p21 provides stage specific DNA damage control to preimplantation embryos

S. K. Adiga, M. Toyoshima, K. Shiraishi, T. Shimura, J. Takeda, M. Taga, H. Nagai, Pratap Kumar, O. Niwa

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The early stage embryogenesis of higher eukaryotes lacks some of the damage response pathways such as G1/S checkpoint, G2/M checkpoint and apoptosis. We examined here the damage response of preimplantation stage embryos after fertilization with 6 Gy irradiated sperm. Sperm-irradiated embryos developed normally for the first 2.5 days, but started to exhibit a developmental delay at day 3.5. p21 was activated in the delayed embryos, which carried numerous micronuclei owing to delayed chromosome instability. Apoptosis was observed predominantly in the inner cell mass of the day 4.0 embryos. Sperm-irradiated p21-/- embryos lacked the delay, but chromosome instability and apoptosis were more pronounced than the corresponding p21 wild-type embryos. We conclude from the result that damage responses come in a stage-specific manner during preimplantation stage development; p53-dependent S checkpoint at the zygote stage, p21-mediated cell cycle arrest at the morula/blastocyst stages and apoptosis after the blastocyst stage in the inner cell mass.

Original languageEnglish
Pages (from-to)6141-6149
Number of pages9
JournalOncogene
Volume26
Issue number42
DOIs
Publication statusPublished - 13-09-2007

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Blastocyst
DNA Damage
Embryonic Structures
Apoptosis
Spermatozoa
Chromosomal Instability
Morula
Zygote
Cell Cycle Checkpoints
Eukaryota
Fertilization
Embryonic Development

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Adiga, S. K., Toyoshima, M., Shiraishi, K., Shimura, T., Takeda, J., Taga, M., ... Niwa, O. (2007). p21 provides stage specific DNA damage control to preimplantation embryos. Oncogene, 26(42), 6141-6149. https://doi.org/10.1038/sj.onc.1210444
Adiga, S. K. ; Toyoshima, M. ; Shiraishi, K. ; Shimura, T. ; Takeda, J. ; Taga, M. ; Nagai, H. ; Kumar, Pratap ; Niwa, O. / p21 provides stage specific DNA damage control to preimplantation embryos. In: Oncogene. 2007 ; Vol. 26, No. 42. pp. 6141-6149.
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Adiga, SK, Toyoshima, M, Shiraishi, K, Shimura, T, Takeda, J, Taga, M, Nagai, H, Kumar, P & Niwa, O 2007, 'p21 provides stage specific DNA damage control to preimplantation embryos', Oncogene, vol. 26, no. 42, pp. 6141-6149. https://doi.org/10.1038/sj.onc.1210444

p21 provides stage specific DNA damage control to preimplantation embryos. / Adiga, S. K.; Toyoshima, M.; Shiraishi, K.; Shimura, T.; Takeda, J.; Taga, M.; Nagai, H.; Kumar, Pratap; Niwa, O.

In: Oncogene, Vol. 26, No. 42, 13.09.2007, p. 6141-6149.

Research output: Contribution to journalArticle

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Adiga SK, Toyoshima M, Shiraishi K, Shimura T, Takeda J, Taga M et al. p21 provides stage specific DNA damage control to preimplantation embryos. Oncogene. 2007 Sep 13;26(42):6141-6149. https://doi.org/10.1038/sj.onc.1210444