Potentiation of hydroxyurea cytotoxicity in human chronic myeloid leukemia cells by iron-chelating agent

K. Satyamoorthy, Manik P. Chitnis, Vathsala S. Basrur, Suresh H. Advani

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    The effect of hydroxyurea (HU) was studied in 15 cases of human chronic myeloid leukemia cells (CML) in combination with iron-chelating agent, 2,2-bipyridine (bipyridine). The extent of (3H)thymidine incorporation in CML cells in vitro was taken as an index for the measurement of cytotoxicity. In the present study, we observed a potentiation in HU cytotoxicity by hydrophobic iron-chelator bipyridine (p < 0.001) resulting in complete DNA biosynthesis inhibition. Both HU and bipyridine were used at a relatively non-toxic concentrations of 10-4M and 10 μg/ml, respectively. This inhibition was found to be partially reversible and a partial protective action by iron is also demonstrated. The various other metal chelators failed to sensitise CML cells to HU cytotoxicity. Implications with respect to the efficacy in cancer treatment is discussed.

    Original languageEnglish
    Pages (from-to)1327-1330
    Number of pages4
    JournalLeukemia Research
    Volume10
    Issue number11
    DOIs
    Publication statusPublished - 1986

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    Iron Chelating Agents
    Hydroxyurea
    Myeloid Cells
    Leukemia, Myelogenous, Chronic, BCR-ABL Positive
    Chelating Agents
    Iron
    Thymidine
    Metals
    DNA
    Neoplasms

    All Science Journal Classification (ASJC) codes

    • Hematology
    • Oncology
    • Cancer Research

    Cite this

    Satyamoorthy, K. ; Chitnis, Manik P. ; Basrur, Vathsala S. ; Advani, Suresh H. / Potentiation of hydroxyurea cytotoxicity in human chronic myeloid leukemia cells by iron-chelating agent. In: Leukemia Research. 1986 ; Vol. 10, No. 11. pp. 1327-1330.
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    abstract = "The effect of hydroxyurea (HU) was studied in 15 cases of human chronic myeloid leukemia cells (CML) in combination with iron-chelating agent, 2,2-bipyridine (bipyridine). The extent of (3H)thymidine incorporation in CML cells in vitro was taken as an index for the measurement of cytotoxicity. In the present study, we observed a potentiation in HU cytotoxicity by hydrophobic iron-chelator bipyridine (p < 0.001) resulting in complete DNA biosynthesis inhibition. Both HU and bipyridine were used at a relatively non-toxic concentrations of 10-4M and 10 μg/ml, respectively. This inhibition was found to be partially reversible and a partial protective action by iron is also demonstrated. The various other metal chelators failed to sensitise CML cells to HU cytotoxicity. Implications with respect to the efficacy in cancer treatment is discussed.",
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    Potentiation of hydroxyurea cytotoxicity in human chronic myeloid leukemia cells by iron-chelating agent. / Satyamoorthy, K.; Chitnis, Manik P.; Basrur, Vathsala S.; Advani, Suresh H.

    In: Leukemia Research, Vol. 10, No. 11, 1986, p. 1327-1330.

    Research output: Contribution to journalArticle

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    AU - Advani, Suresh H.

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    AB - The effect of hydroxyurea (HU) was studied in 15 cases of human chronic myeloid leukemia cells (CML) in combination with iron-chelating agent, 2,2-bipyridine (bipyridine). The extent of (3H)thymidine incorporation in CML cells in vitro was taken as an index for the measurement of cytotoxicity. In the present study, we observed a potentiation in HU cytotoxicity by hydrophobic iron-chelator bipyridine (p < 0.001) resulting in complete DNA biosynthesis inhibition. Both HU and bipyridine were used at a relatively non-toxic concentrations of 10-4M and 10 μg/ml, respectively. This inhibition was found to be partially reversible and a partial protective action by iron is also demonstrated. The various other metal chelators failed to sensitise CML cells to HU cytotoxicity. Implications with respect to the efficacy in cancer treatment is discussed.

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