Petroleum ether extract of Cissus quadrangularis (Linn) stimulates the growth of fetal bone during intra uterine developmental period: A morphometric analysis

Bhagath Kumar Potu, Muddanna S. Rao, G. Kutty N, Kumar M.R. Bhat, Mallikarjuna Rao Chamallamudi, Soubhagya Ranjan Nayak

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OBJECTIVE: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats. METHODS: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software. RESULTS: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001-0.0001) than that of the control group. CONCLUSION: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

Original languageEnglish
Pages (from-to)815-820
Number of pages6
Issue number6
Publication statusPublished - 2008


All Science Journal Classification (ASJC) codes

  • Medicine(all)

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