Polarization resolved second harmonic microscopy

Nirmal Mazumder, Gitanjal Deka, Wei Wen Wu, Ankur Gogoi, Guan Yu Zhuo, Fu Jen Kao

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

Second harmonic (SH) microscopy has proven to be a powerful imaging modality over the past years due to its intrinsic advantages as a multiphoton process with endogenous contrast specificity, which allows pinhole-less optical sectioning, non-invasive observation, deep tissue penetration, and the possibility of easier signal detection at visible wavelengths. Depending on the relative orientation between the polarization of the incoming light and the second-order susceptibility of non-centrosymmetric structures, SH microscopy provides the unique capacity to probe the absolute molecular structure of a broad variety of biological tissues without the necessity for additional labeling. In addition, SH microscopy, when working with polarimetry, provides clear and in-depth insights on the details of molecular orientation and structural symmetry. In this review, the working principles of the polarization resolving techniques and the corresponding implements of SH microscopy are elucidated, with focus on Stokes vector based polarimetry. An overview of the advancements on SH anisotropy measurements are also presented. Specifically, the recent progresses on the following three topics in polarization resolved SH microscopy will be elucidated, which include Stokes vector resolving for imaging molecular structure and orientation, 3-D structural chirality by SH circular dichroism, and correlation with fluorescence lifetime imaging (FLIM) for in vivo wound healing diagnosis. The potentials and challenges for future researches in exploring complex biological tissues are also discussed.

Original languageEnglish
Pages (from-to)105-118
Number of pages14
JournalMethods
Volume128
DOIs
Publication statusPublished - 01-09-2017

Fingerprint

Microscopy
Microscopic examination
Polarization
Polarimeters
Molecular orientation
Tissue
Molecular Structure
Molecular structure
Multiphoton processes
Imaging techniques
Chirality
Optical Imaging
Anisotropy
Signal detection
Circular Dichroism
Wound Healing
Labeling
Fluorescence
Observation
Light

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mazumder, N., Deka, G., Wu, W. W., Gogoi, A., Zhuo, G. Y., & Kao, F. J. (2017). Polarization resolved second harmonic microscopy. Methods, 128, 105-118. https://doi.org/10.1016/j.ymeth.2017.06.012
Mazumder, Nirmal ; Deka, Gitanjal ; Wu, Wei Wen ; Gogoi, Ankur ; Zhuo, Guan Yu ; Kao, Fu Jen. / Polarization resolved second harmonic microscopy. In: Methods. 2017 ; Vol. 128. pp. 105-118.
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Mazumder, N, Deka, G, Wu, WW, Gogoi, A, Zhuo, GY & Kao, FJ 2017, 'Polarization resolved second harmonic microscopy', Methods, vol. 128, pp. 105-118. https://doi.org/10.1016/j.ymeth.2017.06.012

Polarization resolved second harmonic microscopy. / Mazumder, Nirmal; Deka, Gitanjal; Wu, Wei Wen; Gogoi, Ankur; Zhuo, Guan Yu; Kao, Fu Jen.

In: Methods, Vol. 128, 01.09.2017, p. 105-118.

Research output: Contribution to journalReview article

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