11 Citations (Scopus)

Abstract

The present work reports the photo-biomodulatory effect of red (632.8 nm) and near infrared (785 and 830 nm) lasers on burn injury in Swiss albino mice. Animals were induced with a 15-mm full thickness burn injury and irradiated with various fluences (1, 2, 3, 4, and 6 J/cm2) of each laser wavelength under study having a constant fluence rate (8.49 mW/cm2). The size of the injury following treatment was monitored by capturing the wound images at regular time intervals until complete healing. Morphometric assessment indicated that the group treated with 3-J/cm2 fluence of 830 nm had a profound effect on healing as compared to untreated controls and various fluences of other wavelengths under study. Histopathological assessment of wound repair on treatment with an optimum fluence (3 J/cm2) of 830 nm performed on days 2, 6, 12, and 18 post-wounding resulted in enhanced wound repair with migration of fibroblasts, deposition of collagen, and neovascularization as compared to untreated controls. The findings of the present study have clearly demonstrated that a single exposure of 3-J/cm2 fluence at 830-nm enhanced burn wound healing progression in mice, which is equivalent to 5 % povidone iodine treatment (reference standard), applied on a daily basis till complete healing.

Original languageEnglish
Pages (from-to)1741-1750
Number of pages10
JournalLasers in Medical Science
Volume31
Issue number9
DOIs
Publication statusPublished - 01-12-2016

Fingerprint

Wounds and Injuries
Lasers
Povidone-Iodine
Wound Healing
Low-Level Light Therapy
Collagen
Therapeutics
Fibroblasts

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

Rathnakar, Bharath ; Rao, Bola Sadashiva Satish ; Prabhu, Vijendra ; Chandra, Subhash ; Rai, Sharada ; Rao, Anuradha Calicut Kini ; Sharma, Mrinalini ; Gupta, Pradeep Kumar ; Mahato, Krishna Kishore. / Photo-biomodulatory response of low-power laser irradiation on burn tissue repair in mice. In: Lasers in Medical Science. 2016 ; Vol. 31, No. 9. pp. 1741-1750.
@article{17a18359dae94eb9b1ad70b54bfd81b7,
title = "Photo-biomodulatory response of low-power laser irradiation on burn tissue repair in mice",
abstract = "The present work reports the photo-biomodulatory effect of red (632.8 nm) and near infrared (785 and 830 nm) lasers on burn injury in Swiss albino mice. Animals were induced with a 15-mm full thickness burn injury and irradiated with various fluences (1, 2, 3, 4, and 6 J/cm2) of each laser wavelength under study having a constant fluence rate (8.49 mW/cm2). The size of the injury following treatment was monitored by capturing the wound images at regular time intervals until complete healing. Morphometric assessment indicated that the group treated with 3-J/cm2 fluence of 830 nm had a profound effect on healing as compared to untreated controls and various fluences of other wavelengths under study. Histopathological assessment of wound repair on treatment with an optimum fluence (3 J/cm2) of 830 nm performed on days 2, 6, 12, and 18 post-wounding resulted in enhanced wound repair with migration of fibroblasts, deposition of collagen, and neovascularization as compared to untreated controls. The findings of the present study have clearly demonstrated that a single exposure of 3-J/cm2 fluence at 830-nm enhanced burn wound healing progression in mice, which is equivalent to 5 {\%} povidone iodine treatment (reference standard), applied on a daily basis till complete healing.",
author = "Bharath Rathnakar and Rao, {Bola Sadashiva Satish} and Vijendra Prabhu and Subhash Chandra and Sharada Rai and Rao, {Anuradha Calicut Kini} and Mrinalini Sharma and Gupta, {Pradeep Kumar} and Mahato, {Krishna Kishore}",
year = "2016",
month = "12",
day = "1",
doi = "10.1007/s10103-016-2044-2",
language = "English",
volume = "31",
pages = "1741--1750",
journal = "Lasers in Medical Science",
issn = "0268-8921",
publisher = "Springer London",
number = "9",

}

Photo-biomodulatory response of low-power laser irradiation on burn tissue repair in mice. / Rathnakar, Bharath; Rao, Bola Sadashiva Satish; Prabhu, Vijendra; Chandra, Subhash; Rai, Sharada; Rao, Anuradha Calicut Kini; Sharma, Mrinalini; Gupta, Pradeep Kumar; Mahato, Krishna Kishore.

In: Lasers in Medical Science, Vol. 31, No. 9, 01.12.2016, p. 1741-1750.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Photo-biomodulatory response of low-power laser irradiation on burn tissue repair in mice

AU - Rathnakar, Bharath

AU - Rao, Bola Sadashiva Satish

AU - Prabhu, Vijendra

AU - Chandra, Subhash

AU - Rai, Sharada

AU - Rao, Anuradha Calicut Kini

AU - Sharma, Mrinalini

AU - Gupta, Pradeep Kumar

AU - Mahato, Krishna Kishore

PY - 2016/12/1

Y1 - 2016/12/1

N2 - The present work reports the photo-biomodulatory effect of red (632.8 nm) and near infrared (785 and 830 nm) lasers on burn injury in Swiss albino mice. Animals were induced with a 15-mm full thickness burn injury and irradiated with various fluences (1, 2, 3, 4, and 6 J/cm2) of each laser wavelength under study having a constant fluence rate (8.49 mW/cm2). The size of the injury following treatment was monitored by capturing the wound images at regular time intervals until complete healing. Morphometric assessment indicated that the group treated with 3-J/cm2 fluence of 830 nm had a profound effect on healing as compared to untreated controls and various fluences of other wavelengths under study. Histopathological assessment of wound repair on treatment with an optimum fluence (3 J/cm2) of 830 nm performed on days 2, 6, 12, and 18 post-wounding resulted in enhanced wound repair with migration of fibroblasts, deposition of collagen, and neovascularization as compared to untreated controls. The findings of the present study have clearly demonstrated that a single exposure of 3-J/cm2 fluence at 830-nm enhanced burn wound healing progression in mice, which is equivalent to 5 % povidone iodine treatment (reference standard), applied on a daily basis till complete healing.

AB - The present work reports the photo-biomodulatory effect of red (632.8 nm) and near infrared (785 and 830 nm) lasers on burn injury in Swiss albino mice. Animals were induced with a 15-mm full thickness burn injury and irradiated with various fluences (1, 2, 3, 4, and 6 J/cm2) of each laser wavelength under study having a constant fluence rate (8.49 mW/cm2). The size of the injury following treatment was monitored by capturing the wound images at regular time intervals until complete healing. Morphometric assessment indicated that the group treated with 3-J/cm2 fluence of 830 nm had a profound effect on healing as compared to untreated controls and various fluences of other wavelengths under study. Histopathological assessment of wound repair on treatment with an optimum fluence (3 J/cm2) of 830 nm performed on days 2, 6, 12, and 18 post-wounding resulted in enhanced wound repair with migration of fibroblasts, deposition of collagen, and neovascularization as compared to untreated controls. The findings of the present study have clearly demonstrated that a single exposure of 3-J/cm2 fluence at 830-nm enhanced burn wound healing progression in mice, which is equivalent to 5 % povidone iodine treatment (reference standard), applied on a daily basis till complete healing.

UR - http://www.scopus.com/inward/record.url?scp=84982890717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84982890717&partnerID=8YFLogxK

U2 - 10.1007/s10103-016-2044-2

DO - 10.1007/s10103-016-2044-2

M3 - Article

VL - 31

SP - 1741

EP - 1750

JO - Lasers in Medical Science

JF - Lasers in Medical Science

SN - 0268-8921

IS - 9

ER -