Petroleum ether extract of Cissus quadrangularis (Linn) stimulates the growth of fetal bone during intra uterine developmental period: A morphometric analysis

Bhagath Kumar Potu, Muddanna S. Rao, G. Kutty N, Kumar M.R. Bhat, Mallikarjuna Rao Chamallamudi, Soubhagya Ranjan Nayak

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

OBJECTIVE: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats. METHODS: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software. RESULTS: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001-0.0001) than that of the control group. CONCLUSION: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

Original languageEnglish
Pages (from-to)815-820
Number of pages6
JournalClinics
Volume63
Issue number6
DOIs
Publication statusPublished - 2008

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Cissus
Bone Development
Fetal Development
Cartilage
Control Groups
Bone and Bones
Pregnancy
Alcian Blue
Software
Body Weight
Mothers
naphtha
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Potu, Bhagath Kumar ; Rao, Muddanna S. ; Kutty N, G. ; Bhat, Kumar M.R. ; Chamallamudi, Mallikarjuna Rao ; Nayak, Soubhagya Ranjan. / Petroleum ether extract of Cissus quadrangularis (Linn) stimulates the growth of fetal bone during intra uterine developmental period : A morphometric analysis. In: Clinics. 2008 ; Vol. 63, No. 6. pp. 815-820.
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abstract = "OBJECTIVE: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats. METHODS: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software. RESULTS: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001-0.0001) than that of the control group. CONCLUSION: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.",
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Petroleum ether extract of Cissus quadrangularis (Linn) stimulates the growth of fetal bone during intra uterine developmental period : A morphometric analysis. / Potu, Bhagath Kumar; Rao, Muddanna S.; Kutty N, G.; Bhat, Kumar M.R.; Chamallamudi, Mallikarjuna Rao; Nayak, Soubhagya Ranjan.

In: Clinics, Vol. 63, No. 6, 2008, p. 815-820.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Petroleum ether extract of Cissus quadrangularis (Linn) stimulates the growth of fetal bone during intra uterine developmental period

T2 - A morphometric analysis

AU - Potu, Bhagath Kumar

AU - Rao, Muddanna S.

AU - Kutty N, G.

AU - Bhat, Kumar M.R.

AU - Chamallamudi, Mallikarjuna Rao

AU - Nayak, Soubhagya Ranjan

PY - 2008

Y1 - 2008

N2 - OBJECTIVE: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats. METHODS: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software. RESULTS: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001-0.0001) than that of the control group. CONCLUSION: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

AB - OBJECTIVE: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats. METHODS: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software. RESULTS: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001-0.0001) than that of the control group. CONCLUSION: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

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