Prediction of continuous reactors performance based on batch reactor deactivation kinetics data of immobilized lipase

V. Ramachandra Murty, Jayadev Bhat, P. K.A. Muniswaran

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4 Citations (Scopus)


Experiments on deactivation kinetics of immobilized lipase enzyme from Candida cylindracea were performed in stirred batch reactor using rice bran oil as the substrate and temperature as the deactivation parameter. The data were fitted in first order deactivation model. The effect of temperature on deactivation rate was represented by Arrhenius equation. Theoretical equations were developed based on pseudo-steady state approximation and Michaelis-Menten rate expression to predict the time course of conversion due to enzyme deactivation and apparent half-life of the immobilized enzyme activity in PFR and CSTR under constant feed rate policy for no diffusion limitation and diffusion limitation of first order. Stability of enzyme in these continuous reactors was predicted and factors affecting the stability were analyzed.

Original languageEnglish
Pages (from-to)225-230
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Issue number4
Publication statusPublished - 01-01-2002


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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