Paraffin baiting system for demonstration of growth and biofilm production in pseudomonas aeruginosa

Vishnu Prasad, Mamatha Ballal, P. G. Shivananda

Research output: Contribution to journalArticle

Abstract

Pseudomonas aeruginosa is one of the commonest pathogens among the pseudomonads. This organism can grow in minimal nutritional requirements. Because of the ability of pseudomonads to grow on paraffin is not commonly found among other human pathogens and the primary human pathogen being P. aeruginosa, we studied the adaptation of this organism to paraffin baiting system for growth and biofilm formation. Strains were tested for the capacity to use paraffin as the sole source of carbon using Czapek's minimal salt medium. Of the 53 clinical isolates of P. aeruginosa, 20 strains exhibited growth by 24 hrs and 42 strains by 48 hrs. The remaining strains did not show any growth in the paraffin baiting system. The oxidase test with the paraffin baiting system was also performed. This simple and inexpensive method can be used to isolate and demonstrate the biochemical and biofilm forming capacity of the organism.

Original languageEnglish
Pages (from-to)258-260
Number of pages3
JournalIndian Journal of Experimental Biology
Volume45
Issue number3
Publication statusPublished - 01-03-2007

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Biofilms
Paraffin
Pseudomonas aeruginosa
Growth
Nutritional Requirements
Oxidoreductases
Carbon
Salts

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

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Paraffin baiting system for demonstration of growth and biofilm production in pseudomonas aeruginosa. / Prasad, Vishnu; Ballal, Mamatha; Shivananda, P. G.

In: Indian Journal of Experimental Biology, Vol. 45, No. 3, 01.03.2007, p. 258-260.

Research output: Contribution to journalArticle

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