Pharmacological approach to increasing the retention of radiation-induced γ-H2AX foci using phosphatase inhibitors

Significance in radiation biodosimetry

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    1 Citation (Scopus)

    Abstract

    In a scenario of accidental mass radiation exposure transportation and analysis of samples may take some time, resulting in loss of biomarker information over this period. The present study aims to use phosphatase inhibitors for longer retention of focal signals to adopt γ-H2AX as a biodosimetric biomarker for the management of early triage. Peripheral blood lymphocytes isolated from healthy individuals were irradiated in vitro with x-rays and γ-H2AX foci were analysed using fluorescent microscopy and flow cytometric methods. Further, the effect of protein phosphatase 2A inhibitors such as calyculin A, fostriecin and okadiac acid on the retention of foci was studied. Fluorescent microscopy was found to be a more sensitive method than flow cytometry. Calyculin A showed significant retention of focal signals at 6 h with 1.5-fold increased retention compared to radiation alone; this may prove beneficial in early triage management because of a better dose approximation.

    Original languageEnglish
    Pages (from-to)318-328
    Number of pages11
    JournalJournal of Radiological Protection
    Volume38
    Issue number1
    DOIs
    Publication statusPublished - 01-03-2018

    Fingerprint

    Triage
    Phosphoric Monoester Hydrolases
    phosphatase
    Microscopy
    inhibitor
    Biomarkers
    Pharmacology
    Radiation
    Protein Phosphatase 2
    biomarker
    microscopy
    Flow Cytometry
    X-Rays
    Lymphocytes
    Acids
    radiation exposure
    flow cytometry
    blood
    fold
    protein

    All Science Journal Classification (ASJC) codes

    • Waste Management and Disposal
    • Public Health, Environmental and Occupational Health

    Cite this

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    title = "Pharmacological approach to increasing the retention of radiation-induced γ-H2AX foci using phosphatase inhibitors: Significance in radiation biodosimetry",
    abstract = "In a scenario of accidental mass radiation exposure transportation and analysis of samples may take some time, resulting in loss of biomarker information over this period. The present study aims to use phosphatase inhibitors for longer retention of focal signals to adopt γ-H2AX as a biodosimetric biomarker for the management of early triage. Peripheral blood lymphocytes isolated from healthy individuals were irradiated in vitro with x-rays and γ-H2AX foci were analysed using fluorescent microscopy and flow cytometric methods. Further, the effect of protein phosphatase 2A inhibitors such as calyculin A, fostriecin and okadiac acid on the retention of foci was studied. Fluorescent microscopy was found to be a more sensitive method than flow cytometry. Calyculin A showed significant retention of focal signals at 6 h with 1.5-fold increased retention compared to radiation alone; this may prove beneficial in early triage management because of a better dose approximation.",
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