PCR for M. tuberculosis in tissue samples

Kiran Chawla, Soham Gupta, Chiranjay Mukhopadhyay, P. Sugandhi Rao, Sudha S. Bhat

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: The presence of granuloma, visualized in histopathology for diagnosing tuberculosis in tissue samples, is not a specific finding. Moreover, histopathological examination of tissue sections needs one to two weeks for final reporting. A rapid and sensitive method is therefore needed for detection of Mycobacterium tuberculosis in these paucibacillary tissue samples. Methodology: A PCR-assay specific for IS6110 was evaluated for 104 different tissue samples in comparison to histopathology that was considered gold standard. Results: PCR showed 74.1% sensitivity and 96.1% specificity. False positive and false negative results were observed in three (2.88%) and seven (6.73%) samples, respectively. Positive agreement between histopathology and PCR was observed as 0.737, indicating substantial good agreement between two tests. Conclusions: PCR can be used for early diagnosis of tuberculosis in tissue samples that can help to initiate timely anti-tubercular treatment and prevent progression to irreversible changes.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalJournal of Infection in Developing Countries
Volume3
Issue number2
Publication statusPublished - 08-06-2009

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Tuberculosis
Polymerase Chain Reaction
Granuloma
Mycobacterium tuberculosis
Early Diagnosis
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Infectious Diseases
  • Virology

Cite this

Chawla, Kiran ; Gupta, Soham ; Mukhopadhyay, Chiranjay ; Rao, P. Sugandhi ; Bhat, Sudha S. / PCR for M. tuberculosis in tissue samples. In: Journal of Infection in Developing Countries. 2009 ; Vol. 3, No. 2. pp. 83-87.
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PCR for M. tuberculosis in tissue samples. / Chawla, Kiran; Gupta, Soham; Mukhopadhyay, Chiranjay; Rao, P. Sugandhi; Bhat, Sudha S.

In: Journal of Infection in Developing Countries, Vol. 3, No. 2, 08.06.2009, p. 83-87.

Research output: Contribution to journalArticle

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AU - Chawla, Kiran

AU - Gupta, Soham

AU - Mukhopadhyay, Chiranjay

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