Preparation and characterization of novel PBAE/PLGA polymer blend microparticles for DNA vaccine delivery

Meenashi Vanathi Balashanmugam, Sivagurunathan Nagarethinam, Hitesh Jagani, Venkata Rao Josyula, Abdulmohsen Alrohaimi, Nayanabhirama Udupa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Context. Poly(beta-amino ester) (PBAE) with its pH sensitiveness and Poly(lactic-co-glycolic acid) (PLGA) with huge DNA cargo capacity in combination prove to be highly efficient as DNA delivery system. Objective. To study the effectiveness of novel synthesized PBAE polymer with PLGA blend at different ratios in DNA vaccine delivery. Methods. In the present study, multifunctional polymer blend microparticles using a combination of PLGA and novel PBAE polymers A1 (bis(3-(propionyloxy)propyl)3,3′-(propane-1,3-diyl-bis(methylazanediyl))dipropanoate) and A2 (bis(4-(propionyloxy)butyl)3,3′-(ethane-1,2-diyl-bis(isopropylazanediyl))dipropanoate) at different ratios (85: 15, 75: 25, and 50: 50) were prepared by double emulsion solvent removal method. The microparticles were characterized for cytotoxicity, transfection efficiency, and DNA encapsulation efficiency. Result. It was evident from results that among the microparticles prepared with PLGA/PBAE blend the PLGA: PBAE at 85: 15 ratio was found to be more effective combination than the microparticles prepared with PLGA alone in terms of transfection efficiency and better DNA integrity. Microparticles made of PLGA and PBAE A1 at 85: 15 ratio, respectively, were found to be less toxic when compared with microparticles prepared with A2 polymer. Conclusion. The results encourage the use of the synthesized PBAE polymer in combination with PLGA as an effective gene delivery system.

Original languageEnglish
Article number385135
JournalScientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

DNA Vaccines
vaccine
Polymer blends
ester
Polymers
polymer
DNA
acid
varespladib methyl
Transfection
Gene Transfer Techniques
Propane
Ethane
encapsulation
poly(beta-amino ester)
polylactic acid-polyglycolic acid copolymer
Poisons
propane
ethane
cargo

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Balashanmugam, Meenashi Vanathi ; Nagarethinam, Sivagurunathan ; Jagani, Hitesh ; Josyula, Venkata Rao ; Alrohaimi, Abdulmohsen ; Udupa, Nayanabhirama. / Preparation and characterization of novel PBAE/PLGA polymer blend microparticles for DNA vaccine delivery. In: Scientific World Journal. 2014 ; Vol. 2014.
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Preparation and characterization of novel PBAE/PLGA polymer blend microparticles for DNA vaccine delivery. / Balashanmugam, Meenashi Vanathi; Nagarethinam, Sivagurunathan; Jagani, Hitesh; Josyula, Venkata Rao; Alrohaimi, Abdulmohsen; Udupa, Nayanabhirama.

In: Scientific World Journal, Vol. 2014, 385135, 2014.

Research output: Contribution to journalArticle

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AU - Balashanmugam, Meenashi Vanathi

AU - Nagarethinam, Sivagurunathan

AU - Jagani, Hitesh

AU - Josyula, Venkata Rao

AU - Alrohaimi, Abdulmohsen

AU - Udupa, Nayanabhirama

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N2 - Context. Poly(beta-amino ester) (PBAE) with its pH sensitiveness and Poly(lactic-co-glycolic acid) (PLGA) with huge DNA cargo capacity in combination prove to be highly efficient as DNA delivery system. Objective. To study the effectiveness of novel synthesized PBAE polymer with PLGA blend at different ratios in DNA vaccine delivery. Methods. In the present study, multifunctional polymer blend microparticles using a combination of PLGA and novel PBAE polymers A1 (bis(3-(propionyloxy)propyl)3,3′-(propane-1,3-diyl-bis(methylazanediyl))dipropanoate) and A2 (bis(4-(propionyloxy)butyl)3,3′-(ethane-1,2-diyl-bis(isopropylazanediyl))dipropanoate) at different ratios (85: 15, 75: 25, and 50: 50) were prepared by double emulsion solvent removal method. The microparticles were characterized for cytotoxicity, transfection efficiency, and DNA encapsulation efficiency. Result. It was evident from results that among the microparticles prepared with PLGA/PBAE blend the PLGA: PBAE at 85: 15 ratio was found to be more effective combination than the microparticles prepared with PLGA alone in terms of transfection efficiency and better DNA integrity. Microparticles made of PLGA and PBAE A1 at 85: 15 ratio, respectively, were found to be less toxic when compared with microparticles prepared with A2 polymer. Conclusion. The results encourage the use of the synthesized PBAE polymer in combination with PLGA as an effective gene delivery system.

AB - Context. Poly(beta-amino ester) (PBAE) with its pH sensitiveness and Poly(lactic-co-glycolic acid) (PLGA) with huge DNA cargo capacity in combination prove to be highly efficient as DNA delivery system. Objective. To study the effectiveness of novel synthesized PBAE polymer with PLGA blend at different ratios in DNA vaccine delivery. Methods. In the present study, multifunctional polymer blend microparticles using a combination of PLGA and novel PBAE polymers A1 (bis(3-(propionyloxy)propyl)3,3′-(propane-1,3-diyl-bis(methylazanediyl))dipropanoate) and A2 (bis(4-(propionyloxy)butyl)3,3′-(ethane-1,2-diyl-bis(isopropylazanediyl))dipropanoate) at different ratios (85: 15, 75: 25, and 50: 50) were prepared by double emulsion solvent removal method. The microparticles were characterized for cytotoxicity, transfection efficiency, and DNA encapsulation efficiency. Result. It was evident from results that among the microparticles prepared with PLGA/PBAE blend the PLGA: PBAE at 85: 15 ratio was found to be more effective combination than the microparticles prepared with PLGA alone in terms of transfection efficiency and better DNA integrity. Microparticles made of PLGA and PBAE A1 at 85: 15 ratio, respectively, were found to be less toxic when compared with microparticles prepared with A2 polymer. Conclusion. The results encourage the use of the synthesized PBAE polymer in combination with PLGA as an effective gene delivery system.

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