Perform or perish: Laboratory optimization to avoid false negative blood cultures in pneumococcal bacteremia

Vandana Kalwaje Eshwara, Tushar Shaw, Yasha Mukim, Gauri Kumar, Asha Kamath, Chiranjay Mukhopadhyay

Research output: Contribution to journalArticle

Abstract

We present the laboratory operations influencing the culture yield of pneumococci and suggest an alternate solution to the problem. Blood cultures of 136 adults and 19 pediatric patients were analyzed in two phases. In phase I, the laboratory operated only during weekdays but in phase II the laboratory provided 24-h services on all days. In phase I, successful bacterial isolation leading to testing of antimicrobial susceptibility was possible only in 51% of cases, while a significant 49% had failed to grow in subcultures due to autolysis resulting from delayed processing time. These false negative blood cultures were further confirmed as Streptococcus pneumoniae by commercial antigen tests. In phase II, we did not observe any false-negative results due to prompt subculturing protocols (p < 0.001). We also found that taking blood cultures on two occasions increases the yield by 46% among adults.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalJournal of Laboratory Medicine
Volume43
Issue number4
DOIs
Publication statusPublished - 01-08-2019

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Bacteremia
Blood
Streptococcus pneumoniae
Optimization
Autolysis
Pediatrics
Alternate
Susceptibility
Isolation
Antigens
Testing
Processing
False
Culture
Blood Culture

All Science Journal Classification (ASJC) codes

  • Discrete Mathematics and Combinatorics
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

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abstract = "We present the laboratory operations influencing the culture yield of pneumococci and suggest an alternate solution to the problem. Blood cultures of 136 adults and 19 pediatric patients were analyzed in two phases. In phase I, the laboratory operated only during weekdays but in phase II the laboratory provided 24-h services on all days. In phase I, successful bacterial isolation leading to testing of antimicrobial susceptibility was possible only in 51{\%} of cases, while a significant 49{\%} had failed to grow in subcultures due to autolysis resulting from delayed processing time. These false negative blood cultures were further confirmed as Streptococcus pneumoniae by commercial antigen tests. In phase II, we did not observe any false-negative results due to prompt subculturing protocols (p < 0.001). We also found that taking blood cultures on two occasions increases the yield by 46{\%} among adults.",
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Perform or perish : Laboratory optimization to avoid false negative blood cultures in pneumococcal bacteremia. / Eshwara, Vandana Kalwaje; Shaw, Tushar; Mukim, Yasha; Kumar, Gauri; Kamath, Asha; Mukhopadhyay, Chiranjay.

In: Journal of Laboratory Medicine, Vol. 43, No. 4, 01.08.2019, p. 217-220.

Research output: Contribution to journalArticle

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