Preliminary evaluation of in vitro cytotoxicity and in vivo antitumor activity of xanthium strumarium in transplantable tumors in mice

Jesil Mathew Aranjani, Atulya Manuel, Chamallamudi Mallikarjuna Rao, Nayanabhirama Udupa, Josyula Venkata Rao, Ann Mary Joy, Prajay Gandhi, Ethiraj Kannat Radhakrishnan

Research output: Contribution to journalArticle

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Abstract

In the present study, active fractions of the methanolic extract of Xanthium strumarium (XS) showing potent cytotoxicity were determined using microculture tetrazolium (MTT) and sulforhodamine B (SRB) assays in selected cancer cell lines. The active fractions viz., chloroform soluble fraction of root (CEXSR), hexane soluble fraction of leaf (HEXSL), hexane soluble fraction of fruits (HEXSF) and chloroform soluble fraction of fruits (CEXSF) of XS were tested in transplantable animal tumor models for their antitumor potential. Dalton's ascitic lymphoma (DLA) cells were used to induce solid and liquid (ascites) tumor in mice. The tumor bearing animals were treated with active fractions at two dose levels (100 and 200 mg/kg). The antitumor activities of the active fractions in tumor bearing animals were monitored with parameters such as body weight and increase in life-span as well as biochemical and hematological modalities (in the case of liquid tumor). Tumor incidence and tumor volume were the parameters monitored in the case of the solid tumor model. The results were analyzed by one-way ANOVA followed by Tukey's post hoc test. The extracts were found to increase the life-span of tumor bearing animals and restore the altered hematological and biochemical parameters significantly.

Original languageEnglish
Pages (from-to)145-162
Number of pages18
JournalAmerican Journal of Chinese Medicine
Volume41
Issue number1
DOIs
Publication statusPublished - 2013

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Xanthium
Neoplasms
Hexanes
Chloroform
lissamine rhodamine B
Fruit
In Vitro Techniques
Tumor Burden
Ascites
Lymphoma
Analysis of Variance
Animal Models
Body Weight

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

Cite this

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title = "Preliminary evaluation of in vitro cytotoxicity and in vivo antitumor activity of xanthium strumarium in transplantable tumors in mice",
abstract = "In the present study, active fractions of the methanolic extract of Xanthium strumarium (XS) showing potent cytotoxicity were determined using microculture tetrazolium (MTT) and sulforhodamine B (SRB) assays in selected cancer cell lines. The active fractions viz., chloroform soluble fraction of root (CEXSR), hexane soluble fraction of leaf (HEXSL), hexane soluble fraction of fruits (HEXSF) and chloroform soluble fraction of fruits (CEXSF) of XS were tested in transplantable animal tumor models for their antitumor potential. Dalton's ascitic lymphoma (DLA) cells were used to induce solid and liquid (ascites) tumor in mice. The tumor bearing animals were treated with active fractions at two dose levels (100 and 200 mg/kg). The antitumor activities of the active fractions in tumor bearing animals were monitored with parameters such as body weight and increase in life-span as well as biochemical and hematological modalities (in the case of liquid tumor). Tumor incidence and tumor volume were the parameters monitored in the case of the solid tumor model. The results were analyzed by one-way ANOVA followed by Tukey's post hoc test. The extracts were found to increase the life-span of tumor bearing animals and restore the altered hematological and biochemical parameters significantly.",
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Preliminary evaluation of in vitro cytotoxicity and in vivo antitumor activity of xanthium strumarium in transplantable tumors in mice. / Aranjani, Jesil Mathew; Manuel, Atulya; Mallikarjuna Rao, Chamallamudi; Udupa, Nayanabhirama; Rao, Josyula Venkata; Joy, Ann Mary; Gandhi, Prajay; Radhakrishnan, Ethiraj Kannat.

In: American Journal of Chinese Medicine, Vol. 41, No. 1, 2013, p. 145-162.

Research output: Contribution to journalArticle

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AU - Aranjani, Jesil Mathew

AU - Manuel, Atulya

AU - Mallikarjuna Rao, Chamallamudi

AU - Udupa, Nayanabhirama

AU - Rao, Josyula Venkata

AU - Joy, Ann Mary

AU - Gandhi, Prajay

AU - Radhakrishnan, Ethiraj Kannat

PY - 2013

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