Preclinical pharmacokinetics and biodistribution studies of asenapine maleate using novel and sensitive RP-HPLC method

Renuka S. Managuli, Karthik Gourishetti, Rekha R. Shenoy, Kunnatur B. Koteshwara, Meka Sreenivasa Reddy, Srinivas Mutalik

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aim: Asenapine maleate (ASPM) is a newer antipsychotic drug available as a sublingual tablet in the market. Experimental: To investigate the pharmacokinetic and tissue distribution study of ASPM following oral administration in rats, reversed-phase HPLC method was developed and validated. Results: ASPM was extracted from plasma and tissue matrix by liquid-liquid extraction technique and analyzed using mobile phase consisted of phosphate buffer pH 3.0 and acetonitrile (65:35% v/v). The method showed good linearity (10-500 ng/ml) with recovery 83-102%. In pharmacokinetics study, half-life was 32.74 ± 7.51 h due to slow elimination of drug. The biodistribution study indicated preferential distribution of ASPM to highly perfused organs. Conclusion: The current method can be successfully applied for estimating the drug in various biological matrices.

Original languageEnglish
Pages (from-to)1037-1047
Number of pages11
JournalBioanalysis
Volume9
Issue number14
DOIs
Publication statusPublished - 01-07-2017

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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