Preclinical pharmacokinetics and biodistribution studies of asenapine maleate using novel and sensitive RP-HPLC method

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aim: Asenapine maleate (ASPM) is a newer antipsychotic drug available as a sublingual tablet in the market. Experimental: To investigate the pharmacokinetic and tissue distribution study of ASPM following oral administration in rats, reversed-phase HPLC method was developed and validated. Results: ASPM was extracted from plasma and tissue matrix by liquid-liquid extraction technique and analyzed using mobile phase consisted of phosphate buffer pH 3.0 and acetonitrile (65:35% v/v). The method showed good linearity (10-500 ng/ml) with recovery 83-102%. In pharmacokinetics study, half-life was 32.74 ± 7.51 h due to slow elimination of drug. The biodistribution study indicated preferential distribution of ASPM to highly perfused organs. Conclusion: The current method can be successfully applied for estimating the drug in various biological matrices.

Original languageEnglish
Pages (from-to)1037-1047
Number of pages11
JournalBioanalysis
Volume9
Issue number14
DOIs
Publication statusPublished - 01-07-2017

Fingerprint

Pharmacokinetics
High Pressure Liquid Chromatography
Tissue
Liquid-Liquid Extraction
Liquids
Tissue Distribution
Pharmaceutical Preparations
Antipsychotic Agents
Tablets
Oral Administration
Half-Life
Rats
Buffers
Phosphates
Plasmas
Recovery
Asenapine

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

@article{69a09672814f4ae78f0a3f7696b7e534,
title = "Preclinical pharmacokinetics and biodistribution studies of asenapine maleate using novel and sensitive RP-HPLC method",
abstract = "Aim: Asenapine maleate (ASPM) is a newer antipsychotic drug available as a sublingual tablet in the market. Experimental: To investigate the pharmacokinetic and tissue distribution study of ASPM following oral administration in rats, reversed-phase HPLC method was developed and validated. Results: ASPM was extracted from plasma and tissue matrix by liquid-liquid extraction technique and analyzed using mobile phase consisted of phosphate buffer pH 3.0 and acetonitrile (65:35{\%} v/v). The method showed good linearity (10-500 ng/ml) with recovery 83-102{\%}. In pharmacokinetics study, half-life was 32.74 ± 7.51 h due to slow elimination of drug. The biodistribution study indicated preferential distribution of ASPM to highly perfused organs. Conclusion: The current method can be successfully applied for estimating the drug in various biological matrices.",
author = "Managuli, {Renuka S.} and Karthik Gourishetti and Shenoy, {Rekha R.} and Koteshwara, {Kunnatur B.} and Reddy, {Meka Sreenivasa} and Srinivas Mutalik",
year = "2017",
month = "7",
day = "1",
doi = "10.4155/bio-2017-0069",
language = "English",
volume = "9",
pages = "1037--1047",
journal = "Bioanalysis",
issn = "1757-6180",
publisher = "Future Science",
number = "14",

}

Preclinical pharmacokinetics and biodistribution studies of asenapine maleate using novel and sensitive RP-HPLC method. / Managuli, Renuka S.; Gourishetti, Karthik; Shenoy, Rekha R.; Koteshwara, Kunnatur B.; Reddy, Meka Sreenivasa; Mutalik, Srinivas.

In: Bioanalysis, Vol. 9, No. 14, 01.07.2017, p. 1037-1047.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preclinical pharmacokinetics and biodistribution studies of asenapine maleate using novel and sensitive RP-HPLC method

AU - Managuli, Renuka S.

AU - Gourishetti, Karthik

AU - Shenoy, Rekha R.

AU - Koteshwara, Kunnatur B.

AU - Reddy, Meka Sreenivasa

AU - Mutalik, Srinivas

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Aim: Asenapine maleate (ASPM) is a newer antipsychotic drug available as a sublingual tablet in the market. Experimental: To investigate the pharmacokinetic and tissue distribution study of ASPM following oral administration in rats, reversed-phase HPLC method was developed and validated. Results: ASPM was extracted from plasma and tissue matrix by liquid-liquid extraction technique and analyzed using mobile phase consisted of phosphate buffer pH 3.0 and acetonitrile (65:35% v/v). The method showed good linearity (10-500 ng/ml) with recovery 83-102%. In pharmacokinetics study, half-life was 32.74 ± 7.51 h due to slow elimination of drug. The biodistribution study indicated preferential distribution of ASPM to highly perfused organs. Conclusion: The current method can be successfully applied for estimating the drug in various biological matrices.

AB - Aim: Asenapine maleate (ASPM) is a newer antipsychotic drug available as a sublingual tablet in the market. Experimental: To investigate the pharmacokinetic and tissue distribution study of ASPM following oral administration in rats, reversed-phase HPLC method was developed and validated. Results: ASPM was extracted from plasma and tissue matrix by liquid-liquid extraction technique and analyzed using mobile phase consisted of phosphate buffer pH 3.0 and acetonitrile (65:35% v/v). The method showed good linearity (10-500 ng/ml) with recovery 83-102%. In pharmacokinetics study, half-life was 32.74 ± 7.51 h due to slow elimination of drug. The biodistribution study indicated preferential distribution of ASPM to highly perfused organs. Conclusion: The current method can be successfully applied for estimating the drug in various biological matrices.

UR - http://www.scopus.com/inward/record.url?scp=85026526055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026526055&partnerID=8YFLogxK

U2 - 10.4155/bio-2017-0069

DO - 10.4155/bio-2017-0069

M3 - Article

VL - 9

SP - 1037

EP - 1047

JO - Bioanalysis

JF - Bioanalysis

SN - 1757-6180

IS - 14

ER -