Abstract

Low power laser irradiation (LPLI) is becoming an increasingly popular and fast growing therapeutic modality in dermatology to treat various ailments without any reported side effects. The present study was intended to examine the effect of He-Ne laser induced changes on cellular parameters in wounded monolayer human skin fibroblast cells grown in-vitro. To achieve the stated objective, uniform scratch (central scratch) was created on confluent monolayered human skin fibroblast cells, and were exposed to single dose of He-Ne laser (15 mm spot, 6.68 mWcm-2) at 5 and 7 Jcm-2 in the presence of 10 % fetal bovine serum (FBS) along with appropriate sham irradiated controls. Upon laser irradiation cell apoptosis, cell proliferation and mitochondrial membrane potential were documented at various time intervals. Immediate exposure of laser at 5 Jcm-2 significantly attenuated the cell apoptosis, induced drastic increase in mitochondrial membrane potential in wounded monolayer human skin fibroblast cells compared to sham irradiated controls. Further, single exposure of red light to wounded monolayer human skin fibroblast cells at 5 Jcm-2 significantly increased the cell proliferation compared to sham irradiated group. The findings of the present study demonstrated beneficial effects of He-Ne laser in wounded monolayer human skin fibroblast cells in a dose dependent manner. In conclusion, the low power laser irradiation augment various cellular processes in wounded monolayer human skin fibroblast cells resulting in enhanced tissue regeneration.

Original languageEnglish
Title of host publicationInternational Conference on Fibre Optics and Photonics, Photonics 2016
PublisherOSA - The Optical Society
ISBN (Print)9781943580224
DOIs
Publication statusPublished - 21-07-2014
EventInternational Conference on Fibre Optics and Photonics, Photonics 2016 - Kanpur, India
Duration: 04-12-201608-12-2016

Conference

ConferenceInternational Conference on Fibre Optics and Photonics, Photonics 2016
CountryIndia
CityKanpur
Period04-12-1608-12-16

Fingerprint

Fibroblasts
Skin
Cells
Monolayers
Lasers
Laser beam effects
Cell proliferation
Cell death
Dermatology
Apoptosis
Membranes
Tissue regeneration

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

Cite this

Prabhu, V., Rao, B. S. S., & Mahato, K. K. (2014). Photobiomodulatory effects of he- ne laser on wounded human skin fibroblasts. In International Conference on Fibre Optics and Photonics, Photonics 2016 OSA - The Optical Society. https://doi.org/10.1364/PHOTONICS.2016.Th3A.3
Prabhu, Vijendra ; Rao, Bola Sadashiva Satish ; Mahato, Krishna Kishore. / Photobiomodulatory effects of he- ne laser on wounded human skin fibroblasts. International Conference on Fibre Optics and Photonics, Photonics 2016. OSA - The Optical Society, 2014.
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abstract = "Low power laser irradiation (LPLI) is becoming an increasingly popular and fast growing therapeutic modality in dermatology to treat various ailments without any reported side effects. The present study was intended to examine the effect of He-Ne laser induced changes on cellular parameters in wounded monolayer human skin fibroblast cells grown in-vitro. To achieve the stated objective, uniform scratch (central scratch) was created on confluent monolayered human skin fibroblast cells, and were exposed to single dose of He-Ne laser (15 mm spot, 6.68 mWcm-2) at 5 and 7 Jcm-2 in the presence of 10 {\%} fetal bovine serum (FBS) along with appropriate sham irradiated controls. Upon laser irradiation cell apoptosis, cell proliferation and mitochondrial membrane potential were documented at various time intervals. Immediate exposure of laser at 5 Jcm-2 significantly attenuated the cell apoptosis, induced drastic increase in mitochondrial membrane potential in wounded monolayer human skin fibroblast cells compared to sham irradiated controls. Further, single exposure of red light to wounded monolayer human skin fibroblast cells at 5 Jcm-2 significantly increased the cell proliferation compared to sham irradiated group. The findings of the present study demonstrated beneficial effects of He-Ne laser in wounded monolayer human skin fibroblast cells in a dose dependent manner. In conclusion, the low power laser irradiation augment various cellular processes in wounded monolayer human skin fibroblast cells resulting in enhanced tissue regeneration.",
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Prabhu, V, Rao, BSS & Mahato, KK 2014, Photobiomodulatory effects of he- ne laser on wounded human skin fibroblasts. in International Conference on Fibre Optics and Photonics, Photonics 2016. OSA - The Optical Society, International Conference on Fibre Optics and Photonics, Photonics 2016, Kanpur, India, 04-12-16. https://doi.org/10.1364/PHOTONICS.2016.Th3A.3

Photobiomodulatory effects of he- ne laser on wounded human skin fibroblasts. / Prabhu, Vijendra; Rao, Bola Sadashiva Satish; Mahato, Krishna Kishore.

International Conference on Fibre Optics and Photonics, Photonics 2016. OSA - The Optical Society, 2014.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Rao, Bola Sadashiva Satish

AU - Mahato, Krishna Kishore

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AB - Low power laser irradiation (LPLI) is becoming an increasingly popular and fast growing therapeutic modality in dermatology to treat various ailments without any reported side effects. The present study was intended to examine the effect of He-Ne laser induced changes on cellular parameters in wounded monolayer human skin fibroblast cells grown in-vitro. To achieve the stated objective, uniform scratch (central scratch) was created on confluent monolayered human skin fibroblast cells, and were exposed to single dose of He-Ne laser (15 mm spot, 6.68 mWcm-2) at 5 and 7 Jcm-2 in the presence of 10 % fetal bovine serum (FBS) along with appropriate sham irradiated controls. Upon laser irradiation cell apoptosis, cell proliferation and mitochondrial membrane potential were documented at various time intervals. Immediate exposure of laser at 5 Jcm-2 significantly attenuated the cell apoptosis, induced drastic increase in mitochondrial membrane potential in wounded monolayer human skin fibroblast cells compared to sham irradiated controls. Further, single exposure of red light to wounded monolayer human skin fibroblast cells at 5 Jcm-2 significantly increased the cell proliferation compared to sham irradiated group. The findings of the present study demonstrated beneficial effects of He-Ne laser in wounded monolayer human skin fibroblast cells in a dose dependent manner. In conclusion, the low power laser irradiation augment various cellular processes in wounded monolayer human skin fibroblast cells resulting in enhanced tissue regeneration.

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Prabhu V, Rao BSS, Mahato KK. Photobiomodulatory effects of he- ne laser on wounded human skin fibroblasts. In International Conference on Fibre Optics and Photonics, Photonics 2016. OSA - The Optical Society. 2014 https://doi.org/10.1364/PHOTONICS.2016.Th3A.3