Preliminary study on the antisnake venom activity of alcoholic root extract of Clerodendrum viscosum (Vent.) in Naja naja Venom

R. Lobo, I.S.R. Punitha, K. Rajendran, A. Shirwaikar

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The antisnake venom activity of Clerodendrum viscosum Vent. (Fam. Verbenaceae), a plant traditionally used in India for the treatment of snake bite was evaluated by in vitro and in vivo methods. While in vitro studies were performed using human blood, in vivo studies were carried out using mice administered three different i.p doses of the extract, 5 min before the administration of Naja naja snake venom. The results of the in vitro studies showed that the extract probably interacts with but does not stabilize membrane protein. In the in vivo studies, the extract showed significant antisnake venom activity, which may be attributed to its possible interference with the acetylcholine receptor sites. Hence the present investigation justifies the traditional use of Clerodendrum viscosum (C. viscosum) as antisnake venom.
Original languageEnglish
Pages (from-to)153-156
Number of pages4
JournalNatural Product Sciences
Volume12
Issue number3
Publication statusPublished - 2006

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Clerodendrum
Cobra Venoms
Elapidae
Vents
Venoms
Verbenaceae
Snake Bites
Snake Venoms
Cholinergic Receptors
India
Membrane Proteins
Blood
In Vitro Techniques

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title = "Preliminary study on the antisnake venom activity of alcoholic root extract of Clerodendrum viscosum (Vent.) in Naja naja Venom",
abstract = "The antisnake venom activity of Clerodendrum viscosum Vent. (Fam. Verbenaceae), a plant traditionally used in India for the treatment of snake bite was evaluated by in vitro and in vivo methods. While in vitro studies were performed using human blood, in vivo studies were carried out using mice administered three different i.p doses of the extract, 5 min before the administration of Naja naja snake venom. The results of the in vitro studies showed that the extract probably interacts with but does not stabilize membrane protein. In the in vivo studies, the extract showed significant antisnake venom activity, which may be attributed to its possible interference with the acetylcholine receptor sites. Hence the present investigation justifies the traditional use of Clerodendrum viscosum (C. viscosum) as antisnake venom.",
author = "R. Lobo and I.S.R. Punitha and K. Rajendran and A. Shirwaikar",
note = "Cited By :6 Export Date: 10 November 2017 CODEN: NPSCF Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 576104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: snake venom, 55230-69-8 References: Abe, H., Katada, K., Orita, M., Nishikibe, M., Effects of calcium antagonists on the etythrocyte membrane (1991) J. Pharm. Pharmacol., 43 (1), pp. 22-26; Alam, M.I., Auddy, B., Gomes, A., Isolation, Purification and partial characterization of Viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (1994) Toxicon., 32 (12), pp. 1551-1557; Nandy, A., (2000) The Essentials of Forensic Medicine and Toxicology, Principles of Forensic Medicine. 3rd Edition 2000, 2, pp. 507-515. , New Central Book Agency. (P) ltd. Calcutta-Ed; Balu, S., Alagesaboopathy. Antisnake venom activities of some species of Andrographis wall (1995) Ancient Sciences of Life, 14 (3), pp. 187-190; Chatterjee, S.C., Management of snake bite cases (1965) J. Ind. Med. Assoc., 45, p. 654; Ghosh, M.N., Toxicity studies (1984) Fundamentals of Experimental Pharmacology, pp. 153-158. , Scientific Book Agency, Calcutta; Gurumukh, S., Essentials of Forensic medicine (1999) API Textbook of Medicine, Ed. 6, pp. 1317-1320; Haque, N., Chowdhury, S.A., Nutan, M.T., Evaluation of antitumor activity of some medicinal plants of Bangladesh by potato disk bioassay (2000) Fitoterapia, 71 (5), pp. 547-552; Harborne, J.B., (1984) Phytochemical Methods, 2nd Edition, pp. 123-175. , Chapmann and Hill, London; Haruna, A.K., Chowdhury, M.K., In vivo antisnake venom of a furanoid diterpene from Aristolochia albida (1995) Ind. J. Pharm. Sci., 57 (5), pp. 222-224; Kokate, C.K., Purohit, A.P., Gokhale, S.B., (1965) Textbook of Practical Pharmacognosy, Nirali Prakashan, 9th Edition, pp. 90-93; Maeno, H., Mitsuhashi, S., Okonogi, T., Hoshi, S., Homma, M., Studies on Habu snake venom (V). Myolysis caused by phospholipase A in Habu snake venom (1962) Jap. J. Exp. Med., 32, pp. 55-64; Motta, R., Passive immunotherapy of leukaemia and other cancer (1971) Adv. Cancer Res., 14, pp. 161-179; Murugesh, N., Vembar, S., Damodaran, C., Studies on erythrocyte membrane IV: In vitro haemolytic activity of oleander extract (1981) Toxicol. Lett., 8, pp. 33-38; Nadkarni, A.K., (1981) Indian Materia Medica, 13th Edition, pp. 284-285. , Dhootapapeshwar Prakashan Ltd., Bombay; Houghton, P.J., Osibogum, I.M., Flowering plants used against snake bite (1993) J. Ethnopharmacol., 39 (1), pp. 1-29; Roelofsen, B., Zwaal, R.F., Comfurius, P., Woodward, C.B., Von Deenen, L.L., Action of pure phospholipase A 2 and Phospholipase C on human erythrocytes and ghosts (1971) Biochem. Biophys. Acta, 241 (3), pp. 925-929; Rosenberg, P., (1979) Snake Venom, pp. 403-447. , Springer. Verlag, New York; Turner, M.A., (1965) Screening Methods in Pharmacology, p. 26. , Academic Press, New York; Warrel, D.A., Manson's Tropical diseases (1987) Balliere Tindall, 9, p. 855; Warrier, P.K., Nambiar, V.P.K., Raman Kutty, C., (1996) Indian Medicinal Plants, 1, p. 160. , Orient Longman, Hyderabad, India; Yoganarasimhan, S.N., (2000) Medicinal Plants of India-Tamilnadu, 2, p. 48. , Bangalore",
year = "2006",
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Preliminary study on the antisnake venom activity of alcoholic root extract of Clerodendrum viscosum (Vent.) in Naja naja Venom. / Lobo, R.; Punitha, I.S.R.; Rajendran, K.; Shirwaikar, A.

In: Natural Product Sciences, Vol. 12, No. 3, 2006, p. 153-156.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preliminary study on the antisnake venom activity of alcoholic root extract of Clerodendrum viscosum (Vent.) in Naja naja Venom

AU - Lobo, R.

AU - Punitha, I.S.R.

AU - Rajendran, K.

AU - Shirwaikar, A.

N1 - Cited By :6 Export Date: 10 November 2017 CODEN: NPSCF Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 576104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: snake venom, 55230-69-8 References: Abe, H., Katada, K., Orita, M., Nishikibe, M., Effects of calcium antagonists on the etythrocyte membrane (1991) J. Pharm. Pharmacol., 43 (1), pp. 22-26; Alam, M.I., Auddy, B., Gomes, A., Isolation, Purification and partial characterization of Viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (1994) Toxicon., 32 (12), pp. 1551-1557; Nandy, A., (2000) The Essentials of Forensic Medicine and Toxicology, Principles of Forensic Medicine. 3rd Edition 2000, 2, pp. 507-515. , New Central Book Agency. (P) ltd. Calcutta-Ed; Balu, S., Alagesaboopathy. Antisnake venom activities of some species of Andrographis wall (1995) Ancient Sciences of Life, 14 (3), pp. 187-190; Chatterjee, S.C., Management of snake bite cases (1965) J. Ind. Med. Assoc., 45, p. 654; Ghosh, M.N., Toxicity studies (1984) Fundamentals of Experimental Pharmacology, pp. 153-158. , Scientific Book Agency, Calcutta; Gurumukh, S., Essentials of Forensic medicine (1999) API Textbook of Medicine, Ed. 6, pp. 1317-1320; Haque, N., Chowdhury, S.A., Nutan, M.T., Evaluation of antitumor activity of some medicinal plants of Bangladesh by potato disk bioassay (2000) Fitoterapia, 71 (5), pp. 547-552; Harborne, J.B., (1984) Phytochemical Methods, 2nd Edition, pp. 123-175. , Chapmann and Hill, London; Haruna, A.K., Chowdhury, M.K., In vivo antisnake venom of a furanoid diterpene from Aristolochia albida (1995) Ind. J. Pharm. Sci., 57 (5), pp. 222-224; Kokate, C.K., Purohit, A.P., Gokhale, S.B., (1965) Textbook of Practical Pharmacognosy, Nirali Prakashan, 9th Edition, pp. 90-93; Maeno, H., Mitsuhashi, S., Okonogi, T., Hoshi, S., Homma, M., Studies on Habu snake venom (V). Myolysis caused by phospholipase A in Habu snake venom (1962) Jap. J. Exp. Med., 32, pp. 55-64; Motta, R., Passive immunotherapy of leukaemia and other cancer (1971) Adv. Cancer Res., 14, pp. 161-179; Murugesh, N., Vembar, S., Damodaran, C., Studies on erythrocyte membrane IV: In vitro haemolytic activity of oleander extract (1981) Toxicol. Lett., 8, pp. 33-38; Nadkarni, A.K., (1981) Indian Materia Medica, 13th Edition, pp. 284-285. , Dhootapapeshwar Prakashan Ltd., Bombay; Houghton, P.J., Osibogum, I.M., Flowering plants used against snake bite (1993) J. Ethnopharmacol., 39 (1), pp. 1-29; Roelofsen, B., Zwaal, R.F., Comfurius, P., Woodward, C.B., Von Deenen, L.L., Action of pure phospholipase A 2 and Phospholipase C on human erythrocytes and ghosts (1971) Biochem. Biophys. Acta, 241 (3), pp. 925-929; Rosenberg, P., (1979) Snake Venom, pp. 403-447. , Springer. Verlag, New York; Turner, M.A., (1965) Screening Methods in Pharmacology, p. 26. , Academic Press, New York; Warrel, D.A., Manson's Tropical diseases (1987) Balliere Tindall, 9, p. 855; Warrier, P.K., Nambiar, V.P.K., Raman Kutty, C., (1996) Indian Medicinal Plants, 1, p. 160. , Orient Longman, Hyderabad, India; Yoganarasimhan, S.N., (2000) Medicinal Plants of India-Tamilnadu, 2, p. 48. , Bangalore

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AB - The antisnake venom activity of Clerodendrum viscosum Vent. (Fam. Verbenaceae), a plant traditionally used in India for the treatment of snake bite was evaluated by in vitro and in vivo methods. While in vitro studies were performed using human blood, in vivo studies were carried out using mice administered three different i.p doses of the extract, 5 min before the administration of Naja naja snake venom. The results of the in vitro studies showed that the extract probably interacts with but does not stabilize membrane protein. In the in vivo studies, the extract showed significant antisnake venom activity, which may be attributed to its possible interference with the acetylcholine receptor sites. Hence the present investigation justifies the traditional use of Clerodendrum viscosum (C. viscosum) as antisnake venom.

M3 - Article

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SP - 153

EP - 156

JO - Natural Product Sciences

JF - Natural Product Sciences

SN - 1226-3907

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ER -