Pathological and biochemical evaluation of toxic effects of repeated administration of Jasada Bhasma (Zinc ash formulation) in wistar albino rats

Ravi Bhaskar, Megha A. Doshi, Anjana Shidaraddi, Shakunthala Ramdas Pai, Naveen Kumar, Ravi Shankar, Ravi Mundugaru

Research output: Contribution to journalArticle

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Abstract

Introduction: Jasada Bhasma is the well known potent zinc formulation commonly used in ‘Ayurveda’ medicine system. Its extensive usage in the health care sector is proved to be successful in the treatment of certain illness and is considered to be popular in its stability, lower dose, and its availability. Aim: To evaluate the toxicity profile of Jasada Bhasma with primary emphasis on haematological, biochemical and histopathological changes in Wistar albino rats. Materials and Methods: For toxicity study, Wistar albino rats of either sex, weighing from 150-225 gm of body weight were used, and they were divided into three groups, ten rats in each group. Group-I administered with carboxymethyl cellulose and considered as vehicle control. Group-II and III were administered with two levels of Jasada Bhasma, i.e., 10.8 mg/kg (Therapeutic dose) and 54 mg/kg (Five times of therapeutic dose) respectively for 28 consecutive days. On the 28th day, the animals were sacrificed. Blood sample and vital organs were collected for haematological, biochemical and histopathological examinations. Results: The repeated administration of Jasada Bhasma on Wistar albino rats did not produce remarkable toxicity changes at both the dose levels in most of the haematological parameters tested except there was a significant (p<0.01) increase in the mean cell haemoglobin concentration. Biochemically, the significant increase (p<0.01) in the serum creatinine but decrease in urea level has been shown in both levels of administration. The significant rise in potassium and total protein levels were also evident. Histopathologically, sections of liver and kidney showed pathological cytoarchitecture at both level dose exposures. However, sections of spleen showed mild to moderate increase in white pulp proportionate the five times of therapeutic dose level. Conclusion: The repeated administration of test drug Jasada Bhasma at therapeutic dose was relatively safer; however, at higher dose level it produces some functional changes in histopathological sections of kidney and liver.

Original languageEnglish
Pages (from-to)AC01-AC04
JournalJournal of Clinical and Diagnostic Research
Volume12
Issue number7
DOIs
Publication statusPublished - 01-07-2018

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Ashes
Poisons
Wistar Rats
Zinc
Rats
Toxicity
Liver
Kidney
Carboxymethylcellulose Sodium
Health Care Sector
Erythrocyte Indices
Weighing
Therapeutics
Systems Analysis
Health care
Medicine
Pulp
Urea
Creatinine
Potassium

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Bhaskar, Ravi ; Doshi, Megha A. ; Shidaraddi, Anjana ; Pai, Shakunthala Ramdas ; Kumar, Naveen ; Shankar, Ravi ; Mundugaru, Ravi. / Pathological and biochemical evaluation of toxic effects of repeated administration of Jasada Bhasma (Zinc ash formulation) in wistar albino rats. In: Journal of Clinical and Diagnostic Research. 2018 ; Vol. 12, No. 7. pp. AC01-AC04.
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Pathological and biochemical evaluation of toxic effects of repeated administration of Jasada Bhasma (Zinc ash formulation) in wistar albino rats. / Bhaskar, Ravi; Doshi, Megha A.; Shidaraddi, Anjana; Pai, Shakunthala Ramdas; Kumar, Naveen; Shankar, Ravi; Mundugaru, Ravi.

In: Journal of Clinical and Diagnostic Research, Vol. 12, No. 7, 01.07.2018, p. AC01-AC04.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Pathological and biochemical evaluation of toxic effects of repeated administration of Jasada Bhasma (Zinc ash formulation) in wistar albino rats

AU - Bhaskar, Ravi

AU - Doshi, Megha A.

AU - Shidaraddi, Anjana

AU - Pai, Shakunthala Ramdas

AU - Kumar, Naveen

AU - Shankar, Ravi

AU - Mundugaru, Ravi

PY - 2018/7/1

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N2 - Introduction: Jasada Bhasma is the well known potent zinc formulation commonly used in ‘Ayurveda’ medicine system. Its extensive usage in the health care sector is proved to be successful in the treatment of certain illness and is considered to be popular in its stability, lower dose, and its availability. Aim: To evaluate the toxicity profile of Jasada Bhasma with primary emphasis on haematological, biochemical and histopathological changes in Wistar albino rats. Materials and Methods: For toxicity study, Wistar albino rats of either sex, weighing from 150-225 gm of body weight were used, and they were divided into three groups, ten rats in each group. Group-I administered with carboxymethyl cellulose and considered as vehicle control. Group-II and III were administered with two levels of Jasada Bhasma, i.e., 10.8 mg/kg (Therapeutic dose) and 54 mg/kg (Five times of therapeutic dose) respectively for 28 consecutive days. On the 28th day, the animals were sacrificed. Blood sample and vital organs were collected for haematological, biochemical and histopathological examinations. Results: The repeated administration of Jasada Bhasma on Wistar albino rats did not produce remarkable toxicity changes at both the dose levels in most of the haematological parameters tested except there was a significant (p<0.01) increase in the mean cell haemoglobin concentration. Biochemically, the significant increase (p<0.01) in the serum creatinine but decrease in urea level has been shown in both levels of administration. The significant rise in potassium and total protein levels were also evident. Histopathologically, sections of liver and kidney showed pathological cytoarchitecture at both level dose exposures. However, sections of spleen showed mild to moderate increase in white pulp proportionate the five times of therapeutic dose level. Conclusion: The repeated administration of test drug Jasada Bhasma at therapeutic dose was relatively safer; however, at higher dose level it produces some functional changes in histopathological sections of kidney and liver.

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