PCR for M. tuberculosis in tissue samples

Kiran Chawla, Soham Gupta, Chiranjay Mukhopadhyay, P. Sugandhi Rao, Sudha S. Bhat

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: The presence of granuloma, visualized in histopathology for diagnosing tuberculosis in tissue samples, is not a specific finding. Moreover, histopathological examination of tissue sections needs one to two weeks for final reporting. A rapid and sensitive method is therefore needed for detection of Mycobacterium tuberculosis in these paucibacillary tissue samples. Methodology: A PCR-assay specific for IS6110 was evaluated for 104 different tissue samples in comparison to histopathology that was considered gold standard. Results: PCR showed 74.1% sensitivity and 96.1% specificity. False positive and false negative results were observed in three (2.88%) and seven (6.73%) samples, respectively. Positive agreement between histopathology and PCR was observed as 0.737, indicating substantial good agreement between two tests. Conclusions: PCR can be used for early diagnosis of tuberculosis in tissue samples that can help to initiate timely anti-tubercular treatment and prevent progression to irreversible changes.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalJournal of Infection in Developing Countries
Volume3
Issue number2
Publication statusPublished - 08-06-2009

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Infectious Diseases
  • Virology

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