Prevalence of methicillin resistant staphylococcus aureus carriage amongst health care workers of critical care units in kasturba medical college hospital, mangalore, india

M. Radhakrishna, M. D'Souza, S. Kotigadde, K. V. Saralaya, M. S. Kotian

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Outbreaks and prevalence of Methicillin resistant Staphylococcus aureus (MRSA) Nosocomial Infection (NI) among various populations have been well reported in literature, particularly those from developed countries. There is a paucity of information on carriage of MRSA in developing nations, including the carriage by critical healthcare givers who are potential transmitters. Aim and Objectives: Present study was aimed at establishing the carriage rate of MRSA among healthcare workers in the critical care units of Kasturba Medical College Hospital, Mangalore, and at formulating an MRSA control policy, based on the outcomes. Material and Methods: We screened 200 healthcare workers in the critical care units of the Kasturba Medical College Hospital, Mangalore, India, for MRSA and vancomycin susceptibility of the isolates. Swabs taken from both anterior nares were transported, inoculated onto mannitol salt agar (MSA) and incubated aerobically at 37°C for 18-24 hours. Gram positive cocci in clusters, with positive catalase, coagulase and DNAse tests, were identified as S.aureus. Further categorization of S.aureus into MRSA was done by using cefoxitin disc diffusion method. Sensitivity to vancomycin was tested by vancomycin disc diffusion and vancomycin agar screen plating. Results: The number of strains of S. aureus which was isolated from our 200 participants was 35, with a rate of 17.5% of the 35 isolates of S. aureus, 5 (14.3%) were MRSA. None of the S. aureus strains were vancomycin resistant. Conclusion: MRSA carriage among healthcare workers who were involved in the management of critically ill patients at Kasturba Medical College hospital, Mangalore, India was 2.5%, which is comfortably low. The existing infection control policy in our hospital seems to be effective and the same should be maintained.

Original languageEnglish
Pages (from-to)2697-2699
Number of pages3
JournalJournal of Clinical and Diagnostic Research
Volume7
Issue number12
DOIs
Publication statusPublished - 15-12-2013

Fingerprint

Methicillin
Critical Care
Methicillin-Resistant Staphylococcus aureus
Health care
India
Delivery of Health Care
Vancomycin
Staphylococcus aureus
Agar
Cefoxitin
Gram-Positive Cocci
Coagulase
Mannitol
Infection Control
Cross Infection
Plating
Developed Countries
Critical Illness
Catalase
Developing Countries

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Clinical Biochemistry

Cite this

@article{75f41536e20140c5b73a172fed693e40,
title = "Prevalence of methicillin resistant staphylococcus aureus carriage amongst health care workers of critical care units in kasturba medical college hospital, mangalore, india",
abstract = "Background: Outbreaks and prevalence of Methicillin resistant Staphylococcus aureus (MRSA) Nosocomial Infection (NI) among various populations have been well reported in literature, particularly those from developed countries. There is a paucity of information on carriage of MRSA in developing nations, including the carriage by critical healthcare givers who are potential transmitters. Aim and Objectives: Present study was aimed at establishing the carriage rate of MRSA among healthcare workers in the critical care units of Kasturba Medical College Hospital, Mangalore, and at formulating an MRSA control policy, based on the outcomes. Material and Methods: We screened 200 healthcare workers in the critical care units of the Kasturba Medical College Hospital, Mangalore, India, for MRSA and vancomycin susceptibility of the isolates. Swabs taken from both anterior nares were transported, inoculated onto mannitol salt agar (MSA) and incubated aerobically at 37°C for 18-24 hours. Gram positive cocci in clusters, with positive catalase, coagulase and DNAse tests, were identified as S.aureus. Further categorization of S.aureus into MRSA was done by using cefoxitin disc diffusion method. Sensitivity to vancomycin was tested by vancomycin disc diffusion and vancomycin agar screen plating. Results: The number of strains of S. aureus which was isolated from our 200 participants was 35, with a rate of 17.5{\%} of the 35 isolates of S. aureus, 5 (14.3{\%}) were MRSA. None of the S. aureus strains were vancomycin resistant. Conclusion: MRSA carriage among healthcare workers who were involved in the management of critically ill patients at Kasturba Medical College hospital, Mangalore, India was 2.5{\%}, which is comfortably low. The existing infection control policy in our hospital seems to be effective and the same should be maintained.",
author = "M. Radhakrishna and M. D'Souza and S. Kotigadde and Saralaya, {K. V.} and Kotian, {M. S.}",
year = "2013",
month = "12",
day = "15",
doi = "10.7860/JCDR/2013/5160.3735",
language = "English",
volume = "7",
pages = "2697--2699",
journal = "Journal of Clinical and Diagnostic Research",
issn = "2249-782X",
publisher = "Journal of Clinical and Diagnostic Research",
number = "12",

}

TY - JOUR

T1 - Prevalence of methicillin resistant staphylococcus aureus carriage amongst health care workers of critical care units in kasturba medical college hospital, mangalore, india

AU - Radhakrishna, M.

AU - D'Souza, M.

AU - Kotigadde, S.

AU - Saralaya, K. V.

AU - Kotian, M. S.

PY - 2013/12/15

Y1 - 2013/12/15

N2 - Background: Outbreaks and prevalence of Methicillin resistant Staphylococcus aureus (MRSA) Nosocomial Infection (NI) among various populations have been well reported in literature, particularly those from developed countries. There is a paucity of information on carriage of MRSA in developing nations, including the carriage by critical healthcare givers who are potential transmitters. Aim and Objectives: Present study was aimed at establishing the carriage rate of MRSA among healthcare workers in the critical care units of Kasturba Medical College Hospital, Mangalore, and at formulating an MRSA control policy, based on the outcomes. Material and Methods: We screened 200 healthcare workers in the critical care units of the Kasturba Medical College Hospital, Mangalore, India, for MRSA and vancomycin susceptibility of the isolates. Swabs taken from both anterior nares were transported, inoculated onto mannitol salt agar (MSA) and incubated aerobically at 37°C for 18-24 hours. Gram positive cocci in clusters, with positive catalase, coagulase and DNAse tests, were identified as S.aureus. Further categorization of S.aureus into MRSA was done by using cefoxitin disc diffusion method. Sensitivity to vancomycin was tested by vancomycin disc diffusion and vancomycin agar screen plating. Results: The number of strains of S. aureus which was isolated from our 200 participants was 35, with a rate of 17.5% of the 35 isolates of S. aureus, 5 (14.3%) were MRSA. None of the S. aureus strains were vancomycin resistant. Conclusion: MRSA carriage among healthcare workers who were involved in the management of critically ill patients at Kasturba Medical College hospital, Mangalore, India was 2.5%, which is comfortably low. The existing infection control policy in our hospital seems to be effective and the same should be maintained.

AB - Background: Outbreaks and prevalence of Methicillin resistant Staphylococcus aureus (MRSA) Nosocomial Infection (NI) among various populations have been well reported in literature, particularly those from developed countries. There is a paucity of information on carriage of MRSA in developing nations, including the carriage by critical healthcare givers who are potential transmitters. Aim and Objectives: Present study was aimed at establishing the carriage rate of MRSA among healthcare workers in the critical care units of Kasturba Medical College Hospital, Mangalore, and at formulating an MRSA control policy, based on the outcomes. Material and Methods: We screened 200 healthcare workers in the critical care units of the Kasturba Medical College Hospital, Mangalore, India, for MRSA and vancomycin susceptibility of the isolates. Swabs taken from both anterior nares were transported, inoculated onto mannitol salt agar (MSA) and incubated aerobically at 37°C for 18-24 hours. Gram positive cocci in clusters, with positive catalase, coagulase and DNAse tests, were identified as S.aureus. Further categorization of S.aureus into MRSA was done by using cefoxitin disc diffusion method. Sensitivity to vancomycin was tested by vancomycin disc diffusion and vancomycin agar screen plating. Results: The number of strains of S. aureus which was isolated from our 200 participants was 35, with a rate of 17.5% of the 35 isolates of S. aureus, 5 (14.3%) were MRSA. None of the S. aureus strains were vancomycin resistant. Conclusion: MRSA carriage among healthcare workers who were involved in the management of critically ill patients at Kasturba Medical College hospital, Mangalore, India was 2.5%, which is comfortably low. The existing infection control policy in our hospital seems to be effective and the same should be maintained.

UR - http://www.scopus.com/inward/record.url?scp=84890453378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890453378&partnerID=8YFLogxK

U2 - 10.7860/JCDR/2013/5160.3735

DO - 10.7860/JCDR/2013/5160.3735

M3 - Article

VL - 7

SP - 2697

EP - 2699

JO - Journal of Clinical and Diagnostic Research

JF - Journal of Clinical and Diagnostic Research

SN - 2249-782X

IS - 12

ER -