

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Urology
  • Medicine(all)

Cite this

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Ovarian tissue vitrification is more efficient than slow freezing in protecting oocyte and granulosa cell DNA integrity. / Mathias, Freya Jothsna; D'souza, Fiona; Uppangala, Shubhashree; Salian, Sujith Raj; Kalthur, Guruprasad; Adiga, Satish Kumar.

In: Systems Biology in Reproductive Medicine, Vol. 60, No. 6, 01.12.2014, p. 317-322.

Research output: Contribution to journalArticle

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AU - Kalthur, Guruprasad

AU - Adiga, Satish Kumar

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